Nrf2 12721

PBA was isolated from I. pubescens and identified by Dr. Peng Wu from Guangzhou University of Chinese Medicine. Antibodies against Keap1 (10503-2-AP) was purchased from Proteintech Group, Inc. (Chicago, IL, USA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, E12-052), HO-1 (E1A5393), NQO1 (E2A6437), cullin-3 (Cul3, E2A7225V), Flag (E1T508-2) and β-actin (E12-051) antibodies were purchased from Enogene Company (Nanjing, Jiangsu, China). Ubiquitin (Ub, sc-8017), caspase-1 (sc-56036), caspase-1 p20 (sc-398715) and interleukin-1 beta (IL-1β, sc-52012) antibodies were bought from Santa Cruz Biotechnology (Santa Cruz CA, USA). Nrf2 (12721) and lamin B1 (13435) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Nrf2 antibody (ab137550) for immunofluorescence staining was purchased from Abcam (Cambridge, UK). NLPR3 (NBP-2-12446) antibody was purchased from Novus Biologicals, LLC (Centennial, CO, USA). MG-132 (HY-13259) and tBHQ (HY-100489) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

N-acetyl-L-cysteine(NAC), hydrogen peroxide(H₂O₂), MTT [3(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and 4′,6-diamidno-2-phenylinole(DAPI) were purchased from the Sigma Chemical Co. (St. Louis, MO, USA). 2’,7’–dichlorofluorescin diacetate (DCF-DA) was purchased from Molecular Probe (Eugene, Oregon, USA). Rabbit antibodies which are reactive with Nrf2 (12,721) were from Cell Signaling Technology Inc. (Danvers, MA, USA). Mouse antibodies which are reactive with acetylated tubulin (T7451) or β-tubulin (T4026) were from Sigma-Aldrich Co. (St. Louis, MO, USA). Rabbit antibodies which are reactive with Arl13b (17,711–1-AP) were from Proteintech Group Inc. (Rosemont, IL, USA). Chicken anti-mouse IgG-Alexa 488 (A-21200) and goat anti-rabbit-Alexa 568 (A-11011) were obtained from Invitrogen (Calsbad, CA, USA). Except where indicated, all other materials are obtained from the Sigma Chemical Co. (St. Louis, MO, USA)^{17 (link)}.