Promoter cmv cytomegalovirus expression vector

Small interfering RNA (siRNA) of SNHG5 (si‐SNHG5), siRNA of NC, mir‐205‐5p mimics and NC were designed by RiboBio. si‐SNHG5 and mir‐205‐5p mimics were cloned into the promoter CMV (Cytomegalovirus) expression vector (Invitrogen) following the manufacturer's instructions, and then transferred to large artery smooth muscle cells. All SNHG5 knockout constructs were acquired from RiboBio. SNHG5 overexpression plasmid (pcdna‐SNHG5) was constructed by cloning the full‐length complementary DNA (cDNA) sequence of SNHG5 into pcDNA3.1 vector (Invitrogen). mir‐205‐5p overexpression plasmid (pcdna‐mir‐205‐5p) was obtained by cloning the full‐length cDNA sequence of mir‐205‐5p into pcDNA3.1 vector (Invitrogen). si‐mir‐205‐5p and SMAD4 mimics were cloned into promoter CMV (Cytomegalovirus) expression vector (Invitrogen) and transfected into arterial smooth muscle cells following the manufacturer's instructions.