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X60 1.42 na apo tirf oil immersion objective

Manufactured by Nikon
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The Nikon X60 1.42 NA APO TIRF oil-immersion objective is a high-numerical aperture (NA) objective designed for use in Total Internal Reflection Fluorescence (TIRF) microscopy. It has an NA of 1.42 and is an apochromatic (APO) corrected lens, providing high-resolution imaging capabilities.

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Lab products found in correlation

Elipse ti, by Nikon (2 mentions)

2 protocols using x60 1.42 na apo tirf oil immersion objective

1

Tracking Exocytosis in COS-1 Cells

Cited 1 time
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COS-1 cells were plated at low density on glass coverslips and transfected with APP-SEP (super ecliptic pHluorin) and with pARIS HTT or pARIS HTTSA (Pardo et al., 2010 (link)) using calcium phosphate. Acquisitions were made the day after transfection at 5 Hz during 1 min using an inverted microscope (Elipse Ti, Nikon) with a X60 1.42 NA APO TIRF oil-immersion objective (Nikon) coupled to a CCD camera (CoolSnap, Photometrics) and maintained at 37°C and 5% CO2. Analysis was done on area delimited by cell edges and exocytosis rate was quantified using ExocytosisAnalyser macro on ImageJ developed by Marine Scoazec.
Bruyère J., Abada Y.S., Vitet H., Fontaine G., Deloulme J.C., Cès A., Denarier E., Pernet-Gallay K., Andrieux A., Humbert S., Potier M.C., Delatour B, & Saudou F. (2020). Presynaptic APP levels and synaptic homeostasis are regulated by Akt phosphorylation of huntingtin. eLife, 9, e56371.
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2

Visualizing APP Exocytosis in COS-1 Cells

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COS-1 cells were plated at low density on glass coverslips and transfected with APP-SEP (super ecliptic pHluorin) and with pARIS HTT or pARIS HTT SA (Pardo et al., 2010) using calcium phosphate.
Acquisitions were made the day after transfection at 5Hz during 1 min using an inverted microscope (Elipse Ti, Nikon) with a X60 1.42 NA APO TIRF oil-immersion objective (Nikon) coupled to a CCD camera (CoolSnap, Photometrics) and maintained at 37°C and 5% CO2. Analysis was done on area delimited by cell edges and exocytosis rate was quantified using ExocytosisAnalyser macro on ImageJ developed by Marine Scoazec.
Bruyère J., Abada Y., Vitet H., Fontaine G., Deloulme J., Cès A., Denarier É., Pernet‐Gallay K., Andrieux A., Humbert S., Potier M., Delzescaux T., & Saudou F. (2020). Presynaptic APP levels and synaptic homeostasis are regulated by Akt phosphorylation of Huntingtin.
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