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Bca protein assay kit and protein ripa lysis buffer

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The BCA Protein Assay kit is a colorimetric assay used for the quantification of total protein concentration in a sample. The kit utilizes the bicinchoninic acid (BCA) reaction to detect and quantify the total protein present. The RIPA Lysis Buffer is a commonly used buffer solution designed to extract and solubilize proteins from cells and tissues for further analysis.

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Lab products found in correlation

Aevertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Faststart universal sybr green master and lightcycler96, by Roche (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit or anti mouse irdye 800 cw secondary antibody, by LI COR (2 mentions) Mouse anti rat tfr1, by Thermo Fisher Scientific (1 mentions) Rabbit polyclonal anti mouse fpn1, by Novus Biologicals (1 mentions) Il 6 enzyme linked immunosorbent assay elisa kits, by R&D Systems (1 mentions) Slc11a2, by Proteintech (1 mentions) Hif 2α, by Abcam (1 mentions)

Close spelling variants of this product

RIPA protein lysis buffer RIPA lysis buffer kit RIPA lysis buffer RIPA lysis kit RIPA assay buffer Protein lysis buffer BCA protein assay RIPA buffer Kit BCA protein kit Protein Lysis Kit

2 protocols using bca protein assay kit and protein ripa lysis buffer

1

Iron Homeostasis Regulation in Mouse Macrophages

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Unless otherwise stated, all chemicals were obtained from the Sigma Chemical Company, St. Louis, MO, United States. Mouse anti-rat TfR1 (transferrin receptor 1) and fetal bovine serum (FBS) were purchased from Invitrogen, Carlsbad, CA, United States; rabbit polyclonal anti-mouse Fpn1 from Novus Biologicals, Littleton, CO, United States; rabbit polyclonal anti-Ft-L and anti-DMT1 (divalent metal transporter 1, SLC11A2) from Protein-tech, Chicago, IL, United States; and rabbit polyclonal anti-phospho-STAT3 (Tyr705) and mouse monoclonal anti-STAT3 both from Cell Signaling Technology, Inc., Danvers, MA, United States. Recombined mouse IL-6 protein was obtained from Gene Script, Piscataway, NJ, United States; IL-6 enzyme-linked immunosorbent assay (ELISA) kits from R&D Systems, Minneapolis, MN, United States; goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibodies from LI-COR Bio Sciences, Lincoln, NE, United States; TRIzol reagent from Life Technologies, Carlsbad, CA, United States; AevertAid First Strand cDNA Synthesis Kit from Thermo Scientific, Waltham, MA, United States; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, United States; and BCA protein Assay kit and protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China.
Zhang F.L., Hou H.M., Yin Z.N., Chang L., Li F.M., Chen Y.J., Ke Y, & Qian Z.M. (2017). Impairment of Hepcidin Upregulation by Lipopolysaccharide in the Interleukin-6 Knockout Mouse Brain. Frontiers in Molecular Neuroscience, 10, 367.
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2

Molecular Mechanisms of Iron Regulation

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Unless otherwise stated, all chemicals were obtained from the Sigma Chemical Company, St. Louis, MO, USA. Mouse monoclonal anti-rat TfR1 was purchased from Invitrogen, Carlsbad, CA, USA; rabbit polyclonal anti-Fpn1 from Novus Biologicals, Littleton, CO, USA; mouse anti-p-JAK2 (phospho-Janus Kinase 2) from Cell Signaling Technology, Boston, USA; TRIzol reagent from Life Technologies, Carlsbad, CA, USA; and AevertAid First Strand cDNA Synthesis Kit from Thermo Scientific, Waltham, MA, USA. Rabbit polyclonal anti-Ft-Land anti-DMT1 were purchased from Protein-tech, Chicago, IL, USA; rabbit polyclonal anti-CBS, HIF-2α and EPO antibodies from Abcam, Inc., Cambridge, UK; rabbit polyclonal anti-Ft-H from Bioworld Technology Inc., Louis Park, MN, USA; goat anti-rabbit or anti-mouse IRDye 800 CW secondary antibody from LI-COR Bio Sciences, Lincoln, NE, USA; FastStart Universal SYBR Green Master and LightCycler96 from Roche, Nutley, NJ, USA; and BCA protein Assay kit and protein RIPA lysis buffer from Beyotime Institute of Biotechnology, Haimen, JS, China.
Zhao P., Qian C., Chen Y.J., Sheng Y., Ke Y, & Qian Z.M. (2019). Cystathionine β-synthase (CBS) deficiency suppresses erythropoiesis by disrupting expression of heme biosynthetic enzymes and transporter. Cell Death & Disease, 10(10), 708.
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