Coumarin 6

Polyvinyl alcohol (PVA) was purchased from Sigma‐Aldrich LLC [USA]. Ursolic acid, betulinic acid, glycyrrhetinic acid, and oleanolic acid was purchased from Nanjing Chunqiu Biological Products Co., Ltd [purity (98%), China]. Stigmasterol and β‐sitosterol was purchased from Aladdin reagent Co., Ltd [purity (98%), China]. Coumarin‐6, rhodamine 123 and rhodamine B200 was purchased from Shanghai Yuanye Bio‐Technology Co., Ltd [China]. The antibodies included the following: anti‐P Glycoprotein [GTX108354, GeneTex, USA]; anti‐Phospho‐p44/42 MAPK (p‐ERK1/2) (Thr202/Tyr204) [#4370S, Cell Signaling Technology (CST), USA] and anti‐beta Actin [GB15001, Servicebio, China].

Glycerin monostearate was purchased from Shanghai Maclin Biochemical Technology Co., Ltd. (Shanghai, China). Soya bean lecithin was obtained from Aiweituo Pharmaceutical Technology Co., Ltd. (Shanghai, China). Jing Wan Hong ointment was purchased from Tianjin DarentangJingwanhong Pharmaceutical Co., Ltd. (Tianjin, China). Neutral formalin was obtained from Beijing Yili Fine Chemicals Co., Ltd. (Beijing, China). Epidermal growth factor (EGF) antibody, basic fibroblast growth factor (bFGF) antibody, and coumarin 6 were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Chloralic hydras was obtained from the Tianjin Fuchen chemical reagent factory. Benzylpenicillin sodium for injection was purchased from Jiangxi Keda Animal Pharmaceutical Co., Ltd. (Fuzhou, China). Povidone iodine was obtained from Hunan Guangshengyuan Pharmaceutical Technology Co., Ltd. (Changsha, China). Veet depilatory cream was purchased from Reckitt Benckiser Household Products.

SA was purchased from CLKC Medicinal Chemistry Co., Ltd (Hubei, China), N-(3-dimethyl aminopropyl)-N-ethyl carbodiimide HCl (EDC), N-hydroxysuccinimide (NHS), dimethylformamide (DMF) were purchased from Macklin (Shanghai, China), chlorogenic acid was purchased from Qiaoyu Biotechnology Co., Ltd. (Shanghai, China), octadecylamine (ODA) was purchased from Feiyu Biotechnology Co., Ltd. (Nantong, China), hydrogenated soy phosphatidylcholine (HSPC), cholesterol (CH) were purchased from AVT Pharmaceutical Tech Co., Ltd. (Shanghai, China), anti-PD-1 (clone RMP1-14; 52.8 mg) was purchased from Bioxcell (West Lebanon, NH, USA), coumarin-6 was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide (DiR) was purchased from AAT Bioquest (State of California, USA), 4′,6-diamidino-2-phenylindole stain solution (DAPI), and 4% paraformaldehyde solution were purchased from Biosharp (Shanghai, China), dimethyl sulfoxide (DMSO) was purchased from VWR life science (PA, USA).

The paclitaxel was purchased from Wuxi Taxus Pharmaceutical Co., Ltd. (Jiangsu, China). Succinic anhydride (SA), 4-dimethyl aminopyridine (DMAP), 1-hydroxy-benzotriazole (HoBt), and 2-(1H-Benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Macklin (Shanghai, China). N,N-Diisopropylethylamine (DIPEA) was purchased from Aladdin (Shanghai, China). N-(2-Aminoethyl) maleimide trifluoroacetate salt (MAL) was purchased from J&K Chemical Ltd. (Beijing, China). TAR peptide (CRKKRRQRRRATWLPPR) was synthesized from China Peptide Co., Ltd. (Shanghai, China). Esterase and coumarin-6 were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China) Rabbit anti-NRP-1 antibody was purchased from Abcam (Cambridge, UK). Rabbit anti-β-actin antibody and secondary antibody (anti-rabbit) were purchased from ProteinTech (Beijing, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from BioFroxx (Einhausen, Germany). Cy5.5 maleimide (non-sulfonated, Cy5.5-MAL) was obtained from APExBIO (Houston, TX, USA). Tubulin-tracker green and DAPI were obtained from Beyotime (Shanghai, China). Regular agarose G-10 was purchased from Biowest (Spain). F-12K medium, heparin and endothelial cell growth supplement (ECGS) were obtained from MacGene (Beijing, China). 1640 medium was obtained from Meilunbio (Dalian, China).