Mkn 45 cl 0292

Two human GC cell lines, SNU-1 (CRL-5971) and AGS (CRL-1739) were procured from American Type Culture Collection (Manassas, VA, USA), and another two human GC cell lines, KATO III (CL-0372) and MKN-45 (CL-0292) from Procell Life Science & Technology Co., Ltd. (Wuhan, China). Human normal gastric epithelial cell line, GES-1 (SCSP-308), was procured from CCTCC (Shanghai, China). Roswell Park Memorial Institute-1640 medium encompassing 10% fetal bovine serum (FBS, Wisent, Montreal, Canada) and 1% antibiotics (100 U/mL penicillin G and 100 mg/mL streptomycin) was adopted for incubation of all aforesaid cell lines at 37ºC with 5% CO₂.
GenePharma (Shanghai, China) provided short hairpin RNA (sh)-SNHG22 1, 2, 3#, miR-361-3p mimic/inhibitor and their respective controls. The overexpression plasmid pcDNA-HMGA1 was designed and synthesized by GenePharma, and the empty PDNA3.1 vector served as the control. Then, cell transfection was conducted based on protocols of Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA).

The HGC-27 (CL-0107) and MKN-45 (CL-0292) cell lines of GC, obtained from Procell Life Science and Technology Co. (Wuhan, China), were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Procella, Wuhan, China) at 37 ℃ in a humid atmosphere consisting of 5% CO₂ and 95% air. RPMI-1640 contained 10% fetal bovine serum (FBS) (Procella, Wuhan, China) and 1% penicillin-streptomycin solution (Procell, Wuhan, China) as the medium.