Our primary variable of interest was log-transformed PRA. PRA was measured by radioimmunoassay using the GammaCoat Plasma Renin Activity Assay (DiaSorin Inc., Stillwater, MN, USA) and analysed in a central core laboratory (Quest Diagnostics, Valencia, CA USA). PRA was determined by quantification of angiotensin I in the presence of certain enzyme inhibitors. The dynamic range for this assay is 0.1 ng/mL/h to 50 ng/mL/h, intra-assay variation is 4.6–10%, and inter-assay variation is 5.6–7.6%. Laboratory personnel handling these samples were blinded to treatment assignment. All biomarker samples were obtained in the morning in a fasting state with patients in seated positions with their legs extended. To minimize the influence of outliers, the sponsor set the extremes of PRA recorded in the database to set values: <0.2 (set to 0.1) and >30 (set to 45). PRA was measured at the following time-points: baseline (time of randomization), 1 month, 6 months, 9 months, and 12 months.
Gammacoat plasma renin activity assay
Manufactured by DiaSorin
Sourced in United States
The GammaCoat Plasma Renin Activity Assay is a laboratory equipment product used to measure the activity of the enzyme renin in plasma samples. It is designed to provide accurate and reliable results for clinical diagnostic purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using gammacoat plasma renin activity assay
1
Plasma Renin Activity Measurement
2
Biomarker Analysis in Clinical Study
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood count was determined using ADVIA 120 or 2120 (Bayer Health Care, Tarrytown, NY, USA), and plasma sodium, potassium, creatinine, glucose, triglyceride, and total, high-density, and low-density lipoprotein cholesterol concentrations were determined using Cobas Integra 700/800 (F. Hoffmann-LaRoche Ltd, Basel, Switzerland). Since plasma creatinine was within the normal range, estimated glomerular filtration rate (GFR) was calculated with the RULE formula [19 (link)]. Plasma aldosterone was measured by radioimmunoassay (Aldosterone RIA Test DSL-8600, Diagnostics Systems Laboratories Inc, Webster, TX, USA) and renin activity utilising GammaCoat Plasma Renin Activity assay (Diasorin). Urinary tetrahydrocortisone (THE) and allo-tetrahydrocortisol plus tetrahydrocortisol (allo-THF+THF) concentrations were analysed using liquid chromatography-tandem mass spectrometry as described previously [20 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!