Torcar t a

Manufactured by Addgene

The TORCAR(T/A) is a laboratory equipment designed to perform automated cell culture tasks. It provides temperature and atmosphere control to maintain optimal conditions for cell growth and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

7gli gfp, by Addgene (2 mentions) Nf kb egfp, by Addgene (2 mentions) P38ktrmcerulean3, by Addgene (2 mentions) Jnkktrmruby2, by Addgene (2 mentions) 12xcsl d1egfp, by Addgene (2 mentions) Top gfp, by Addgene (1 mentions)

2 protocols using torcar t a

Multifaceted Signaling Pathway Profiling in Liver Cancer

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Wnt/β-catenin, Notch, Hedgehog, mTOR, NF-kB, P38, JNK, ERK and PKA reporter plasmids were overexpressed liver cancer cells, and treated with lactic acid or DM-αKG^{59 (link)}. The activity levels of each signaling pathway were detected by FACS. For example, Wnt activity level = (TOP-GFP intensity)/(FOP-GFP intensity). The plasmids used in this assay are: TOP-GFP (addgene no. 35489), FOP-GFP (addgene no. 35490), 12XCSL-d1EGFP (addgene no. 47684), 7Gli:GFP (addgene no.110494), TORCAR (addgene no. 64927), NF-kB-eGFP (addgene no. 118093), TORCAR(T/A) (addgene no. 64928), p38KTRmCerulean3 (addgene no. 59155), JNKKTRmRuby2 (addgene no. 59154), PKAKTRClover (addgene no.59153), ERKKTRClover (addgene no. 59150).

Chen Z., He Q., Lu T., Wu J., Shi G., He L., Zong H., Liu B, & Zhu P. (2023). mcPGK1-dependent mitochondrial import of PGK1 promotes metabolic reprogramming and self-renewal of liver TICs. Nature Communications, 14, 1121.

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Signaling Pathway Reporter Assay

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Signaling pathway (including Wnt/β-catenin, Notch, Hedgehog, mTOR, NF-kB, P38, JNK, ERK and PKA) reporter plasmids and control plasmids were overexpressed in HLM006474 treated and control cells, and the activity of signaling pathway can be detected by FACS. For example, Wnt activity= (TOP-GFP intensity)/(FOP-GFP intensity).
The plasmids used in this assay are: TOP-GFP (addgene no. 35489), FOP-GFP (addgene no. 35490), 12XCSL-d1EGFP (addgene no. 47684), 7Gli:GFP (addgene no.110494), TORCAR (addgene no. 64927), TORCAR(T/A) (addgene no. 64928), NF-kB-eGFP (addgene no. 118093), p38KTRmCerulean3 (addgene no. 59155), JNKKTRmRuby2 (addgene no. 59154), ERKKTRClover (addgene no. 59150), PKAKTRClover (addgene no.59153).

Chen Z., Liu B., Huang L., Zhong X., Yan Z., & Zhu P. (2021). rtcisE2F drives liver TIC self-renewal and metastasis via m⁶A-modulated mRNA stability of E2F6 and E2F3.

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