L deprenyl hcl

Manufactured by Merck Group

Sourced in United States

L-deprenyl·HCl is a chemical compound used in research laboratories. It is the hydrochloride salt of the selective and irreversible monoamine oxidase-B (MAO-B) inhibitor L-deprenyl. This compound is utilized for its ability to inhibit the MAO-B enzyme, which plays a role in the metabolism of certain neurotransmitters. The core function of L-deprenyl·HCl is to serve as a research tool in studies investigating the effects of MAO-B inhibition.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Deprenyl (13 citations)

4 protocols using l deprenyl hcl

Kurarinone Isolation and Enzymatic Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kurarinone
was isolated from the ethyl acetate fraction of S.
flavescens radix as reported in our earlier work.^{16 (link)} Recombinant Chinese hamster ovary (CHO) and
human embryonic kidney (HEK-293) cells were generated by Eurofins
Discovery (Le Bois I′ Eveque, France). ThermoFisher Scientific
(Madison, U.S.A.) provided cell-culture media such as Roswell Park
Memorial Institute (RPMI-1641), Dulbecco’s modified Eagle medium
buffer, Hank’s balanced salt solution (HBSS) buffer, and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic
acid (HEPES) buffer. The MAO-A/B assay kit was acquired from Promega
(Promega Cooperation, Madison, WI). The recombinant hMAO isoenzymes
and the reference drugs: l-deprenyl·HCl, DA, 5-HT, AVP,
clozapine, (+) butaclamol, SCH 23390, (S)-WAY-100635, and [d(CH₂)₅¹, Tyr(Me)₂]-AVP were supplied
by Sigma-Aldrich (St. Louis, MO, USA). The remaining chemicals of
reagent grade were procured from commercial suppliers.

Prajapati R., Seong S.H., Paudel P., Park S.E., Jung H.A, & Choi J.S. (2021). In Vitro and In Silico Characterization of Kurarinone as a Dopamine D1A Receptor Antagonist and D2L and D4 Receptor Agonist. ACS Omega, 6(49), 33443-33453.

+ Open protocol

+ Expand

Dopaminergic Neurotransmission Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell culture reagents except fetal bovine serum (FBS, Cambrex IEP GmbH (Wiesbaden, Germany)) were obtained from Gibco, Invitrogen (Paisley, UK). [³H]-dopamine (2220 GBq/mmol) was purchased from Perkin Elmer (Hopkinton, MA, USA), an E.Z.N.A.^® Total RNA Kit I from Omega Bio-Tek (Norcross, GA, USA), and a High Capacity cDNA Reverse Transcription Kit, TaqMan Gene Expression Assays and TaqMan^® Universal PCR Master Mix from Applied Biosystems (Carlsbad, CA, USA). Decynium-22 (D22), nortriptyline HCl, apomorphine HCl, haloperidol, L-DOPA, L-deprenyl HCl and tropolone were obtained from Sigma Aldrich (St. Louis, MO, USA), and desipramine HCl was obtained from Sandoz (Cham, Switzerland). GBR12909 was obtained from Tocris (Bristol, UK).

Sočan V., Dolinar K, & Kržan M. (2024). Kinetic Properties and Pharmacological Modulation of High- and Low-Affinity Dopamine Transport in Striatal Astrocytes of Adult Rats. International Journal of Molecular Sciences, 25(10), 5135.

+ Open protocol

+ Expand

Tanshinone Isomers Isolation and Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tanshinone I (1), tanshinone IIA (2), and cryptotanshinone (3) of >98% purity were obtained from the dried roots of Salvia miltiorrhiza, as described in our published report [29 (link)]. The chemical structures of 1, 2, and 3 are shown in Figure 1. Recombinant hMAO-A and hMAO-B, l-deprenyl·HCl, clorgyline·HCl, 3-isobutyl-1-methylxanthine (IBMX), and acetylcholine chloride (ACh) were purchased from Sigma Aldrich (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium (DMEM), Hank’s balanced salt solution (HBSS), and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were bought from Invitrogen (Waltham, MA, USA). The remaining reagents and chemicals were purchased from commercial suppliers.

Prajapati R., Park S.E., Seong S.H., Paudel P., Fauzi F.M., Jung H.A, & Choi J.S. (2021). Monoamine Oxidase Inhibition by Major Tanshinones from Salvia miltiorrhiza and Selective Muscarinic Acetylcholine M4 Receptor Antagonism by Tanshinone I. Biomolecules, 11(7), 1001.

+ Open protocol

+ Expand

Recombinant hMAO Inhibition and GPCR Functional Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the recombinant hMAO inhibition assay, a MAO-A/B assay kit (Promega Cooperation, Madison, WI, USA), recombinant hMAO isoenzymes, and the standards l-deprenyl⋅HCl (Sigma Aldrich, St. Louis, MO, USA) were used. For the GPCR functional assay, reference drugs of DA, serotonin, AVP, clozapine, butaclamol, SCH23390, (S)-WAY-100635 and [d(CH₂)₅¹, Tyr(Me)₂]-AVP as well as the test compound, ILQ, were purchased from Sigma-Aldrich (St. Louis, MO, USA). Transfected CHO and human embryonic kidney (HEK-293) cell lines were generated by Eurofins Discovery (Le Bois I’Eveque, France). The cell culture media of Roswell Park Memorial Institute (RPMI-1641), Dulbecco's modified Eagle medium (DMEM) buffer, Hank’s balanced salt solution (HBSS) buffer, and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer were procured from ThermoFisher Scientific (USA).

Prajapati R., Seong S.H., Park S.E., Paudel P., Jung H.A, & Choi J.S. (2021). Isoliquiritigenin, a potent human monoamine oxidase inhibitor, modulates dopamine D1, D3, and vasopressin V1A receptors. Scientific Reports, 11, 23528.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!