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Mutagenesis reagents

Manufactured by Agilent Technologies
Sourced in United States

Mutagenesis reagents are a set of specialized chemicals used in molecular biology laboratories to induce controlled genetic mutations in DNA sequences. These reagents facilitate the process of creating genetic variations, which is essential for various research applications, such as gene function studies and directed evolution experiments.

Automatically generated - may contain errors

2 protocols using mutagenesis reagents

1

Yeast Cell Culture and Protein Purification

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Yeast DOB were obtained in powder form from MP Biomedicals (Solon, OH, USA). Cell culture plastics were from BD Falcon (San Jose, CA, USA). Dextrose, galactose, and raffinose were obtained from Sigma (St. Louis MO, USA). Glass beads for yeast cell lysis were from B. Braun Biotech (Allentown, PA, USA). Anti-V5-HRP antibody was from Invitrogen (Carlsbad, CA, USA). Mutagenesis reagents were obtained from Agilent (Santa Clara, CA, USA). All other chemicals were reagent grade or better, were purchased from Sigma (St. Louis MO, USA) and used without additional purification.
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2

Yeast Cell Lysis and Protein Expression

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Yeast DOB were obtained in powder form from MP Biomedicals (Solon, OH). Cell culture plastics were from BD Falcon. Glucose, galactose, and raffinose were obtained from Sigma (St. Louis, MO). Glass beads for yeast cell lysis were from B. Braun Biotech (Allentown, PA). Anti-V5-HRP antibody was from Invitrogen (Carlsbad, CA). Mutagenesis reagents were obtained from Agilent (Santa Clara, CA). All other chemicals were reagent grade or better and were purchased from Sigma and used without additional purification.
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