L α phosphatidylcholine from egg yolk eggpc

α-N-Hydroxysuccinimidyl-ω-Boc protected-amino-PEG (NHS-PEG-NH₂-Boc, Mw: 5000), 1,2-dimyristoyl-sn-glycerol-3-phosphatidylethanolamine (DMPE), 1,2-dipalmitoyl-sn-glycerol-3-phosphatidylethanolamine (DPPE), and 1,2-distearoyl-sn-glycerol-3-phosphatidylethanolamine (DSPE) were purchased from NOF Corporation (Tokyo, Japan). Dichloromethane, chloroform, toluene, diethyl ether, and n-butyl amine were obtained from Nacalai Tesque (Kyoto, Japan). Methoxyl-PEG-succinimidyl propionate (MeO-PEG-NHS, Mw 5000) was purchased from Nektar Therapeutics (San Carlos, CA, USA). L-α-Phosphatidylcholine from egg yolk (EggPC) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Fluorescein isothiocyanate (FITC) was obtained from Dojindo Laboratories (Kumamoto, Japan). Phosphate-buffered saline (PBS) was obtained from Nissui Pharmaceutical, Co., Ltd (Tokyo, Japan). 1-Hexadecanethiol was purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Trifluoroacetic acid (TFA) and phospholipid C-test wako were purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Octadecyl triethoxysilane was supplied from Shin-Etsu Chemical Co., Ltd (Tokyo, Japan). L-α-Palmitoyl-NBD dodecanoyl phosphatidylethanolamine was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA).

1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), distearoylphosphatidylethanolamine (DSPE)-mPEG₂₀₀₀, and DSPE-PEG₂₀₀₀-folate were purchased from Avanti Polar Lipid (Alabaster, AL, USA). l-α-Phosphatidylcholine from egg yolk (Egg PC) and HEPES were obtained from Sigma-Aldrich (St. Louis, MO, USA). Magnetic nanoparticles (15 nm) were purchased from Ocean Nanotech (San Diego, California, USA). NHS-PEG₃₄₀₀-maleimide was obtained from Laysan Bio (Arab, AL, USA). N-Succinimidyl S-acetylthioacetate was purchased from Thermo Scientific (Waltham, MA, USA). The anti-HER2 antibody was purchased from Roche (Basel, Switzerland). All other chemicals and solvents were purchased from Sigma-Aldrich and were of ACS reagent grade or biotechnology grade.