Albumin from human serum (HSA; lyophilized powder, free of fatty acid and globulin, ≥99% pure, via agarose gel electrophoresis) was purchased from Sigma (St. Louis, MO, USA). The Pierce bicinchoninic acid (BCA) protein assay kit was purchased from ThermoScientific. Radio-labeled zinc was purchased as 65ZnCl2 from PerkinElmer with a radionuclide purity of 99.0% (half-life = 244 days) and diluted in distilled and deionized H2O (DDW, 18 MΩ) to prepare a stock solution whose concentration of 65Zn2+ was calculated daily.
Albumin from human serum hsa
Manufactured by Merck Group
Sourced in United States
Albumin from human serum (HSA) is a laboratory product derived from human blood plasma. It serves as a key component in various biochemical and cell culture applications, primarily due to its ability to stabilize and maintain the integrity of biological samples and solutions.
Automatically generated - may contain errors
Lab products found in correlation
Absolute ethanol, by Avantor (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Pierce bicinchoninic acid bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Lysis buffer, by BD (1 mentions)
Picoline borane, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 cell culture media, by Merck Group (1 mentions)
Ethanolamine, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Methylcellulose, by Merck Group (1 mentions)
Pepsin from hog stomach, by Merck Group (1 mentions)
Sodium triphosphate, by Merck Group (1 mentions)
Bovine serum bsa, by Merck Group (1 mentions)
Barnstead micropure system, by Thermo Fisher Scientific (1 mentions)
α amylase, by Merck Group (1 mentions)
Sodium chloride (nacl), by Carl Roth (1 mentions)
Potassium chloride (kcl), by Avantor (1 mentions)
Potassium hydroxide, by Carl Roth (1 mentions)
Nitric acid, by Carl Roth (1 mentions)
Ferritin from equine spleen, by Merck Group (1 mentions)
5 protocols using albumin from human serum hsa
1
Radiolabeled Zinc Binding Assay
2
Isolation and Labeling of Circulating Tumor Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Albumin from human serum (HSA), ethanolamine, picoline borane, Hoechts 33342 dye, RPMI 1640 cell culture media, and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St Louis, MO, USA). Fetal bovine serum, fluorescein isothiocyanate (FITC) labeled anti-EpCAM and 1% Penicillin-Streptomycin were from Thermo Fisher Scientific (Waltham, MA, USA). Lysis buffer was purchased from Becton Dickinson (Franklin Lakes, NJ, USA). The glutaraldehyde was from Carl Roth Chemicals (Karlsruhe, Germany) and the absolute ethanol from VWR (Radnor, PA, USA). Human immunoglobulin G1 was obtained from Molecular Innovations (Novi, MI, USA).
3
Hybrid Biomaterial Synthesis and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Methylcellulose (viscosity: 15 cP), sodium humate (Mw 226.14 g/mol), cisplatin (Mw 300.05 g/mol), phosphate-buffered saline (PBS, tablet, pH 7.4), human hemoglobulin (HB), and Albumin from human serum (HSA) were purchased from Sigma-Aldrich Co. (Missouri, United States). (3-Glycidyloxypropyl)trimethoxysilane (GPTMS, Mw 236.34 g/mol) and sodium hydroxide (Mw 40 g/mol) were purchased from Rhawn Co. (Shanghai, China). All chemicals were utilized directly without further purification. Aqueous solutions were prepared with deionized (DI) water.
4
Synthesis and Evaluation of Hexahydrocurcumin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chitosan (CS, 75 kDa, 84.3 ± 0.2% deacetylated) was provided by Marine Bio-Resources Co., Ltd. (Samut Sakhon, Thailand). Sodium triphosphate (TPP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Pluronic® F-127mucin powder from porcine stomach Type III, α-amylase, porcine lipase Type II and bile extract powder, pepsin from hog stomach, pancreatin from hog pancreas, and albumin from human serum (HSA) and bovine serum (BSA) were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). Fetal bovine serum (FBS) was obtained from Life Science Production (LPS, Bedford, UK). Serum-free Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Invitrogen Cop. (Grand Island, NY, USA). Hexahydrocurcumin (HHC) was synthesized from curcumin (Cur) following the method described by Srimuangwong et al. [15 (link)]. Wright-Giemsa dye was purchased from M&P IMPEX Ltd. (Ladkrabang, BKK, Thailand). Analytical grades of acetic acid, acetone, absolute ethanol, hydrogen peroxide, and dimethyl sulfoxide (DMSO) were purchased from Carlo Erba reagents (Val de Reuil, France). Ultrapure water was obtained from the Thermo Scientific Barnstead MicroPure system (Thermo Fisher Scientific Inc., Waltham, MA, USA). Other reagents were used without further purification.
5
Protocols for Protein Binding Experiments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Scopoletin (7-Hydroxy-6-methoxycoumarin), sodium dodecyl sulfate (SDS), hydrogen peroxide and (11-mercaptoundecyl)tetra-(ethylene glycol) were purchased from Sigma Aldrich (Steinheim, Germany). Absolute ethanol (≥ 99.8 %), hydrochloric acid, potassium chloride and sodium hydroxide from VWR (Leuven, Belgium); dipotassium phosphate, potassium dihydrogen phosphate, potassium hexacyanoferrate (II) trihydrate and ammonia solution 25 % from Merck (Darmstadt, Germany); sulfuric acid (95 -98 %), nitric acid (≥ 65 %), sodium chloride, potassium hexacyanoferrate (III) and potassium hydroxide from Roth (Karlsruhe, Germany); disodium hydrogen phosphate dihydrate and Tris from Duchefa Biochemie (Haarlem, the Netherlands); the proteins holo-Trf, apo-Trf from bovine, albumin from human serum (HSA), ferritin from equine spleen, urease from jack beans, proteinase K from Engyodontium album and cytochrome c (cyt c) from equine heart were purchased from Sigma Aldrich. AT-cut QCM crystals (5 MHz) were purchased from Q-sense (Q-Sense AB, Sweden). All solutions were prepared with deionized water (DI water) obtained from a water purification system (Millipore, Eschborn, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!