Sodium hydrosulfide (NaHS) was purchased from Sigma-Aldrich (Merck KGaA; Darmstadt, Germany; cat. no. 161527). LY294002 (a PI3K inhibitor) was purchased from ApexBio (Houston, TX, USA; cat. no. 8250–200 mg). Akt, phospho (p)-Akt and caspase-3 antibodies were obtained from Abcam (Cambridge, MA, USA; cat. nos. ab179463, ab192623 and ab13847, respectively). PI3K, p-PI3K and nuclear factor-κB (NF-κB) antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA; cat. nos. 4249, 4228 and 8242, respectively). B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) antibodies were obtained from Boster Biological Technology, Ltd. (Wuhan, China; cat. nos. BA0412 and BA0315-2, respectively). Peroxidase-conjugated AffiniPure goat anti-rabbit immunoglobulin G (IgG), peroxidase-conjugated AffiniPure goat anti-mouse IgG and mouse anti-β-actin monoclonal antibodies were obtained from OriGene Technologies, Inc. (Beijing, China; cat. nos. ZB-2301, ZB-2305 and TA-09, respectively). In situ Cell Death Detection kit, POD was obtained from Roche Diagnostics GmbH (Mannheim, Germany; cat. no. 11684817910). Rat troponin I (TnI), interleukin (IL)-10, IL-6 and tumor necrosis factor-α (TNF-α) ELISA kits were provided by Elabscience Biotechnology Co., Ltd. (Wuhan, China; cat. nos. E-EL-R0055c, E-EL-R0016c, E-EL-R0015c and E-EL-R0019c, respectively).
Sodium hydrosulfide nahs
Manufactured by Merck Group
Sourced in United States, Germany
Sodium hydrosulfide (NaHS) is a chemical compound that is commonly used in laboratory settings. It is a white or pale yellow crystalline solid with the chemical formula NaHS. The primary function of sodium hydrosulfide is to serve as a source of sulfide ions (S2-) in various chemical reactions and analytical procedures.
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Lab products found in correlation
Bicinchoninic acid bca protein assay kit, by Beyotime (3 mentions)
Malondialdehyde mda assay kit, by USCN (2 mentions)
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Nuclear factor κb nf κb, by Cell Signaling Technology (1 mentions)
Ly294002, by Apexbio (1 mentions)
Tnf α elisa kit, by Elabscience (1 mentions)
Hoechst staining kit, by Beyotime (1 mentions)
Oil red o, by Merck Group (1 mentions)
Ultrapure escherichia coli 0111 b4 lps free of lipoproteins, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Total sod assay kit, by Beyotime (1 mentions)
Sirt1 antibody, by Abcam (1 mentions)
Goat anti rat antibody, by Proteintech (1 mentions)
β actin antibody, by Proteintech (1 mentions)
Superoxide dismutase sod assay kit, by USCN (1 mentions)
Glutathione gsh enzyme linked immunosorbentassay elisa kits, by USCN (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Aldh2 activity assay kit, by Abcam (1 mentions)
Anti aldh2 antibody, by Abcam (1 mentions)
Hydrogen peroxide assay kit, by Beyotime (1 mentions)
18 protocols using sodium hydrosulfide nahs
1
Cardioprotective Role of NaHS in Ischemia
2
Regulation of PCSK9, LDLR and Lipid Metabolism
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Sodium hydrosulfide (NaHS) and Oil Red O were purchased from Sigma-Aldrich (St. Louis, MO, USA). Specific monoclonal anti-PCSK9 (cat. no. 55206), anti-LDLR (cat. no. 66414), anti-SREBP-2 (cat. no. 557037) and anti-SREBP-1-c (cat. no. 66875) antibodies were purchased from Proteintech (Rosemont, IL, USA). Specific monoclonal anti-protein kinase B (Akt) antibody (cat. no. BM4400) was purchased from Wuhan Boshide Bioengineering Co. (Wuhan, China). Specific monoclonal anti-phosphorylated Akt antibody (cat. no. YM3621) was purchased from ImmunoWay Biotechnology (Plano, TX, USA). Anti-β-actin antibody (cat. no. LCA01) was purchased from Aijia Biological Technology Co., Ltd. (Changsha, China). Cy3-conjugated goat anti-rabbit IgG secondary antibody (cat. no. E031620) was purchased from EarthOx Life Sciences (Millbrae, CA, USA). Dulbecco's modified Eagle's medium (DMEM) was purchased from HyClone (Logan, UT, USA). Fetal bovine serum (FBS) was purchased from Hangzhou Sijiqing Biocompany (Hangzhou, China). The Hoechst Staining kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate-labeled LDL (DiI-LDL) was purchased from Haoyuan Biological Technology Co., Ltd. (Guangzhou, China). siRNAs were synthesized by Ruibo Biotechnology Co., Ltd. (Guangzhou, China).
3
Hydrogen Sulfide Modulates LPS-Induced Inflammation
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Tamm-Horsfall protein 1 (THP-1) cells were maintained in RPMI-1640 supplemented with 2 mm l-glutamine, 100 U/ml penicillin, 100 μg/ml streptomycin and 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA). Ultrapure Escherichia coli 0111:B4 LPS free of lipoproteins was obtained from Invitrogen (San Diego, CA, USA). The cells were plated in 22-mm tissue culture dishes (2×106 cells/dish). Macrophage differentiation was induced with phorbol myristate acetate (PMA, 100 nM) for 5 h. In one set of experiments (pre-treatment experiments) the effects of H2S were examined following a 30-min pre-treatment with sodium hydrosulfide (NaHS, an H2S donor) (Sigma) at 0.01, 0.1, 0.5 or 1 mM followed by a washout, followed by incubation with bacterial lipopolysaccharide (LPS, 1 μg/ml) in 1% FSB RPMI-1640 for 1, 4, 8 or 24 h. In another set of experiments (co-treatment experiments), NaHS was administered 30 min prior to the LPS administration, without a washout. The control cells were maintained in 1% FBS RPMI-1640.
4
Sodium Hydrosulfide Modulates Oxidative Stress
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Sodium hydrosulfide (NaHS, a donor of H2S) was purchased from Sigma (Sigma, St. Louis, MO, USA). Pelltobarbitalum Natricum was obtained from Germany and the Sirt1-antibody was purchase from American Abcam. The primary antibodies of CPR78, CHOP, cleaved caspase-12, Bax and Bcl2 were bought from cell signaling technolgy. The malondialdehyde (MDA) assay kit was bought from Uscn Life Science Inc. (Wuhan, Hubei, China). The glutathione (GSH) enzyme-linked immunosorbent assay (ELISA) kit was purchased from Bio-Swamp Life Science. Total SOD assay kit and Bicinchoninic Acid (BCA) Protein Assay Kit were obtained from Beyotime Institute of Biotechnology (Shanghai, China). Specific monoclonal antibody for detecting Sirtinol was purchased from Santa. Bicinchoninic Acid (BCA) Protein Assay Kit was obtained from Beyotime Institute of Biotechnology (Shanghai, China).
5
Sodium Hydrosulfide Cell Treatment
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Sodium hydrosulfide (NaHS) obtained from (Sigma-Aldrich, Darmstadt, Hesse, Germany) was freshly dissolved in millipore water shortly before cell treatment.
6
Molecular Mechanisms of Hydrogen Sulfide
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Sodium hydrosulfide (NaHS, a donor of H2S) and K252a (a selective pharmacological p-TrkB inhibitor) were purchased from Sigma (Sigma, St. Louis, MO, USA). Specific monoclonal antibodies for detecting BDNF and p-TrkB were obtained from Epitomic Inc. (Burlingame, UK). β-actin antibody and goat anti-rat antibody were purchased from Proteintech (Danvers, MA, USA). The 4-hydroxy-2-trans-nonenal (4-HNE) assay kit, malondialdehyde (MDA) assay kit, superoxide dismutase (SOD) assay kit, and glutathione (GSH) enzyme-linked immunosorbent assay (ELISA) kits were bought from USCN Life Science Inc. (Wuhan, Hubei, China). Bicinchoninic Acid (BCA) Protein Assay Kit was obtained from Beyotime Institute of Biotechnology (Shanghai, China).
7
Hydrogen Sulfide Signaling Modulation
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Sodium hydrosulfide (NaHS, a donor of H2S) was obtained from Sigma (Sigma, USA). Acrp30-antibody was purchased from Santa Cruz Biotechnology (California, USA). The primary antibodies of synapsin1, beclin1, and p62/SQSTMI were purchased from Cell Signaling Technology. Bicinchoninic Acid (BCA) Protein Assay Kit was purchased from Beyotime Institute of Biotechnology in Shanghai.
8
Hcy-Induced ALDH2 Modulation and Oxidative Stress
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Sodium hydrosulfide (NaHS, a donor of H2S) and Hcy were purchased from Sigma Chemical. Anti-ALDH2 antibody was purchased from Abcam Technology. ALDH2 activity assay kit was obtained from Abcam. MDA enzyme linked immunosorbent assay (ELISA) kit was purchased from Uscn Life Science. 4-HNE and GSH ELISA kits were obtained from Bio-Swamp Life Science.
9
Cultivation and Genetic Manipulation of Roseobacter Strains
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R. pomeroyi DSS-3, R. denitrificans, and R. lacuscaerulensis are gifts from Prof. Yuzhong Zhang of the Ocean University of China. R. pomeroyi DSS-3 derivatives ∆pdo and ∆sqr∆fccAB were constructed previously in our lab (48 (link)). R. pomeroyi DSS-3 ∆mtoX was constructed using previously reported methods (49 (link), 50 (link)). Details of the construction method are provided in the supplemental material. E. coli strains used for plasmid construction and protein expression, plasmids constructed in this study are all listed in Table S1. R. pomeroyi DSS-3 strain was cultured in the 1/2 YTSS medium, which contains 4 g/L yeast extract, 2.5 g/L tryptone, and 20 g/L sea salts. For cultivation, R. pomeroyi DSS-3 strains were cultivated at 30°C with shaking (220 rpm). E. coli strains were cultured in lysogeny broth (LB) medium at 37°C.
Sodium hydrosulfide (NaHS) was purchased from Sigma-Aldrich (Saint Louis, MO). Hydrogen Peroxide Assay Kit was purchased from Beyotime Biotechnology (Shanghai, China). MT was purchased from Macklin Biochemical Co., Ltd (Shanghai, China), and ADHP and horseradish peroxidase (HRP) enzymes were purchased from Aladdin Biotech (Shanghai, China).
Sodium hydrosulfide (NaHS) was purchased from Sigma-Aldrich (Saint Louis, MO). Hydrogen Peroxide Assay Kit was purchased from Beyotime Biotechnology (Shanghai, China). MT was purchased from Macklin Biochemical Co., Ltd (Shanghai, China), and ADHP and horseradish peroxidase (HRP) enzymes were purchased from Aladdin Biotech (Shanghai, China).
10
Diabetic Kidney Disease Model
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Glycine, Tris, SDS, mannitol, NaCl, bovine serum albumin (BSA), STZ and sodium hydrosulfide (NaHS) were purchased from Sigma-Aldrich (St Louis, MO. USA). Bradford colorimetric protein assay kit (Rockford, IL.USA) was used for protein quantification. The RIPA buffer was from Thermo Fisher Scientific Inc (Waltham, MA. USA). The phosphorus, calcium and alkaline phosphatase (ALP) kits were purchased from Jiancheng Bioengineering Co (Nanjing, China). High fat diet with 0.75% adenine was from TROPHIC Animal Feed High-tech Co Ltd (Nantong, China). Other chemicals and reagents were of analytical grade.
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