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Ov100 small animal imaging system

Manufactured by Olympus
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Sourced in Japan, Germany

The OV100 Small Animal Imaging System is a compact and versatile imaging platform designed for in vivo visualization of biological processes in small animal models. The system integrates high-resolution optics, sensitive cameras, and advanced imaging technologies to capture detailed images and videos of various fluorescent and bioluminescent markers within living subjects. The OV100 is capable of providing real-time, non-invasive monitoring of disease progression, therapeutic responses, and other biological phenomena in a wide range of research applications.

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Lab products found in correlation

Mt 20 light source, by Olympus (13 mentions) Dp70 ccd camera, by Olympus (12 mentions) Fv1000 confocal laser scanning microscope, by Olympus (5 mentions) Mvx10 long working distance microscope, by Olympus (2 mentions) Fv1000 confocal microscope, by Olympus (2 mentions) Matrigel, by BD (2 mentions) Ov100, by Olympus (2 mentions) Fluoview fv10 asw software, by Olympus (2 mentions) Iv100, by Olympus (2 mentions) Fv1000, by Olympus (2 mentions) Ef s18 55 is lens, by Canon (1 mentions) Eos 60d digital camera, by Canon (1 mentions) Iv100 laser scanning microscope, by Olympus (1 mentions) Female athymic nude mice, by Charles River Laboratories (1 mentions) Imaging pro plus 6, by Media Cybernetics (1 mentions) Vi cell xr, by Beckman Coulter (1 mentions) Phosphate buffered saline (pbs), by Corning (1 mentions) Fv1000 confocal laser microscope, by Olympus (1 mentions) Fluoview software, by Olympus (1 mentions) Dp70 charge coupled device camera, by Olympus (1 mentions)

49 protocols using ov100 small animal imaging system

1

Intravital Imaging of Tumor Growth

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Mice were intravitally imaged for GFP expression weekly using the Olympus OV100 small animal imaging system (Olympus Corp. Tokyo, Japan), containing an MT-20 light source (Olympus Biosystems Planegg, Germany) and DP70 CCD camera (Olympus Corp. Tokyo, Japan) (22 (link)). Images were used to calculate tumor area with Image-J (National Institute of Health Bethesda, MD) and were processed with the use of Photoshop elements-11 (Adobe Systems Inc. San Jose, CA). Tumor area has previously been shown to correlate with tumor volume (23 (link)).
Maawy A.A., Hiroshima Y., Zhang Y., Garcia-Guzman M., Luiken G.A., Kobayashi H., Hoffman R.M, & Bouvet M. (2015). Photoimmunotherapy lowers recurrence after pancreatic cancer surgery in orthotopic nude mouse models. The Journal of surgical research, 197(1), 5-11.
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2

In Vivo Small Animal Imaging Protocols

Cited 3 times
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The Olympus OV100 Small Animal Imaging System (Olympus Corp., Tokyo, Japan), containing an MT-20 light source (Olympus Biosystems, Planegg, Germany) and DP70 CCD camera (Olympus Corp., Tokyo, Japan) [36 (link)]; the Dino-Lite imaging system (AM4113T-GFBW Dino-Lite Premier; AnMo Electronics Corporation, Taiwan) [30 (link)]; and the MVX10 long-working distance microscope (Olympus Corp.) [37 (link)] were used for imaging live mice. To analyze for recurrence and to follow tumor progression postoperatively, weekly noninvasive whole-body imaging of the mice was performed using the iBox Scientia Small Animal Imaging System (UVP LLC, Upland, CA, USA). GFP fluorescent-tumor areas were recorded every week [16 (link), 38 (link)–40 (link)]. The working-distance setting for GFP imaging in the iBox was adjusted to image the maximum possible fluorescent area in each mouse both before surgery and after surgery. All images were analyzed with ImageJ v1.440 (National Institutes of Health).
Uehara F., Hiroshima Y., Miwa S., Tome Y., Yano S., Yamamoto M., Matsumoto Y., Maehara H., Tanaka K., Bouvet M., Kanaya F, & Hoffman R.M. (2015). Fluorescence-Guided Surgery of Retroperitoneal-Implanted Human Fibrosarcoma in Nude Mice Delays or Eliminates Tumor Recurrence and Increases Survival Compared to Bright-Light Surgery. PLoS ONE, 10(2), e0116865.
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3

Fluorescence-Guided Osteosarcoma Resection

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A total of 32 mice were used (Fig. 1): 16 mice underwent FGS, and the other 16 mice underwent bright-light surgery (BLS). Two weeks after the implantation of the cancer cells, tumor-bearing mice were randomly assigned to the BLS or FGS group. Before resection of the osteosarcoma, mice were anesthetized by ketamine mixture, and their limbs were sterilized with alcohol. An approximately 1.5 cm incision was made above the tumor. Resection of the primary osteosarcoma was performed using the MVX-10 long-working distance, high-numerical aperature microscope (Olympus Corp, Tokyo, Japan) under bright-light illumination for the BLS-treated mice and under fluorescence illumination through an RFP filter (excitation HQ 545/30x; emission 620/60m) for the FGS-treated mice. For osteosarcoma resection, intralesional and marginal tumor excision was performed in all the mice. Postoperatively, the surgical resection bed was imaged with the Olympus OV-100 Small Animal Imaging System (Olympus Corp) under both standard bright field and fluorescence illumination to assess the completeness of surgical resection.
Miwa S., Hiroshima Y., Yano S., Zhang Y., Matsumoto Y., Uehara F., Yamamoto M., Kimura H., Hayashi K., Tsuchiya H, & Hoffman R.M. (2014). Fluorescence-Guided Surgery Improves Outcome in an Orthotopic Osteosarcoma Nude-Mouse Model. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 32(12), 1596-1601.
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4

Fluorescent Antibody Imaging of Tumor Models

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In both subcutaneous and orthotopic tumor models, the mice were injected with the fluorescent antibody in the tail vein. After 48 hours, whole body non-invasive and intra-vital imaging of the subcutaneous and orthotopic tumors was performed using the OV100 Small Animal Imaging System (Olympus, Tokyo, Japan) [41 (link)]. The optimal dose for animal studies was decided by the amount of the conjugated antibody which produced images with the best TBR in the subcutaneous-tumor model.
Park J.Y., Lee J.Y., Zhang Y., Hoffman R.M, & Bouvet M. (2016). Targeting the insulin growth factor-1 receptor with fluorescent antibodies enables high resolution imaging of human pancreatic cancer in orthotopic mouse models. Oncotarget, 7(14), 18262-18268.
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5

Combination Therapy for Tumor Regression

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Mice were randomized to 4 groups and treated as follows: (G1) saline (vehicle/control, ip, weekly, 4 weeks); (G2) GEM (80 mg/kg, ip, weekly, 4 weeks); (G3) BEV (5 mg/kg, ip, twice a week, 4 weeks) / GEM (Eli Lilly, Indianapolis, IN) (80 mg/kg, ip, weekly, 4 weeks) and (G4) BEV (Genentech, South San Francisco, CA) (5 mg/kg, ip, twice a week, 2 weeks) / GEM (80 mg/kg, ip, weekly, 2 weeks) → S. typhimurium A1-R (5 × 107 CFU/body, iv, weekly, 2 weeks) (Fig. 4A). Each treatment group comprised 5 tumor-bearing mice. Body weights of the mice were determined on a balance once a week. Tumor size was measured with calipers in the subcutaneous models and at laparotomy in the orthotopic models on day 36. Tumors were imaged with an OV100 Small Animal Imaging System (Olympus, Tokyo, Japan) or a Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon, Tokyo, Japan) and weighed and harvested for analysis.
Hiroshima Y., Zhang Y., Murakami T., Maawy A., Miwa S., Yamamoto M., Yano S., Sato S., Momiyama M., Mori R., Matsuyama R., Chishima T., Tanaka K., Ichikawa Y., Bouvet M., Endo I., Zhao M, & Hoffman R.M. (2014). Efficacy of tumor-targeting Salmonella typhimurium A1-R in combination with anti-angiogenesis therapy on a pancreatic cancer patient-derived orthotopic xenograft (PDOX) and cell line mouse models. Oncotarget, 5(23), 12346-12357.
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6

In vivo Imaging of Tumor-Targeting Bacteria

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The OV100 Small Animal Imaging System, containing an MT-20 light source (Olympus Biosystems, Planegg, Germany) and DP70 CCD camera (Olympus) were used for imaging GFP-labeled S. typhimurium A1-R and orthotopic tumors in live mice [38 (link)].
Hiroshima Y., Zhang Y., Murakami T., Maawy A., Miwa S., Yamamoto M., Yano S., Sato S., Momiyama M., Mori R., Matsuyama R., Chishima T., Tanaka K., Ichikawa Y., Bouvet M., Endo I., Zhao M, & Hoffman R.M. (2014). Efficacy of tumor-targeting Salmonella typhimurium A1-R in combination with anti-angiogenesis therapy on a pancreatic cancer patient-derived orthotopic xenograft (PDOX) and cell line mouse models. Oncotarget, 5(23), 12346-12357.
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7

Multimodal Small Animal Imaging

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The OV100 Small Animal Imaging System (Olympus) containing an MT-20 light source (Olympus) and DP70 CCD camera (Olympus) [38 (link)], IV100 laser scanning microscope (Olympus) [39 (link)], FV1000 laser scanning confocal microscope (Olympus) [40 (link)] and the Maestro fluorescence imaging system (CRi, Caliper, Perkin-Elmer Inc., Hopkinton, MA) were used.
Zhang Y., Miwa S., Zhang N., Hoffman R.M, & Zhao M. (2014). Tumor-targeting Salmonella typhimurium A1-R arrests growth of breast-cancer brain metastasis. Oncotarget, 6(5), 2615-2622.
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8

Intravital Imaging of Angiogenesis in Mice

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The ND-GFP transgenic nude mice were anesthetized with ketamine mixture via s.c. injection. An arc-shaped incision was made in the abdominal skin from the axillary to the inguinal region. The subcutaneous connective tissue was separated to free the skin flap without injuring the vessel. Mice were laid flat and the skin flap was spread and fixed on the flat stand. Gelfoam® was directly imaged with OV 100 Small Animal Imaging system (Olympus Corp., Tokyo, Japan) [Yamauchi et al., 2006 (link)] and FV 1000 confocal microscope (Olympus) [Uchugonova et al., 2011 (link)]. The skin was closed with a 6-0 nylon suture after observation.
Uehara F., Tome Y., Miwa S., Hiroshima Y., Yano S., Yamamoto M., Mii S., Maehara H., Bouvet M., Kanaya F, & Hoffman R.M. (2014). Osteosarcoma cells enhance angiogenesis visualized by color-coded imaging in the in vivo Gelfoam® assay. Journal of cellular biochemistry, 115(9), 1490-1494.
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9

Tracking S. typhimurium A1-R in Cervical Cancer

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To demonstrate S. typhimurium A1-R tumor targeting, the cervical cancer PDOX tumors treated with S. typhimu rium A1-R were resected on day 22 for bacteria culture. The tumor specimens were homogenized and suspended in 1 ml PBS. The suspension was diluted 10 times each up to 1:10,000, then cultured in LB agar medium for 12 h. S. typhimurium A1-R colonies expressing GFP were observed with the OV100 Small Animal Imaging System (Olympus, Tokyo, Japan) [30 , 31 (link)].
Miyake K., Murata T., Murakami T., Zhao M., Kiyuna T., Kawaguchi K., Igarashi K., Miyake M., Lwin T.M., Hozumi C., Komatsu S., Kikuchi T., Bouvet M., Shimoya K., Singh S.R., Endo I, & Hoffman R.M. (2019). Tumor-targeting Salmonella typhimurium A1-R overcomes nab-paclitaxel resistance in a cervical cancer PDOX mouse model. Archives of gynecology and obstetrics, 299(6), 1683-1690.
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10

Nude Mice Xenograft Tumor Model

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Eight-week-old female nude (nu/nu) mice (UCSD breeding colony) were housed in pathogen-free conditions. Tumor cells were washed in PBS, injected (2 × 106 cells in 100 μl of PBS) subcutaneously into right and left flanks of nude mice, and tumor volume (length × width2/2) determined by Vernier caliper measurements over 24 d. Orthotopic tumor growth was initiated by surgical implantation (0.4 × 106 cells in 7 μl of growth factor-depleted Matrigel) within the bursal region surrounding one ovary as described (12 (link)). Primary tumor weight was determined following euthanasia upon dissection. Fluorescent images of the intra-abdominal cavity and internal organs were acquired using an OV100 Small Animal Imaging System (Olympus). Blood was collected by heart puncture following euthanasia, samples were centrifuged, and serum was stored at −80°C. The UCSD Institutional Animal Care and Use Committee approved all mouse procedures.
Tancioni I., Uryu S., Sulzmaier F.J., Shah N.R., Lawson C., Miller N.L., Jean C., Chen X.L., Ward K.K, & Schlaepfer D.D. (2014). FAK inhibition disrupts a β5 integrin signaling axis controlling anchorage-independent ovarian carcinoma growth. Molecular cancer therapeutics, 13(8), 2050-2061.
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