μquant plate reader

Manufactured by Agilent Technologies

Sourced in United States

The μQuant plate reader is a compact and versatile spectrophotometer designed for a wide range of absorbance-based assays. It features a high-performance optical system and a temperature-controlled incubation chamber to ensure accurate and reliable measurements.

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Lab products found in correlation

Brdu cell proliferation kit, by Merck Group (2 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Tempol, by Merck Group (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (1 mentions) Mts reagent, by Promega (1 mentions) Sulforhodamine b srb assay, by Merck Group (1 mentions)

Spelling variants of this product

μQuant (184 citations) μQuant™ reader (7 citations) μQuant microplate plate reader (2 citations)

18 protocols using μquant plate reader

Colorimetric Aptamer-Based Assay for Pesticide Detection

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The AuNPs were purchased from
Nano Composix. Manufacturer specification sheet indicated an average
particle diameter of 9.8 ± 0.8 nm at 9.5 nM particle concentration.
The assay was performed as previously described. In brief, 6 μL
of R12.45 Trunc. at 10 μM in 10% methanol water and 135 μL
of AuNPs from the stock were incubated for 30 min at room temperature.
Targets (atrazine and cyanazine) at varying concentrations (100 nM
to 100 μM, 10% methanol water) in 243 μL total volume
were added to the aptamer–gold mixture and incubated for 30
min at room temperature. Lastly, 6 μL of 1 M sodium chloride
solution was added to each mixture for 5 min. The aggregation pattern
was observed by naked eye, and in addition transferred to a clear
96-well plate for digital imaging, and absorbance scan at 520 and
650 nm with a μQuant plate reader (Biotek). Samples were prepared
in duplicate. Three successful independent assays were performed.
Representative data was averaged and standard deviation was calculated.
Statistical significant difference of the means (p < 0.05) was determined with the one-tailed student t-test.

Abraham K.M., Roueinfar M., Ponce A.T., Lussier M.E., Benson D.B, & Hong K.L. (2018). In Vitro Selection and Characterization of a Single-Stranded DNA Aptamer Against the Herbicide Atrazine. ACS Omega, 3(10), 13576-13583.

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Spectrophotometric Assay of NQO1 Activity

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The NQO1 activity was measured using a spectrophotometric assay as described previously [21 ,30 (link)] with slight modifications. For tissue preparation, rat colonic mucosa and liver were homogenized in buffer (0.05 M Tris-HCl, 1.15% KCl, 1 mM EDTA, pH 7.4) before centrifugation at 12,000× g for 20 min at 4 °C. Supernatants were centrifuged at 100,000× g for 1 h at 4 °C to separate microsomal and cytosolic fractions. The cytosolic fractions were snap-frozen in liquid nitrogen and stored at −80 °C. The cytosolic fraction was mixed (1:4 v/v) with the reaction mixture (25 mM Tris-HCl buffer, 0.67 mg/mL BSA, 0.01% Tween-20 (v/v), 0.03 mM NADP⁺, 1 mM glucose-6-phosphate, 5 μM FAD, 2 unit/mL glucose-6-phosphate dehydrogenase, and 0.72 mM 3-(4,-5-dimethylthiazo-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 50 μM menadione (added immediately before reaction)). The product was measured every 50 s over 5 min at 610 nm in a μQuant plate reader (BioTek, Winooski, VT, USA). The reaction was quenched by the addition of 50 μL of 0.3 mM dicumarol in 25 mM Tris buffer (pH 7.4). The absorbance was measured continuously for another 5 min to correct for non-NQO1 specific activity (background). The protein concentrations were determined using the BioRad assay [31 (link)] with BSA as the standard. The enzyme specific activity was reported as nmol MTT reduced/min/mg protein.

Liu X., Wang Y., Hoeflinger J.L., Neme B.P., Jeffery E.H, & Miller M.J. (2017). Dietary Broccoli Alters Rat Cecal Microbiota to Improve Glucoraphanin Hydrolysis to Bioactive Isothiocyanates. Nutrients, 9(3), 262.

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Cell Viability Assay under Hypoxia

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Cells were treated with DMSO (vehicle control), Tempol (4 mM; Sigma-Aldrich), TMZ (250 μM; Sigma-Aldrich) and the combination of Tempol and TMZ after in vitro hypoxic stress or cell sorting. After incubation at 37 °C for 48 h, the medium was removed from each well, 15 μL 3-94,5-dimethyl-2-yl-2,5-diphenyl-tetrazolium (MTT) (Sigma-Aldrich) solution (2 mg/mL) were added and the plates were incubated at 37 °C for 4 h. The reaction was stopped by the addition of 100 μL of isopropanol/HCl, and the absorbance at 570 nm was recorded on a μQuant plate reader (Bio-Tek).

Chen W.L., Wang C.C., Lin Y.J., Wu C.P, & Hsieh C.H. (2015). Cycling hypoxia induces chemoresistance through the activation of reactive oxygen species-mediated B-cell lymphoma extra-long pathway in glioblastoma multiforme. Journal of Translational Medicine, 13, 389.

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Cell Viability Assay and Synergistic Analysis of Herbal Extracts

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Cell viability was assessed by using a 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Briefly, PC12 cells were seeded onto 96-well plates and incubated at 37 °C under 5 % CO₂ for 24 h. The cells were treated with GE, UR or GUW extracts at concentrations in the range of 0–4000 μg/mL, and the resulting mixtures were incubated for 24 h. The cells were then treated with 0.5 mg/ml MTT and incubated at 37 °C under 5 % CO₂ for 4 h. The medium was carefully aspirated and the remaining formazan crystals in each well were dissolved in dimethylsulfoxide (10 mL). The absorbance characteristics of the formazan solutions in each well were read at 490 nm using a μQuant plate reader (BioTek, Winooski, VT, USA). The relationship between the extract concentration and cell viability was visually determined. The pharmaceutical relationship between the GE and UR in GUW was calculated using a modified version of Burgi’s formula [26 (link)], as follow:

q = \frac{E_{G E + U R}}{E_{G E} + E_{U R} - E_{G E} \times E_{U R}}

E_x: effectiveness of herbal extract x. For this formula, q < 0.85 indicates that the two drugs in question are acting in an antagonistic manner, whereas q > 1.15 indicates that the two drugs are acting in a synergistic manner. Values in between these limits (i.e., 0.85 < q < 1.15) indicate that the effects of the two herbs are additive.

Xian J.W., Choi A.Y., Lau C.B., Leung W.N., Ng C.F, & Chan C.W. (2016). Gastrodia and Uncaria (tianma gouteng) water extract exerts antioxidative and antiapoptotic effects against cerebral ischemia in vitro and in vivo. Chinese Medicine, 11, 27.

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UVB-Induced Cytotoxicity Evaluation

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Sub-confluent NHEKs in KGM in 48-well plates (2 × 10⁴ cells/0.2 mL) were washed with Hank’s buffer, UVB-irradiated (50 mJ/cm²), and test compound treated in KGM for 24 h. Then, cell viability (i.e., percent of living cells) was evaluated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay [27 (link)]. Absorbance of cells exposed to MTT at 2 h was measured at λ 570 nm with a μQuant plate reader (BioTek Instruments, Inc., Winooski, VT, USA). Simultaneously, absorbance at λ 650 nm was measured as turbidity. Differences between measurements were the amount of produced blue formazan.

Ikeoka S., Nakahara T., Iwahashi H, & Mizushina Y. (2016). The Establishment of an Assay to Measure DNA Polymerase-Catalyzed Repair of UVB-Induced DNA Damage in Skin Cells and Screening of DNA Polymerase Enhancers from Medicinal Plants. International Journal of Molecular Sciences, 17(5), 667.

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Fluorescence Measurement of S2973 Strains

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Fluorescence from S2973 strains was measured when the culture optical density at 730 nm was between 0.2 – 0.6. For these experiments, cells were cultured at 38 °C in air in 250 ml glass shaker flasks under 50 μE m⁻² s⁻¹ fluorescent lights. O.D.s and fluorescence were measured for 150 μl of culture in 96-well plastic trays using a Bio-Tek μQuant Plate Reader. Fluorescence measurements were made using an excitation wavelength of 514 nm and emission was monitored at 527 nm.

Knoot C.J., Khatri Y., Hohlman R.M., Sherman D.H, & Pakrasi H.B. (2019). Engineered production of hapalindole alkaloids in the cyanobacterium Synechococcus sp. UTEX 2973. ACS synthetic biology, 8(8), 1941-1951.

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Quantifying Cell Proliferation with BrdU

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The BrdU incorporation was measured using the BrdU Cell Proliferation Kit (Merck, Germany). The cells were seeded in a 96 well plate at a concentration of 0.8x10⁵ cells/ml and left overnight. The next day, the cells were treated with three different concentrations of flavokawain B. 24 h before fixing the cells, 20 μl of BrdU was added to each well. After the respective incubation hours, the cells were fixed and denatured using a fixing solution. The plates were then stored at 4 °C. Then, the plates were washed twice before adding 100 μl of the detector antibody into each well for 1 h. Next, 100 μl of Goat anti-mouse Ig G-HRP conjugated was added for 30 min. Afterwards, the plates were incubated with 100 μl of the TMB substrate for roughly 30 min. Finally, 100 μl of stop solution was added and the absorbance was measured at 450 nm, using the μquant plate reader (Bio-tek Instruments, USA).

Abu N., Akhtar M.N., Yeap S.K., Lim K.L., Ho W.Y., Abdullah M.P., Ho C.L., Omar A.R., Ismail J, & Alitheen N.B. (2016). Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases In vitro. BMC Complementary and Alternative Medicine, 16, 86.

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Cell Viability Assay with ONC201

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Cells were seeded in 96-well plates at a density of 5 × 10⁴ cells/well in 200 μL of complete medium, and incubated with (1.25, 2.5, 5.0 and 10 μM) or without ONC201 treatment for 48, 72, or 96 hrs, respectively. The cell viability was then determined using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) as previously described [33 (link)]. Absorbance was measured at 490 nm using the μQuant plate reader (Biotek, Winooski, VT). Experiments were performed in triplicate.

Ni X., Zhang X., Hu C.H., Langridge T., Tarapore R.S., Allen J.E., Oster W, & Duvic M. (2017). ONC201 selectively induces apoptosis in cutaneous T-cell lymphoma cells via activating pro-apoptotic integrated stress response and inactivating JAK/STAT and NF-κB pathways. Oncotarget, 8(37), 61761-61776.

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Cell Viability Assay of Carvedilol

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JB6 P+ cells were seeded in 96-well plates at 500 cells/well and allowed to attach overnight. 24 hr after seeding, cells were treated with 0.1% DMSO or carvedilol and incubated for 1, 1.5, and 2 weeks at 37°C in 5% CO₂/95% air. Cell viability was determined by the addition of MTS reagent according to the manufacturer’s protocol (Promega, Madison, WI) and absorbance was measured using a Bio-Tek μQuant plate reader with KCJunior software.

Cleveland K.H., Liang S., Chang A., Huang K.M., Chen S., Guo L., Huang Y, & Andresen B.T. (2019). Carvedilol inhibits EGF-mediated JB6 P+ colony formation through a mechanism independent of adrenoceptors. PLoS ONE, 14(5), e0217038.

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BrdU Proliferation Assay of 4T1 Cells

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The BrdU proliferation assay was measured using a BrdU cell proliferation kit (Merck, Germany). Firstly, the 4T1 cells (8 × 10⁴ cells/mL) were seeded in the plates and incubated overnight. The next day, the cells were treated with two different concentrations (IC₅₀=0.27 mg/mL; IC₇₅=0.35 mg/mL) of coconut juice vinegar, added with 20 μL of BrdU and incubated for another 72 h. Later, the cells were fixed and denatured using a fixing solution and stored at 4°C. The plates were washed and then incubated with 100 μL of the detector antibody per well for 1 h. Next, 100 μL of goat anti-mouse immunoglobulin G-horseradish peroxidase (HRP) conjugate was added for 30 min, followed by further incubation with 100 μL of the 3,3′,5,5′-tetramethylbenzidine substrate for 30 min. Finally, 100 μL of stop solution was added to the plates, and the absorbance was measured at 450 nm, using a μQuant plate reader (Bio-Tek Instruments, Vermont, USA).

Mohamad N.E., Yeap S.K., Abu N., Lim K.L., Zamberi N.R., Nordin N., Sharifuddin S.A., Long K, & Alitheen N.B. (2019). In vitro and in vivo antitumour effects of coconut water vinegar on 4T1 breast cancer cells. Food & Nutrition Research, 63, 10.29219/fnr.v63.1616.

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