Ultrashield avance 2 600 mhz

The biopolymer γ-PGA was produced and isolated as previously described [36 (link)]. The obtained polymer was identified by Fourier Transforming Infrared Spectroscopy (FTIR) (Genesis II, Mattson, Geneseo, NY, USA) and Nuclear Magnetic Resonance (¹H-NMR, Bruker Ultrashield AVANCE II 600 MHz, Rheinstetten, Germany). ¹H-NMR Spectra were obtained with 64 scans, an 11 ms pulse width, and a 2.65 s acquisition time. The data was collected and analyzed using Bruker TOPSPIN 2.0 software, Deuterium oxide (D₂O) was used as a solvent. The number average molecular mass (M_n) was determined by conventional aqueous based gel permeation chromatography (GPC) at Smithers Rapra in Shrewsbury, UK. An MZ Hema guard plus 2× Hema Linear column (Cognis Performance Chemicals Ltd., Southampton, UK) was used for analysis. The GPC experiments were carried out in 0.2 M NaNO₃, 0.01 M NaH₂PO₄, at pH 7, with a flow rate of 1 mL/min at 30 °C. The data was collected and analyzed using Polymer Laboratories “Cirrus 3.0” software.

¹H NMR spectra were run in CDCl₃ with tetramethylsilane as the internal standard using a Bruker Ultrashield AVANCE II 600 MHz spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany).