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Rat igg1 κ

Manufactured by Thermo Fisher Scientific
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Rat-IgG1 κ is a type of immunoglobulin G (IgG) antibody that is produced in rats. It has a kappa light chain. The core function of this product is to serve as a research tool for antibody-based applications.

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Lab products found in correlation

Rat anti ly6g, by BioLegend (1 mentions) Anti mouse cd16 cd32, by Thermo Fisher Scientific (1 mentions) Fxcycle violet, by Thermo Fisher Scientific (1 mentions) Rat anti cd11b, by Thermo Fisher Scientific (1 mentions) Rat anti cd31, by BioLegend (1 mentions) Rat anti f4 80, by BioLegend (1 mentions) Facscanto 2, by BD (1 mentions) Il 33, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rat IgG1 κ isotype control FITC Rat IgG1 κ isotype control (eBRG1) PE rat IgG1 κ isotype control Rat IgG1

3 protocols using rat igg1 κ

1

Multiparametric Immunophenotyping of Myeloid Cells

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To reduce nonspecific binding to cells bearing Fc receptors, preincubation of cells with anti-mouse CD16/CD32 (eBioscience) was performed prior to all antibody stainings. Dead cells were excluded by FxCycle Violet (Thermo Fisher Scientific) staining. Stained cells were analyzed using a BD FACSCanto II or analyzed and FACS-sorted using a BD Aria cell sorting platform. The frequencies of individual cell populations were quantified with FlowJo software. FACS antibodies used in this study were: rat anti-CD11b (eBioscience, PE-Cy7), rat anti-CD31 (BioLegend, FITC), rat anti-F4/80 (BioLegend, PE), rat anti-Ly6C (BioLegend, APC-Cy7), rat anti-Ly6G (BioLegend, Pacific Blue), rat anti-Tie2 (Invitrogen, APC) and rat anti-CD206 (BD Biosciences, Alexa Fluor 488). Single-stained controls and an unstained control were used for compensation, rat-IgG1 κ (eBioscience, APC) was used as isotype control.
Jakab M., Rostalski T., Lee K.H., Mogler C, & Augustin H.G. (2022). Tie2 Receptor in Tumor-Infiltrating Macrophages Is Dispensable for Tumor Angiogenesis and Tumor Relapse after Chemotherapy. Cancer Research, 82(7), 1353-1364.
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2

IL-33-induced Eosinophilia Analysis

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Mice were given IL-33 (eBioscience, San Diego, CA) daily by intraperitoneal injection at 0.4 µg per day for a total of 7 injections. For some experiments, mice were also given either anti–IL-5 (TRFK5; eBioscience) or isotype control (rat IgG1 κ; eBioscience) at a dose of 25 µg per mouse by intraperitoneal injection on days -1, 2, and 5. One day after the last injection, cells and serum were collected from blood for eosin staining, flow cytometry, and cytokine protein analysis. Additionally, bone marrow was collected for RNA, cytological, and flow cytometric analysis.
Johnston L.K., Hsu C.L., Krier-Burris R.A., Chhiba K.D., Chien K.B., McKenzie A., Berdnikovs S, & Bryce P.J. (2016). IL-33 precedes IL-5 in regulating eosinophil commitment and is required for eosinophil homeostasis. Journal of immunology (Baltimore, Md. : 1950), 197(9), 3445-3453.
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3

Flow Cytometry Analysis of IL-1β in Mice

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WT and Il1r−/− mice on the C57BL/6J background were from the Jackson Laboratory. All protocols were approved by the NHLBI and NEI Animal Care and Use Committees and followed NIH guidelines for using animals in intramural research. Anti-pro-IL-1β-APC and the corresponding isotype control (rat IgG1κ) were from eBioscience; other antibodies for flow cytometric analysis were from Biolegend.
Wan C.K., He C., Sun L., Egwuagu C.E, & Leonard W.J. (2015). Interleukin-1 Receptor Signaling is Critical for the Development of Autoimmune Uveitis. Journal of immunology (Baltimore, Md. : 1950), 196(2), 543-546.
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