Log In Sign up
The largest database of trusted experimental protocols

Irdye 800cw streptavidin

Manufactured by Rockland Immunochemicals
Visit Supplier

IRDye 800CW Streptavidin is a fluorescent labeling reagent used in various bioanalytical applications. It consists of the near-infrared fluorescent dye IRDye 800CW conjugated to the protein streptavidin, which has a high affinity for the biomolecule biotin. This product can be used to detect and visualize biotinylated targets in techniques such as Western blotting, in-cell imaging, and other bioassays.

Automatically generated - may contain errors

Lab products found in correlation

Human cytokine array kit, by R&D Systems (2 mentions) Odyssey imager, by Rockland Immunochemicals (2 mentions) Proteome profiler mouse cytokine array panel a, by R&D Systems (1 mentions)

3 protocols using irdye 800cw streptavidin

1

Cytokine Profiling of Ischemic Mouse Cardiac Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adult mouse cardiac fibroblasts were isolated as described above. Cells were subjected to in vitro ischemia buffer27 and supernatant was harvested for use with the Proteome Profiler Mouse Cytokine Array Panel A (R&D ARY006) with one modification: IRDye 800CW Streptavidin (Rockland S000-31) was used.
Woodall M.C., Woodall B.P., Gao E., Yuan A, & Koch W.J. (2016). Cardiac Fibroblast GRK2 Deletion Enhances Contractility and Remodeling Following Ischemia/Reperfusion Injury. Circulation research, 119(10), 1116-1127.
+ Open protocol
+ Expand
2

Cytokine Profiling using Array

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed once with PBS and incubated for 8 h with fresh media. Supernatant was collected, cleared by centrifugation, and used immediately. The amount of supernatant used was normalized to cell number and used with the human cytokine array kit (R&D Systems). IRDye 800CW Streptavidin (Rockland) was used as secondary (1:2,000) and arrays were imaged and quantified on an Odyssey Imager.
Catanzaro J.M., Sheshadri N., Pan J.A., Sun Y., Shi C., Li J., Powers R.S., Crawford H.C, & Zong W.X. (2014). Oncogenic Ras induces inflammatory cytokine production by up-regulating the squamous cell carcinoma antigens SerpinB3/B4. Nature communications, 5, 3729.
+ Open protocol
+ Expand
3

Cytokine Profiling using Array

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed once with PBS and incubated for 8 h with fresh media. Supernatant was collected, cleared by centrifugation, and used immediately. The amount of supernatant used was normalized to cell number and used with the human cytokine array kit (R&D Systems). IRDye 800CW Streptavidin (Rockland) was used as secondary (1:2,000) and arrays were imaged and quantified on an Odyssey Imager.
Catanzaro J.M., Sheshadri N., Pan J.A., Sun Y., Shi C., Li J., Powers R.S., Crawford H.C, & Zong W.X. (2014). Oncogenic Ras induces inflammatory cytokine production by up-regulating the squamous cell carcinoma antigens SerpinB3/B4. Nature communications, 5, 3729.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!