The Fourier transform infrared spectroscopy analysis (FT-IR) spectra of drug-free NPs, drug-free pD/NPs and drug-free Apt-pD/NPs was recorded by FT-IR spectrophotometer (Thermo Nicolet, Madison, Wisconsin) using KBr.
Ftir spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FTIR spectrophotometer is an analytical instrument used to identify and analyze the chemical composition of materials. It operates by measuring the absorption of infrared radiation by a sample, which produces a unique spectrum that can be used to identify the presence and concentration of various functional groups and molecular structures within the sample.
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Lab products found in correlation
Escalab 250xi, by Thermo Fisher Scientific (4 mentions)
Nicolet 6700, by Thermo Fisher Scientific (3 mentions)
Tecnai g2 20, by Thermo Fisher Scientific (2 mentions)
Jen 1230, by JEOL (2 mentions)
Sta 449, by Netzsch (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Tetramethylethylenediamine, by Merck Group (1 mentions)
Bromophenol blue, by Merck Group (1 mentions)
Coomassie brillant blue cbb g250, by Merck Group (1 mentions)
Cbb r250, by Merck Group (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Electrophoresis system, by Bio-Rad (1 mentions)
Uv vis spectrophotometer, by Thermo Fisher Scientific (1 mentions)
8000 series, by Shimadzu (1 mentions)
F 4500 fluorescence spectrophotometer, by Hitachi (1 mentions)
Graphite rod, by Merck Group (1 mentions)
Sucrose, by Thermo Fisher Scientific (1 mentions)
40 protocols using ftir spectrophotometer
1
FT-IR Spectroscopy of Drug-free Nanoparticles
2
Structural Analysis of Sialic Acid-Chitosan Complex
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The structure analysis of the sialic acid and chitosan complex was examined by Fourier transform infrared spectroscopy (FT-IR). The IR spectra of the samples were recorded by an FT-IR spectrophotometer (Thermo Nicolet, Madison, WI, USA). The spectrum was scanned in transmission mode from 400 to 4000 cm−1 wavenumber range. The dry samples were blended with KBr powder at a ratio of 100:1 and pressed into a disk before spectrum acquisition.
3
FT-IR Analysis of Drug-Free Nanoparticles
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The Fourier transform infrared (FT-IR) spectroscopy analysis of drug-free NPs, drug-free pD-NPs, and drug-free Apt-pD-NPs was recorded by FT-IR spectrophotometer (Thermo Nicolet, Madison, WI, USA) using KBr.
4
FT-IR Analysis of Purified EPS from L. plantarum
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The infrared analysis of purified EPS from the two L. plantarum strains was carried out using an FT-IR spectrophotometer (Thermo Nicolet, USA) in the spectrum ranges of 400–4,000 cm−1 for the detection of functional groups present in the samples.
5
Characterization of Functionalized Nanoparticles
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The particle size and surface zeta potential of the prepared nanoparticles were measured by a Zetasizer Nano ZS (Malvern Instruments, Malvern, UK). The shape and surface morphology of the synthesized NPs were observed by transmission electron microscopy (TEM; FEI Company, Hillsboro, OR, USA). The Fourier transform infrared (FT-IR) spectra of MSNs-anti-miR-155@PDA-Apt was recorded by FT-IR spectrophotometer (Thermo Nicolet, Madison, WI, USA). Thermogravimetric analysis (TGA) of the NPs was performed by Netzsch STA 449 (Netzsch, Selb, Germany).
Finally, the conjugation of AS1411 APt to MSNs@PDA was further confirmed by agarose gel electrophoresis. Samples (MSNs@PDA, MSNs@PDA-Apt, AS1411 aptamer, and DNA ladder) were loaded into a 1% agarose gel. Electrophoresis was carried out at 80 V for 1.5 hours in Tris-Borate EDTA solution. The gel was then stained with ethidium bromide and imaged using a MultiImage™ Light Cabinet (Alpha Innotech Corporation, San Leandro, CA, USA).
Finally, the conjugation of AS1411 APt to MSNs@PDA was further confirmed by agarose gel electrophoresis. Samples (MSNs@PDA, MSNs@PDA-Apt, AS1411 aptamer, and DNA ladder) were loaded into a 1% agarose gel. Electrophoresis was carried out at 80 V for 1.5 hours in Tris-Borate EDTA solution. The gel was then stained with ethidium bromide and imaged using a MultiImage™ Light Cabinet (Alpha Innotech Corporation, San Leandro, CA, USA).
6
Extraction and Characterization of Antioxidants from Damson Plums
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Material HCI, NaOH, Sepharose 4B and 6B, CNBr, NaHCO 3 , L-Tyrosine, p-aminobenzoic acid, NaNO 2 , Na 2 HPO 4 *2H 2 O, K 2 HPO 4 , polyethylene glycol, ascorbic acid, (NH 4 ) 2 SO 4 , NaCI, catechol, 4-methyl catechol, pyrogallol, caffeic acid, bovine serum albumin (BSA), Tris base, acrylamide/bis 30%, sodium dodecyl sulfate, Tetramethyl ethylene diamine, ammonium persulfate, glycerol, b-mercapto ethanol, bromophenol blue, coomassie brillant blue (CBB) G250, CBB R250, acetic acid, methanol were purchased from Sigma-Aldrich Co and Merck. Multicolor broad range and prestained protein marker (10 to 260 kDa) were purchased from thermos scienti c. Electrophoresis system was supplied from Bio-Rad. Dialysis membrane (D-9777) and 18mL liquid chromatography column as 1.5 cm*10 cm were purchased from Sigma-Aldrich Co. Damson plums (Prunus insititia) was supplied from Kocaeli. Spectrophotometric UV analyses were performed in Shimadzu UV-VIS Spectrophotometer and IR analyses in Thermo-Scienti c FT-IR spectrophotometer.
7
Characterizing Aspergillus neoalliaceus Morphology
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The surface morphology of I heat-inactivated Aspergillus neoalliaceus was monitored with GAIA3 + Oxford XMax 150 EDS scanning electron microscope. Fungal samples characterized spectroscopically by 8000 Series (Shimadzu, Japan) FTIR spectrophotometer (Thermo Scienti c).
Fourier transform infrared spectrums of lead-loaded and unloaded fungal masses were recorded between 4000 and 400 cm - 1 wavenumber regions. The net surface charge of heat-inactivated fungal biomass was analyzed by measuring zeta potentials at known pH values within 3.0 to 7.0.
Fourier transform infrared spectrums of lead-loaded and unloaded fungal masses were recorded between 4000 and 400 cm - 1 wavenumber regions. The net surface charge of heat-inactivated fungal biomass was analyzed by measuring zeta potentials at known pH values within 3.0 to 7.0.
8
FTIR Analysis of Collagen Structure
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FTIR spectrophotometer (Thermo Scientific, USA) was used to record the FTIR spectrum of the isolated PSCs to obtain further information on the collagen secondary structure with regards to different isolation methods. The spectrum was recorded from 500 to 4000 cm−1, with a resolution of 4 cm−1 and average scans of 64 [9 (link)].
9
FTIR Analysis of Quince Leaves and Ag NPs
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The silver nanoparticles and quince leaves were examined using FTIR spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA), in the wavenumber range between 4000 and 400 cm−1 and 2 cm−1 resolution. The dry powders of quince leaves and Ag NPs were used for FTIR study.
10
FTIR Analysis of Fluconazole-Chitosan Complexes
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FTIR spectra of pure fluconazole, chitosan, anionic polymers, physical mixture and PECs were recorded using signal/noise ratio (S/N) of 30,000:1. Potassium bromide (KBr) disc technique via FTIR spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) was used for scanning spectral region of 4000 cm−1 to 400 cm−1 [15 (link)].
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