Cells were seeded into 96‐well plates at a density of 3 × 103 (SUIT‐2) and 8 × 103 (AsPC‐1) cells/well, then transfected with siRNAs 24 h later. Culture medium was replaced with that containing 0.5% FBS 24 h after transfection, and cells incubated for indicated times. Cell viability was evaluated using a WST‐1 assay (Premix WST‐1 Cell Proliferation Assay; Takara Bio). In an additional analysis using a GJB4 cDNA ORF clone and MET inhibitor (capmatinib; MedChemExpress), the concentration of capmatinib used was set at 500 nM based on previous reports and our analyses.
18 (link) SUIT‐2 cells were seeded into 6‐well plates at a density of 3 × 106 cells/well; after 12 h, cells were then transfected with cDNA ORF. Culture medium was replaced with that containing 0.5% FBS 12 h after transfection, and cells seeded into 96‐well plates at a density of 3 × 103 cells/well. Cells were incubated for a further 24 h, and exposed to capmatinib or DMSO.
18 (link) SUIT‐2 cells were seeded into 6‐well plates at a density of 3 × 106 cells/well; after 12 h, cells were then transfected with cDNA ORF. Culture medium was replaced with that containing 0.5% FBS 12 h after transfection, and cells seeded into 96‐well plates at a density of 3 × 103 cells/well. Cells were incubated for a further 24 h, and exposed to capmatinib or DMSO.