HIF-1α and ARNT were produced by IVTT using the TNT T7 Coupled Coupled Reticulocyte Lysate System (Promega). Nucleotide probes were designed based off of the previously characterized CA9 HIF1 binding site (Wykoff et al., 2000 (link)): 5′-CCAATGCAC GTACAGCCC-3′ and 5′-GGGCTGTAC GTGCATTGG-3′ with the underlined cytosine being unmodified, methylated, or hydroxymethylated.
Tnt t7 coupled coupled reticulocyte lysate system
Manufactured by Promega
The TNT T7 Coupled Reticulocyte Lysate System is a cell-free in vitro transcription and translation system. It is used to express recombinant proteins from DNA templates containing a T7 RNA polymerase promoter.
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Lab products found in correlation
2 protocols using tnt t7 coupled coupled reticulocyte lysate system
1
HIF-1α Binding Site Nucleotide Probes
2
HIF-1α Binding Site Nucleotide Probes
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