Dsu spinning disc confocal microscope

Manufactured by Olympus

Sourced in Panama

The DSU spinning disc confocal microscope is a high-speed imaging system designed for live-cell microscopy. It features a spinning disc that rapidly scans the sample, allowing for the acquisition of high-quality fluorescence images with minimal phototoxicity to the specimen.

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Lab products found in correlation

Imagem enhanced c9100 13, by Hamamatsu Photonics (1 mentions) Tcs sp5 2 confocal microscope, by Leica (1 mentions) Application suite, by Leica (1 mentions) Ix 81 dsu spinning disc confocal microscope, by Olympus (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Cell m imaging software, by Olympus (1 mentions) Mouse map2, by Merck Group (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Ix81 microscope, by Olympus (1 mentions) Ix81 epifluorescence microscope, by Olympus (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Developmental Studies Hybridoma Bank (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions)

Close spelling variants of this product

IX 81 DSU Spinning Disc Confocal microscope Spinning Disc confocal microscope DSU-IX81 Olympus IX2-UCB DSU spinning disc confocal microscope DSU Spinning Disc Confocal Olympus DSU Spinning Disc microscope Spinning-disc confocal microscope DSU confocal microscope DSU microscope Confocal microscope

5 protocols using dsu spinning disc confocal microscope

Confocal Microscopy Imaging of Midgut

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Images were obtained using a either a DSU spinning disc confocal
microscope (Olympus) equipped with UPLFLN ×20, ×40 oil
immersion and ×60 oil-immersion objectives and 512×512
EM-CCD camera (ImagEM Enhanced C9100-13, Hamamatsu Photonics) or a Leica
TCS SP5 II confocal microscope (Leica Microsystems) equipped with an
×40 oil-immersion and ×63 oil-immersion objective, 405
nm diode, 458, 476, 488, 496 and 514 nm Ar, 543 nm HeNe and 633 nm HeNe
lasers and digital zoom. Images were acquired using SlideBook (version
4.2, Intelligent Imaging Innovations) or Leica Application Suite (Leica
Microsystems) software.
For all images, Z-stacks through the midgut were acquired every
0.2–1 μm through the tissue.

Lucchetta E.M, & Ohlstein B. (2017). Amitosis of polyploid cells regenerates functional stem cells in the Drosophila intestine. Cell stem cell, 20(5), 609-620.e6.

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Immunofluorescence Analysis of Neuronal Markers

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Immunofluorescence analysis was performed as previously described (Posada-Duque et al. 2013 (link)). Briefly, the cells were incubated overnight at 4°C with the following primary antibodies: mouse PSD95 (1:250, Calbiochem), rabbit PSD95 (1:250, Cell Signaling) and mouse MAP2 (1:2000, Sigma). The Alexa-594 fluorescent antibody was used as a secondary antibody (1:1000, Molecular Probes). The nuclei were stained with Hoechst 33258 (1:5000, Invitrogen), and the cells were incubated with phalloidin conjugated with Alexa 594 (1:2000, Molecular probes) for 1 h. The cells were washed 4 times in buffer, coverslipped using Gel Mount (Biomeda), and observed under an Olympus IX 81 epifluorescence microscope or a DSU Spinning Disc Confocal microscope. No staining was observed when the primary antibodies were omitted.
XY images were collected using an Olympus IX 81 microscope with 10× (NA, 0.4), 40× (NA, 1.3) or 60× (NA, 1.42) oil-immersion objectives. Z-stack images were collected at 0.4-µm intervals on an Olympus IX 81 DSU Spinning Disc Confocal microscope (60× oil-immersion; NA, 1.42). The image stacks were deconvolved using Cell^M imaging software (Olympus).

Posada-Duque R.A., López-Tobón A., Piedrahita D., González-Billault C, & Cardona-Gomez G.P. (2015). p35 and Rac1 underlie the neuroprotection and cognitive improvement induced by CDK5 silencing. Journal of neurochemistry, 134(2), 354-370.

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Immunostaining of Cultured Dorsal Root Ganglia

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After five days in culture, 1 ml of room temperature PBS containing 8% (w/v) paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) was added to the culture solution for a final concentration of 4% paraformaldehyde. After 30 min, the paraformaldehyde was removed and washed three times with PBS before a PBS blocking solution containing 10% fetal bovine serum (FBS, Invitrogen, Life Technologies, Carlsbad, CA) and 0.1% Triton X-100 was added to the cells and incubated at 4°C. After 12 h, the blocking solution was removed and a PBS solution containing 2% of FBS, 0.1% Triton X-100, and RT-97 antibody to label neurofilament (1:100, Developmental Studies Hybridoma Bank, Iowa City, IA) was added to the DRG cultures. After 8 h, the solution was removed, and the DRG were washed three times with PBS. Then, a PBS solution containing 2% of FBS, 0.1% Triton, and donkey anti-mouse antibody conjugated with Alexa-594 (1:1000 dilution, Sigma-Aldrich, St. Louis, MO) was added to the DRG cultures and incubated for 1 h. Finally, a solution of DAPI (1:1000, Sigma-Aldrich, St. Louis, MO) was added for 15 min before washing the cells three times with PBS. The cells were imaged with an Olympus DSU spinning disc confocal microscope (Center Valley, PA).

Schaub N.J., Le Beux C., Miao J., Linhardt R.J., Alauzun J.G., Laurencin D, & Gilbert R.J. (2015). The Effect of Surface Modification of Aligned Poly-L-Lactic Acid Electrospun Fibers on Fiber Degradation and Neurite Extension. PLoS ONE, 10(9), e0136780.

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Quantifying Stress Granules in Yeast

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Standard confocal microscopy was completed as in Wallace et al. [2015], generally using Pab1-Clover as the SG marker unless otherwise noted. Cells were grown to log-phase as previously described. 1mL of cells were transferred to 1.5mL Eppendorf tubes. For heat stress, cells were shocked in a heat block, spun down in a microfuge, and 950 uL of supernatant were removed. For azide stress, 10% (w/v) azide or water was added directly to the 1mL of cells to proper dilution of aizde. For ethanol stress, cells were spun down in microfuge and resuspended in media with appropriate amounts of ethanol. 1.5 uL of treated cells were then placed on a glass slide and imaged immediately. For AID treatment, cells were treated as previously described, and were imaged immediately after a 2 hour exposure to Auxin. For cycloheximide treatment, cells were exposed to 100 ug/mL of cycloheximide for 10 minutes, stressed for 10 minutes, and then imaged immediately. Cells were imaged on an Olympus DSU spinning disc confocal microscope using a 100× 1.45 TIFM oil objective (PlanApo) and the FITC filter cube for the Clover fluorophore in Z-stacks. Representative images are maximum projections of the collected z-stacks. Maximum projection images of the cells were used to quantify the number of stress granules per cell using CellProfiler.

Glauninger H., Bard J.A., Wong Hickernell C.J., Airoldi E.M., Li W., Singer R.H., Paul S., Fei J., Sosnick T.R., Wallace E.W, & Drummond D.A. (2024). Transcriptome-wide mRNA condensation precedes stress granule formation and excludes stress-induced transcripts. bioRxiv.

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Stress Granule Quantification Protocol

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Standard confocal microscopy was completed as in Wallace et al. [2015], generally using Pab1-Clover as the SG marker unless otherwise noted. Cells were grown to log-phase as previously described. 1mL of cells were transferred to 1.5mL Eppendorf tubes. For heat stress, cells were shocked in a heat block, spun down in a microfuge, and 950 uL of supernatant were removed. For azide stress, 10% (w/v) azide or water was added directly to the 1mL of cells to proper dilution of aizde. For ethanol stress, cells were spun down in microfuge and resuspended in media with appropriate amounts of ethanol. 1.5 uL of treated cells were then placed on a glass slide and imaged immediately. For AID treatment, cells were treated as previously described, and were imaged immediately after a 2 hour exposure to Auxin. For cycloheximide treatment, cells were exposed to 100 ug/mL of cycloheximide for 10 minutes, stressed for 10 minutes, and then imaged immediately. Cells were imaged on an Olympus DSU spinning disc confocal microscope using a 100x 1.45 TIFM oil objective (PlanApo) and the FITC filter cube for the Clover fluorophore in Z-stacks. Representative images are maximum projections of the collected z-stacks. Maximum projection images of the cells were used to quantify the number of stress granules per cell using CellProfiler.

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