Poloxamer 407 (Pluronic F127) and Pluronic L 31 were purchased from Sigma-Aldrich-Chemie (Steinheim, Germany). Poloxamer-based binary hydrogels were prepared by the cold method [38 (link)]. To prepare blank poloxamer-based hydrogel (B-PbH), accurately weighed amounts of P127 (20% w/w) and P31 (1% w/w) were added to purified water cooled to 4 °C. The mixture was kept in the refrigerator at least 24 h and periodically stirred until a clear, homogenous solution was obtained, thus ensuring the complete dissolution of the poloxamers. Poloxamer-based binary formulation containing OEO (5% w/w) was prepared by the same method, adding the volatile oil to the cold aqueous solution of the two poloxamers under continuous stirring until a clear, homogenous hydrogel was obtained.
Pluronic l 31
Manufactured by Merck Group
Sourced in Germany
Pluronic L 31 is a nonionic, water-soluble block copolymer surfactant. It is composed of a central hydrophobic block of polyoxypropylene (POP) flanked by two hydrophilic blocks of polyoxyethylene (POE). The product is a clear, viscous liquid at room temperature.
Automatically generated - may contain errors
Lab products found in correlation
Poloxamer 407 pluronic f127, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Pluronic f 127, by Merck Group (1 mentions)
Talos f200x g2, by Thermo Fisher Scientific (1 mentions)
Jem 2010fx, by JEOL (1 mentions)
Pluronic l64, by Merck Group (1 mentions)
Pluronic p123, by Merck Group (1 mentions)
Pluronic f108, by Merck Group (1 mentions)
Jsm 6700f, by JEOL (1 mentions)
Poloxamer 407, by Merck Group (1 mentions)
Brij c10, by Merck Group (1 mentions)
Citric acid monohydrate, by ITW Reagents (1 mentions)
Sodium phosphate dibasic heptahydrate, by ITW Reagents (1 mentions)
7 protocols using pluronic l 31
1
Poloxamer-Based Hydrogel Formulations
2
Poloxamer Binary Hydrogels for Chorioallantoic Membrane
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To prepare the poloxamer binary hydrogels, we used Poloxamer 407 (Pluronic F127, CAS no.: 9003-11-6, product number: P2443) and Pluronic L 31 (product number: 435406), acquired from Sigma-Aldrich-Chemie (Steinheim, Germany). For the chorioallantoic membrane assay, sodium dodecyl sulfate (SDS, CAS no.: 151-21-3; MW 288.38 g/mol) and leptin from mice (L3772, CAS no.: 181030-10-4; predicted MW~16 kDa) were purchased from Sigma-Aldrich-Chemie (Steinheim, Germany). All used reagents were analytical-grade.
3
Characterization of Gold Nanoparticles
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Commercial
chemicals including Pluronic L-31 (Mn ≈
1100), L-64 (Mn ≈ 2900), F-68 (Mn ≈ 8400),
F-127 (Mn ≈ 12 600), and
F-108 (Mn ≈ 14 600) were
bought from Sigma–Aldrich and were used without further purification.
A JAC ultrasonic 1505 (150 W, 40 KHz) was used for sonication. TEM
images were obtained with a Talos F200x G2 (FEI, Thermo Scientific)
with an operating voltage of 200 keV. For measuring optical absorbance
spectra of gold NPs (250–1200 nm), a JASCO V-770 spectrophotometer
was used with a 1 cm cell path length and with a UV/vis bandwidth
of 2.0 nm and an NIR bandwidth of 8.0 nm.
chemicals including Pluronic L-31 (Mn ≈
1100), L-64 (Mn ≈ 2900), F-68 (Mn ≈ 8400),
F-127 (Mn ≈ 12 600), and
F-108 (Mn ≈ 14 600) were
bought from Sigma–Aldrich and were used without further purification.
A JAC ultrasonic 1505 (150 W, 40 KHz) was used for sonication. TEM
images were obtained with a Talos F200x G2 (FEI, Thermo Scientific)
with an operating voltage of 200 keV. For measuring optical absorbance
spectra of gold NPs (250–1200 nm), a JASCO V-770 spectrophotometer
was used with a 1 cm cell path length and with a UV/vis bandwidth
of 2.0 nm and an NIR bandwidth of 8.0 nm.
4
Synthesis and Characterization of Pluronic Copolymer Nanostructures
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All chemicals including Pluronic triblock copolymers (CAS 9003-11-6) with the number average molecular weights (Mns) of ∼1100 (product name Pluronic L-31), ∼2900 (Pluronic L-64), ∼5800 (Pluronic P-123), and ∼14 600 (Pluronic F-108) were purchased from Sigma-Aldrich. Aqueous CTAB solution (0.2 M) was recrystallized twice. X-ray diffraction (XRD) patterns were obtained with Rigaku Ultima IV diffractometer using CuKα radiation (λ = 1.54056 Å) in θ–θ mode. JAC ultrasonic 1505 (150 W, 40 kHz) was used for sonication. Transmission electron microscopy (TEM) images were obtained with JEOL JEM 2010FX operating at 200 kV. Scanning electron microscopy (SEM) images were taken with JEOL JSM 6700F with an operating voltage of 5.0 kV. Fourier transform infrared (FTIR) spectra were recorded with JASCO FTIR-4600 type A, ranging from 650 cm−1 to 4000 cm−1 with a resolution of 4 cm−1 and with an average scan of 8. Without adding KBr or making KBr pellets, sample was placed on the single-reflection ATR accessory and the spectra were measured while the pressure applicator was implemented.
5
Poloxamer-based Hydrogel with Oregano Oil
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To obtain the poloxamer-based binary hydrogel loaded with OEO, oregano essential oil previously extracted from the species O. vulgare var. vulgare and characterized [51 (link)], Poloxamer 407 (trade name Pluronic® F127), Pluronic L 31 purchased from Sigma-Aldrich-Chemie (Steinheim, Germany), and purified water were used.
6
Preparation of Surfactant-Assisted SIO
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SIO was purchased from Khonkaen Kasettagram Hitec Center, Nai Muang, Muang, Khonkaen, Thailand. Nonionic surfactants, polyoxyethylene sorbitan monooleate (Tween ® 80; Mw＝1310) , Brij ® C-10 (Mw＝683) , and Pluronic ® L-31 (Mw＝1100) were purchased from Sigma Aldrich (St. Louis, MO) . Fresh distilled water was prepared in our laboratory and used throughout the experiment.
7
Cloud point determination of triblock copolymers
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All studied triblock copolymers studied in this work were purchased from Sigma-Aldrich, namely Pluronic L-31, L-35, 31R1 and 10R5 (see Table S1). Both the imidazolium: McIlvaine buffer (0.18 M) at pH 7.0 was used as solvent, being composed of sodium phosphate dibasic heptahydrate (purity ≥ 99%) and citric acid monohydrate (purity ≥ 99%), which were obtained from Panreac AppliChem.
The cloud point determination was established in McIlvaine buffer solutions, since this is the medium of preference to work with pH sensitive or labile biomolecules, and at copolymer concentrations ranging from 0.5 to 17.5 wt% and SAIL concentrations of 0.3 wt%. For each system, three replicas were determined, and the average value calculated. The cloud points were measured through the visual determination of the onset turbidity of a solution heated in a temperature controlled water bath with an error of ± 0.01 o C following a methodology described elsewhere. 30, 31 The full results are shown in Figure S1.
The cloud point determination was established in McIlvaine buffer solutions, since this is the medium of preference to work with pH sensitive or labile biomolecules, and at copolymer concentrations ranging from 0.5 to 17.5 wt% and SAIL concentrations of 0.3 wt%. For each system, three replicas were determined, and the average value calculated. The cloud points were measured through the visual determination of the onset turbidity of a solution heated in a temperature controlled water bath with an error of ± 0.01 o C following a methodology described elsewhere. 30, 31 The full results are shown in Figure S1.
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