Bx50 bxfla microscope

The livers and intestines preserved in formalin were processed using the paraffin embedding technique, following the guidelines outlined in Bancroft and Gamble³⁷ . Tissue slices were stained with hematoxylin and eosin, and observed using an Olympus BX50/BXFLA microscope (Japan).

The histopathological study was carried out by following the method of Gewaily et al. [39 (link)], where three fish per aquarium (N = 9) were collected, and their viscera were dissected. Then, the intestines, livers, spleens, and gills were separated and fixed in Bouin’s solution for 18–24 h. The tissues were dehydrated using alcohol, cleared in xylene, and embedded in paraffin wax [43 ]. Then, 5 μm thick sections were obtained with a rotatory microtome (RM 20352035; Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin stain. Finally, the stained tissue sections were viewed and imaged with a digital camera connected to a BX50/BXFLA microscope (Olympus, Tokyo, Japan).

The histological examination was adopted according to Gewaily et al. [45 ]. Five fish were randomly selected from each treatment. After deep anaesthesia, the intestine samples were obtained and cut into pieces of approximately 0.5 cm. The samples were fixed in 10% neutral buffered formaldehyde for 24 h, dehydrated in ascending grades of alcohol, cleared with xylene, and embedded in paraffin wax. Five-μm thick sections were cut using a rotatory microtome (RM 20352035; Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin (H&E), then examined using a BX50/BXFLA microscope (Olympus, Tokyo, Japan).