Mtesr1 plus

Manufactured by STEMCELL

Sourced in United States, Denmark, China

MTeSR1 plus is a serum-free and feeder-free medium optimized for the culture of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). It supports the maintenance of these cells in an undifferentiated state.

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Lab products found in correlation

Versene solution, by Thermo Fisher Scientific (2 mentions) Mtesr1, by STEMCELL (2 mentions) Matrigel, by Merck Group (1 mentions) Matrigel, by Corning (1 mentions) Relesr, by STEMCELL (1 mentions) Hes qualified matrigel, by Corning (1 mentions) Rhlaminin 521, by BioLamina (1 mentions) Bioni010 c, by Bioneer (1 mentions) Revitacell, by Thermo Fisher Scientific (1 mentions) Egm 2 medium, by PromoCell (1 mentions) Huvecs, by Lonza (1 mentions) Flow nanoanalyzer, by NanoFCM (1 mentions) Matrigel coated plate, by Corning (1 mentions) Ssea4, by Santa Cruz Biotechnology (1 mentions) Epi5 episomal ipsc reprogramming kit, by Thermo Fisher Scientific (1 mentions) Leukocyte alkaline phosphatase kit, by Merck Group (1 mentions) Matrigel, by BD (1 mentions)

Close spelling variants of this product

MTeSR1 (551 citations) MTeSR1 Plus medium (11 citations) MTESR1 plus media (2 citations)

8 protocols using mtesr1 plus

Human Induced Pluripotent Stem Cell Culture

Cited 1 time

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All hiPSCs were previously validated with respect to their stemness and differentiation capacity (91 (link)). The hiPSCs were routinely tested for mycoplasma. In total, four hiPSC lines from two donors were included. Approval for this study was obtained from the Stanford Institutional Review Board, and informed consent was obtained from all donors. hiPSCs were maintained in their pluripotent state by being cultured with mTESR-1 Plus (STEMCELL Technologies) media in monolayer on human embroyonic stem cell (hESC)–qualified Matrigel (0.1 mg/ml; Sigma-Aldrich).

Roth J.G., Huang M.S., Navarro R.S., Akram J.T., LeSavage B.L, & Heilshorn S.C. (2023). Tunable hydrogel viscoelasticity modulates human neural maturation. Science Advances, 9(42), eadh8313.

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Maintenance of Reprogrammed iPSCs

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The iPSCs obtained from reprogramming were maintained in culture on 6-well plates coated with Matrigel (Cod. 354277, Corning, New York, NY, USA) in mTeSR1 plus (Cod. 05826, Stem Cell Technologies, Vancouver, Canada and incubated at 37 °C, 5% CO₂. The medium was changed every other day, and when the cells reached 7080–% confluence they were split and transferred to new plates.

Marioli C., Magliocca V., Petrini S., Niceforo A., Borghi R., Petrillo S., La Rosa P., Colasuonno F., Persichini T., Piemonte F., Massey K., Tartaglia M., Moreno S., Bertini E, & Compagnucci C. (2020). Antioxidant Amelioration of Riboflavin Transporter Deficiency in Motoneurons Derived from Patient-Specific Induced Pluripotent Stem Cells. International Journal of Molecular Sciences, 21(19), 7402.

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Monolayer Culturing of Human Pluripotent Stem Cells

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Experiments using hPSCs were approved by the Stem Cell Oversight Committee (SCRO) of University of Southern California under protocol # 2018-2. Human pluripotent stem cells are routinely cultured in mTeSR1 (STEMCELL Technologies #85850) or mTeSR1 Plus (STEMCELL Technologies #100-0276) medium in monolayer culture format coated with Matrigel and passaged using dispase as previously described ¹, or using Versene Solution (Thermo Fisher # 15040066) following manufacturer’s protocols.

Huang B., Zeng Z., Li H., Li Z., Chen X., Guo J., Zhang C.C., Schreiber M.E., Vonk A.C., Xiang T., Patel T., Li Y., Parvez R.K., Der B., Chen J.H., Liu Z., Thornton M.E., Grubbs B.H., Diao Y., Dou Y., Gnedeva K., Lindström N.O., Ying Q., Pastor-Soler N.M., Fei T., Hallows K.R., McMahon A.P, & Li Z. (2023). Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids. bioRxiv.

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Differentiated Sensory Neuron Co-culture

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hiPS cell line was generated from 2-year-old male foreskin fibroblasts ordered from Coriell institute (AG07095). hiPS cell line was maintained with daily media change of mTeSR1 plus (StemCell technologies) and passaging every 4-5 days using ReLeSR (StemCell technologies). hES-qualified Matrigel (Corning) was used as a matrix. Calu3 cells and HEK293T cells were cultured using DMEM, 10% FBS, L-Glutamine, Pen/Strep and non-essential amino acids. All cells were cultured at 37°C with 5% of CO₂.
For the co-cultures, HEK293T cells were resuspended in Neuro maturation media (BrainPhys media, N2 supplement (1X), B27 supplement (1X), BDNF (10ng/ml), GDNF (10ng/ml), NT3 (10ng/ml), Pen/Strep and non-essential amino acids) and added to terminally differentiated sensory neurons. Co-cultures were analyzed or infected after 48h at 37°C.

Flamier A., Bisht P., Richards A., Tomasello D.L, & Jaenisch R. (2023). Human iPS cell-derived sensory neurons can be infected by SARS-CoV-2. iScience, 26(9), 107690.

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Culturing hiPSC and Derived Cell Types

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All work with hiPSC and the derived cell types was performed under the respective Swiss legislation, ethical guidelines and approval. hiPS_SFC_086 (generated by the StemBANCC Innovative Medicines Initiative consortium [27 (link)]; https://www.imi.europa.eu/projects-results/project-factsheets/stembancc, accessed on 20 December 2021) and BIONi010-C (Bioneer, DK-2970 Hørsholm, Denmark) lines were cultured in mTeSR1 Plus (StemCell Technologies, Vancouver, BC, Canada) on 12.5 µg/mL rhLaminin-521 (BioLamina, Sundbyberg, Sweden) coated plates. RevitaCell (Gibco, Waltham, MA, USA) would be supplemented into the medium for splitting and thawing.

Bull D., Schweitzer C., Bichsel C., Britschgi M, & Gutbier S. (2022). Generation of an hiPSC-Derived Co-Culture System to Assess the Effects of Neuroinflammation on Blood–Brain Barrier Integrity. Cells, 11(3), 419.

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Maintenance and Validation of hPSCs and HUVECs

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hPSCs were maintained on Matrigel-coated dishes in mTESR1(StemCell Technologies catalog #85850), mTESR1 Plus (StemCell Technologies catalog #100-0276), or mTESR1 with FGF2-DISCs (StemCultures LLC, catalog # DSC500). HUVECs (Lonza) were cultured on 0.2% gelatin coated tissue culture dishes in EGM-2 medium (PromoCell catalog #C-22111). HUVECs were used at passage 4 or 5. The hESC line used in this work was WMC-2 carrying a VE-cadherin-promotor-mOrange reporter.⁴⁶ (link) hiPSC lines used in this work to validate finding in different cell lines from various donors were as follows: TCW1E33–1C6,⁷⁴ (link) TCW1E44-b,⁷⁴ (link) TCW2E33–2E3,⁷⁴ (link) TCW2E44–4B4,⁷⁴ (link) TCW3E33-RC1H,⁷⁴ (link) TCW4E33-MC2C,⁷⁴ (link) TCW4E44-RC2C,⁷⁴ (link) GIH-161,⁷⁵ (link) F11350,⁷⁵ (link) F12442,⁷⁶ (link) F11430,⁷⁶ (link) F12436,⁷⁶ (link) F12424,⁷⁶ (link) and CO0002.⁷⁷ (link)

Bertucci T., Kakarla S., Winkelman M.A., Lane K., Stevens K., Lotz S., Grath A., James D., Temple S, & Dai G. (2023). Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells. APL Bioengineering, 7(3), 036107.

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Isolation and Characterization of iPSC-Derived Extracellular Vesicles

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The iPSCs (GD01-009) were gifted from Guidon Pharmaceutics (Beijing, China) and maintained in mTeSR1 plus (100-0276, Stemcell Technologies, Vancouver, BC, Canada) on Matrigel-coated plates (354277, Corning, NY, USA). The iPSCs were passaged as clumps using Versene Solution (15040066, Thermo Fisher Scientific, Watham, MA, USA) at a ratio of 1:4–1:6 every 4–5 days. The culture medium was substituted daily with GDEV medium (GDSJ0040, Guidon Pharmaceutics, Beijing, China) and centrifuged after daily collection to remove larger particles [42 (link)]. The supernatant was filtered and concentrated with Amicon Ultra-15 Centrifugal Filters (Ultracel-100 kDa, Merck Millipore, Burlington, MA, USA). The collected iPSC-EVs were stored at −80 °C.
The diameter, particle number and surface markers (CD9 or CD63) of iPSC-EVs were analyzed using a Flow NanoAnalyzer (NanoFCM, Xiamen, China). Transmission electron microscopy was used to identify the morphology of iPSC-EVs.

Deng P., Wang L., Zhang Q., Chen S., Zhang Y., Xu H., Chen H., Xu Y., He W., Zhang J, & Sun H. (2022). Therapeutic Potential of a Combination of Electroacupuncture and Human iPSC-Derived Small Extracellular Vesicles for Ischemic Stroke. Cells, 11(5), 820.

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Derivation and Characterization of iPSCs

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iPSCs were derived from primary fibroblasts of subject 1 (p.Leu537Pro) reprogrammed by using the non-integrating episomal technology (Epi5 Episomal iPSC Reprogramming Kit, Thermofisher Scientific), following manufacturer’s instructions. Control iPSCs were derived from fibroblasts of an age- and sex-matched healthy subject using the same experimental approach. Pluripotency of the obtained iPSCs was verified by assessing positivity for alkaline phosphatase activity (86R, Leukocyte Alkaline Phosphatase Kit, Sigma Aldrich) and SOX2 (14-9811-82, Invitrogen, 1:100), POU5F1/OCT4 (MA5-14845, Thermofisher, 1:400), SSEA4 (sc-21 704, Santa Cruz, 1:250), PODXL/TRA-1-60 (sc-21 705, Santa Cruz, 1:100) expression by immunofluorescence microscopy analysis. iPSC lines were grown in feeder-free condition using Matrigel (BD Biosciences) in mTeSR1 Plus (Stemcell Technologies) at 37°C, 5% CO₂. Differentiation of iPSC lines into motoneurons was performed as previously reported (54 ).

Flex E., Albadri S., Radio F.C., Cecchetti S., Lauri A., Priolo M., Kissopoulos M., Carpentieri G., Fasano G., Venditti M., Magliocca V., Bellacchio E., Welch C.L., Colombo P.C., Kochav S.M., Chang R., Barrick R., Trivisano M., Micalizzi A., Borghi R., Messina E., Mancini C., Pizzi S., De Santis F., Rosello M., Specchio N., Compagnucci C., McWalter K., Chung W.K., Del Bene F, & Tartaglia M. (2022). Dominantly acting KIF5B variants with pleiotropic cellular consequences cause variable clinical phenotypes. Human Molecular Genetics, 32(3), 473-488.

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