Log In Sign up
The largest database of trusted experimental protocols

Optima lc ms grade solvents

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Optima LC/MS grade solvents are high-purity solvents specifically designed for use in liquid chromatography-mass spectrometry (LC/MS) applications. These solvents are produced to meet the stringent requirements of LC/MS analysis, ensuring low levels of contaminants and impurities that could interfere with sensitive mass spectrometry detection.

Automatically generated - may contain errors

Lab products found in correlation

Acs grade, by Thermo Fisher Scientific (6 mentions) Yeast protein extract, by Promega (4 mentions) Acetonitrile, by Thermo Fisher Scientific (3 mentions) Ms grade enzymes, by Promega (2 mentions) Tris base, by Merck Group (2 mentions) Hydrochloride acid, by Merck Group (2 mentions) Dithiothreitol (dtt), by Promega (2 mentions) Reagent grade formic acid, by Merck Group (2 mentions) Ms grade trypsin lys c mix, by Promega (2 mentions) Trifluoroacetic acid, by Merck Group (2 mentions) Iodoacetamide, by Merck Group (2 mentions) Urea, by Merck Group (2 mentions) D asp3 lr, by Enzo Life Sciences (2 mentions) Nodularin, by Enzo Life Sciences (2 mentions) Dl dithiothreitol, by Merck Group (2 mentions) Ethylenediaminetetraacetic acid edta free protease inhibitor cocktail, by Merck Group (2 mentions) 13c6 leucine, by Cambridge Isotopes (2 mentions) Lc ms grade water, by Thermo Fisher Scientific (2 mentions) Dimethyl pimelimidate dihydrochloride dmp, by Merck Group (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Optima grade (92 citations) LC-MS grade (75 citations) Optima LC/MS (67 citations) Optima grade solvents (6 citations) LC-MS solvents (5 citations)

22 protocols using optima lc ms grade solvents

1

Synthesis of 5-plex mdDiLeu Labels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heavy isotopic reagents used for the synthesis of 5-plex mdDiLeu labels were purchased from Isotec (Miamisburg, OH). ACS grade and Optima LC/MS grade solvents were purchased from Fisher Scientific (Pittsburgh, PA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). Mass spec grade trypsin/Lys C mix and yeast protein extract were purchased from Promega (Madison, WI).
Zhong X., Frost D.C, & Li L. (2019). High-Resolution Enabled 5-plex Mass Defect-based N,N-Dimethyl Leucine Tags for Quantitative Proteomics. Analytical chemistry, 91(13), 7991-7995.
+ Open protocol
+ Expand
2

Isotope Labeling Reagent Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heavy isotopic reagents used for the synthesis of labels were purchased from Isotec (Miamisburg, OH). Yeast protein extract and MS-grade enzymes were purchased from Promega (Madison, WI). ACS grade and Optima LC/MS grade solvents were purchased from Fisher Scientific (Pittsburgh, PA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
Frost D.C., Rust C.J., Robinson R.A, & Li L. (2018). Increased N,N-Dimethyl Leucine Isobaric Tag Multiplexing by a Combined Precursor Isotopic Labeling and Isobaric Tagging Approach. Analytical chemistry, 90(18), 10664-10669.
+ Open protocol
+ Expand
3

Recombinant FGF2 and S117A-FGF2 Purification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant rat FGF2 and S117A-FGF2 were produced in-house using plasmids described in (Bailly et al. 2000 (link); Koleini et al. 2017 (link)). Histidine Tag at the N-terminal of the fusion proteins was used to purify the recombinant proteins using Nickel-sepharose (Ni-sepharose, high performance from GE healthcare, # 17-5268-01), according to the manufacturers protocols. FGFR1 antibody (anti-pY766-FGFR1) was purchased from SantaCruz (#sc-16309). The phospho (P) ERK (#9101), ERK (#9102), AKT (#9272), P-AKT (#9271), P38 (#9212) and P-P38 (#9211S) antibodies were purchased from Cell Signaling Technology. Optima LC/MS grade solvents were obtained from Fisher Scientific (Ottawa, Ontario, Canada). 1,2-Dinonanoyl-sn-glycero-3-phosphocholine (09:0 PC) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Butylated hydroxytoluene (BHT) was purchased from Sigma-Aldrich (Oakville, Ontario, Canada).
Koleini N., Nickel B.E., Edel A.L., Fandrich R.R., Ravandi A, & Kardami E. (2018). Non-mitogenic FGF2 protects cardiomyocytes from acute doxorubicin-induced toxicity independently of the protein kinase CK2/heme oxygenase-1 pathway. Cell and Tissue Research, 374(3), 607-617.
+ Open protocol
+ Expand
4

Synthesis of 4-plex DiLeu Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols
All isotopic reagents required for 4-plex DiLeu reagents synthesis were purchased from ISOTEC Inc (Miamisburg, OH). Urea, Tris base, hydrochloride acid (HCl), trifluoroacetic acid (TFA), reagent-grade formic acid (FA), iodoacetamide (IAA), triethylammonium bicarbonate (TEAB), N,N-dimethylformamide (DMF), 4-(4,6-dimethoxy1,3,5-triazin-2-yl)-4-methylmorpholinium tetrafluoroborate (DMTMM), N-methylmorpholine (NMM), and hydroxylamine solution were purchased from Sigma-Aldrich (St. Louis, MO). MS grade trypsin/Lys-C mix and dithiothreitol (DTT) were purchased from Promega (Madison, WI). All ACS grade and Optima LC/MS grade solvents were acquired from Fisher Scientific (Pittsburgh, PA).
Yu Q., Zhong X., Chen B., Feng Y., Ma M., Diamond C.A., Voeller J.S., Kim M., DeSantes K.B., Capitini C.M., Patel N.J., Hoover-Regan M.L., Burke M.J., Janko K., Puccetti D.M., Ikonomidou C, & Li L. (2020). Isobaric labeling strategy utilizing 4-plex N,N-dimethyl leucine (DiLeu) tags reveals proteomic changes induced by chemotherapy in cerebrospinal fluid of children with B-cell acute lymphoblastic leukemia. Journal of proteome research, 19(7), 2606-2616.
+ Open protocol
+ Expand
5

Isotope Labeling Reagent Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Heavy isotopic reagents used for the synthesis of labels were purchased from Isotec (Miamisburg, OH). Yeast protein extract and MS-grade enzymes were purchased from Promega (Madison, WI). ACS grade and Optima LC/MS grade solvents were purchased from Fisher Scientific (Pittsburgh, PA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).
Frost D.C., Buchberger A.R, & Li L. (2017). Mass Defect-Based Dimethyl Pyrimidinyl Ornithine (DiPyrO) Tags for Multiplex Quantitative Proteomics. Analytical chemistry, 89(20), 10798-10805.
+ Open protocol
+ Expand
6

Analytical Standards for Cyanotoxin Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Water, acetonitrile, methanol, acetic acid, and formic acid were all Optima LC/MS grade solvents and purchased from Fisher Scientific (Tewksbury, MA, USA). Microcystin standards D-Asp3-RR, MC-RR, YR, HtyR, LR, D-Asp3-LR, HilR, WR, LA, LY, LW, and LF, as well as nodularin and cylindrospermopsin were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Microcystin Dha7-LR was purchased from National Research Council Canada (Ottawa, ON, Canada). Microcystin Leu1-LR was purchased from GreenWater Laboratories (Palatka, FL, USA). D-Asp3-E-Dhb7-RR was purchased from Sigma Aldrich. Anabaenopeptin A and B, oscillamide Y, oscillaginin A, microginin 690 methyl ester, aeruginosamide B and C, cyanopeptolin 1040 MB and 1007, and micropeptin 1106 were purchased from MARBIONC (Wilmington, NC, USA). Anabaenopeptin F was synthetically created by the Stockdill laboratory at Wayne State University (Detroit, MI, USA). 15N MC-LR was purchased from Cambridge Isotope Laboratories, INC. (Tewksbury, MA, USA).
Shahmohamadloo R.S., Rudman S.M., Clare C.I., Westrick J.A., Wang X., De Meester L, & Fryxell J.M. (2023). Intraspecific genetic variation is critical to robust toxicological predictions of aquatic contaminants. bioRxiv.
+ Open protocol
+ Expand
7

Microcystin quantification using LC-MS and ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Water, acetonitrile, methanol, acetic acid, and formic acid were all Optima LC–MS grade solvents purchased from Fisher Scientific (Tewksbury, MA, USA). Additionally, 2-mercaptoethanol was purchased from Sigma Aldrich (St. Louis, MO, USA). MCs MC-LR, RR, YR, WR, HtyR, HilR, [d-Asp3]-RR, [d-Asp3]-LR, LA, LF, LY, and LW, as well as nodularin, were purchased from Enzo Life Sciences, Inc. (Farmingdale, NY, USA). The surrogate C2D5 MC-LR was purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). The Microcystins-Adda ELISA kit was purchased from Abraxis, Inc. (Warminster, PA, USA). Standards were prepared in methanol and diluted in LC–MS grade water. Samples were stored on ice after collection and until arrival to the laboratory where they were then frozen at −4 °C. Samples then went through a series of three freeze–thaw cycles before analysis [31 (link),39 (link),40 (link)].
Birbeck J.A., Peraino N.J., O’Neill G.M., Coady J, & Westrick J.A. (2019). Dhb Microcystins Discovered in USA Using an Online Concentration LC–MS/MS Platform. Toxins, 11(11), 653.
+ Open protocol
+ Expand
8

Glycoproteomic Analysis of PANC-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dithiothreitol (DTT), sequencing grade trypsin were purchased from Promega. Concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA120), iodoacetamide (IAA), acetyl-D18 glucosamine, D-lactose, methyl α-D-mannopyranoside, and manganese dichloride were obtained from Sigma-Aldrich. Tris base, urea (UA), sodium chloride, ammonium bicarbonate (ABC), calcium chloride (CaCl2), and ACS-grade and Optima LC/MS-grade solvents were obtained from Fisher Scientific. Formic acid (FA), 10% sodium dodecyl sulfate solution (SDS), trifluoroacetic acid (TFA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich. C18 OMIX tips and Phenylboronic acid (PBA) solid-phase extraction cartridges were obtained from Agilent. Hydrophilic interaction chromatography material (PolyHYDROXYETHYL A) was obtained from PolyLC. Microcon filters YM-30 (30 kDa) and amicon Ultra-0.5 ml centrifugal filters (10 kDa) were purchased from Merck Millipore. PANC-1 pancreatic ductal adenocarcinoma cells were from ATCC.
Chen Z., Yu Q., Yu Q., Johnson J., Shipman R., Zhong X., Huang J., Asthana S., Carlsson C., Okonkwo O, & Li L. (2021). In-depth Site-specific Analysis of N-glycoproteome in Human Cerebrospinal Fluid and Glycosylation Landscape Changes in Alzheimer's Disease. Molecular & Cellular Proteomics : MCP, 20, 100081.
+ Open protocol
+ Expand
9

Isotopic Labeling and Immunoprecipitation Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents and solvents were liquid chromatography (LC)/mass spectrometry (MS)-grade quality unless otherwise noted. [13C6]-leucine was obtained from Cambridge Isotope Laboratories (Andover, MA). Anti-FXN recombinant rabbit monoclonal antibody (mAb) EPR21840 (cat. # ab219414) was from Abcam (Cambridge, UK). Ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail, DL-dithiothreitol (DTT), EDTA-free Easypack protease inhibitor cocktail tablets, and dimethyl pimelimidate dihydrochloride (DMP) were purchased from MilliporeSigma (Billerica, MA). LC-grade water and acetonitrile were obtained from Burdick and Jackson (Muskegon, MI). Protein G Dynabeads for immunoprecipitation (IP) and radioimmunoprecipitation assay (RIPA) lysis buffer with EDTA were from ThermoFisher Scientific (Waltham, MA). LC/MS grade water and Optima LC/MS grade solvents were from Fisher Scientific (Pittsburgh, PA).
Rojsajjakul T., Selvan N., De B., Rosenberg J.B., Kaminsky S.M., Sondhi D., Janki P., Crystal R.G., Mesaros C., Khanna R, & Blair I.A. (2023). Expression and processing of mature human frataxin after gene therapy in mice. Research Square.
+ Open protocol
+ Expand
10

Isotopic Labeling and Immunoprecipitation Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
All reagents and solvents were liquid chromatography (LC)/mass spectrometry (MS)-grade quality unless otherwise noted. [13C6]-leucine was obtained from Cambridge Isotope Laboratories (Andover, MA). Anti-FXN recombinant rabbit monoclonal antibody (mAb) EPR21840 (cat. # ab219414) was from Abcam (Cambridge, UK). Ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail, DL-dithiothreitol (DTT), EDTA-free Easypack protease inhibitor cocktail tablets, and dimethyl pimelimidate dihydrochloride (DMP) were purchased from MilliporeSigma (Billerica, MA). LC-grade water and acetonitrile were obtained from Burdick and Jackson (Muskegon, MI). Protein G Dynabeads for immunoprecipitation (IP) and radioimmunoprecipitation assay (RIPA) lysis buffer with EDTA were from ThermoFisher Scientific (Waltham, MA). LC/MS grade water and Optima LC/MS grade solvents were from Fisher Scientific (Pittsburgh, PA).
Rojsajjakul T., Selvan N., De B., Rosenberg J.B., Kaminsky S.M., Sondhi D., Janki P., Crystal R.G., Mesaros C., Khanna R, & Blair I.A. (2024). Expression and processing of mature human frataxin after gene therapy in mice. Scientific Reports, 14, 8391.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!