Secondary antibodies coupled to horseradish peroxidase

Anti α-L-fucosidase (FUCA1) is a polyclonal antibody from Proteintech Group (Rosemont, IL, USA) (16420-1-AP) which recognizes the α-L-fucosidase-1 protein. Anti-turbo-GFP-tag is a monoclonal antibody from Origene Technologies (TA150041-100) which recognizes over expressed recombinant proteins containing the turbo-GFP tag fused to either the amino- or carboxy-termini of targeted proteins. Monoclonal anti-α-tubulin (#T9026) was from Sigma Aldrich (St Louis, MO, USA). Secondary antibodies coupled to horseradish peroxidase were from Amersham Pharmacia Biotech (Piscataway, NJ, USA). Monoclonal anti-α-tubulin (#T9026) was from Sigma Aldrich (St Louis, MO, USA). Secondary antibodies coupled to horseradish peroxidase were from Amersham Pharmacia Biotech (Piscataway, NJ, USA).

Anti Shh (#2207) and anti Gli1 (#2553) are rabbit monoclonal antibodies from Cell Signaling Technology. Anti Smo (E-5) sc-166685 (#C1315) mouse monoclonal antibody and anti Gli2 (H-300) sc-28674 (#K2013) rabbit polyclonal antibody are from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal antibody anti-Parp (#9542) is from Cell Signaling Technology. Monoclonal anti-tubulin (#T9026) is from Sigma-Aldrich (St Louis, MO, USA). Secondary antibodies coupled to horseradish peroxidase are from Amersham Pharmacia Biotech (Piscataway, NJ, USA).

Primary antibodies used for Western blot analysis and immunohistochemistry: mouse monoclonal anti-Ki67 (clone MIB-1) (Dako, Glostrup, Denmark), mouse monoclonal anti-cyclin A and anti-cyclin E (Invitrogen, Carlsbad, CA), mouse monoclonal anti-p27 and anti-p21 (Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal anti-tubulin (Sigma-Aldrich, St Louis, MO), rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2), anti-p44/42 MAPK (Erk1/2), anti-phospho-Akt, anti-Akt, anti-phospho-c-Jun, anti-c-Jun, anti-phospho-c-Fos, anti-c-Fos, anti-phospho-CREB, anti-CREB (Cell Signaling Technology, Danvers, MA). Secondary antibodies coupled to horseradish peroxidase were obtained from Amersham Pharmacia Biotech (Diegem, Belgium). Palbociclib (PD-0332991) was purchased at Selleck Chemicals (Munich, Germany).