Cell sorting protocols were as previously described [28 (link), 32 (link)]. Briefly, bone marrow was flushed in PBS supplemented with 0.1% bovine serum albumin and 2 mM EDTA, filtered through 40 μm cell strainers, and subjected to red blood cell lysis in ACK buffer (0.15 M NH4Cl, 10 mM KHCO3, 0.1 mM EDTA). The samples were stained with biotin anti-mouse Lineage Panel (BioLegend), PE-Cy7 cKit (clone 2B8, BioLegend), APC-Cy7 Sca-1 (D7, BioLegend), FITC CD48 (HM48-1, eBioscience), APC CD150 (TC15-12F12.2, BioLegend), PE Flt3 (A2F10, eBioscience), Brilliant Violet 421 CD34 (RAM34, BD Biosciences), and streptavidin-PECy5 (BioLegend). Cell sorting was performed on FACSAria, with the FACS Diva software (BD Biosciences).
Fitc cd48 hm48 1
Manufactured by Thermo Fisher Scientific
FITC CD48 (HM48-1) is a fluorescently labeled antibody used for the detection and analysis of CD48 expression on cell surfaces. CD48 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein involved in cell-cell adhesion and immune regulation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc cd48 hm48 1
1
Isolation and Sorting of Hematopoietic Stem Cells
2
Mouse Hematopoietic Stem Cell Isolation
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Mouse bone marrow was flushed from femurs, tibiae, humeri, and ilia of mice with a 26-G needle in PBS supplemented with 0.1% BSA and 2 mM EDTA, filtered through 40-μm cell strainers, and subjected to red blood cell lysis in ACK buffer (0.15 M NH4Cl, 10 mM KHCO3, and 0.1 mM EDTA). The cells were stained with Biotin anti-mouse Lineage Panel (BioLegend) and lineage-positive cells depleted using biotin-binding Dynabeads (Life Technologies). The lineage-negative fraction was stained with PeCy7-cKit/CD117 (clone 2B8, BD Biosciences), APC-Sca1 (E13–161.7, BioLegend), FITC-CD48 (HM48-1, eBioscience), PE-CD150 (mShad150, eBioscience), and streptavidin-PECy5 (BioLegend). Propidium iodide or DAPI was added immediately before sorting for dead cell exclusion. Cell sorting was performed on the FACSAria instrument and was analyzed with FACS Diva software (BD Biosciences).
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