Lc ms 2010a

Manufactured by Shimadzu

Sourced in Japan

The Shimadzu LC-MS 2010A is a liquid chromatography-mass spectrometry (LC-MS) system designed for qualitative and quantitative analysis of chemical compounds. It combines the separation capabilities of liquid chromatography with the detection and identification capabilities of mass spectrometry. The LC-MS 2010A is capable of performing accurate mass measurements and can be used for a wide range of applications in various industries, such as pharmaceutical, environmental, and food analysis.

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Lab products found in correlation

Av 400 spectrometer, by Bruker (3 mentions) Advance 3 300, by Bruker (2 mentions) Avance 400, by Bruker (2 mentions) Fluoromax 4 spectrophotometer, by Horiba (2 mentions) Tlc silica gel plates 60 f254, by Merck Group (1 mentions) Avance 400 mhz spectrometer, by Bruker (1 mentions) F 4500 fluorescence spectrofluorometer, by Hitachi (1 mentions) Uh5300 spectrophotometer, by Hitachi (1 mentions) Flexanalysis, by Bruker (1 mentions) 400 mhz spectrometer, by Bruker (1 mentions) Lambda 365 uv vis spectrophotometer, by PerkinElmer (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Spectrum two ft ir spectrometer, by PerkinElmer (1 mentions) Ft ir4100, by Shimadzu (1 mentions) Av 400 nmr spectrometer, by Bruker (1 mentions) Tcs sp5 microscope, by Leica (1 mentions) Lc 2010a, by Shimadzu (1 mentions) Spd m10a, by Shimadzu (1 mentions) Lc 10advp, by Shimadzu (1 mentions) Cto 10ac, by Shimadzu (1 mentions)

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LC-2010A (40 citations)

28 protocols using lc ms 2010a

Synthesis and Characterization of JEZ-C

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JEZ-C [3,4,5-trihydroxy-N-{4-[(5-methylisoxazol-3-yl)sulfamoyl]phenyl}benzamide; Fig. 1A] was prepared from GA and SMZ, as outlined in Fig. 1B. Subsequently, an appropriate amount of distilled water was added to the mixture, and the raw precipitated product was separated by vacuum filtration. The raw product was then recrystallized in a tetrahydrofuran-methanol solvent system. Electrospray ionization mass spectrum (ESI-MS) was detected on a Shimadzu LC-MS 2010A. 1H and 13C NMR spectra were assessed by using a Bruker Advance III 300 at 400 and 125 MHz, respectively.
JEZ-C has the following properties: White powder, mp: 217–218°C, yield 63%, MS-ESI: 404.0[M-H]⁻, ¹H-NMR (400 MHz, DMSO-d6) δ 11.33 (s, 1H, -SO₂-NH), 10.29 (s, 1H, -CO-NH), 7.95–7.77 (m, 4H, 4xAr-H), 6.94 (s, 2H, 2xAr-H), 6.13 (s, 1H, isoxazol-H), 2.28 (s, 3H, -CH₃). ¹³C-NMR (125 MHz, DMSO-d6) δ170.30, 166.09, 157.64, 145.60, 144.06, 137.37, 132.96, 127.83, 124.31, 119.79, 107.47, 95.43, 12.10.

Huang L., Jin P., Lin X., Lin C., Zheng L, & Zhao J. (2017). Beneficial effects of sulfonamide-based gallates on osteoblasts in vitro. Molecular Medicine Reports, 15(3), 1149-1156.

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Electrospray Ionization Mass Spectrometry

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A steel capillary from austentic stainless steel (AISI 316 L) of 8 mm length with an inner diameter of 50 μm and an outer diameter of 110 ± 10 μm (Holdenrieder, Germany) was inserted into a microfluidic channel of 80 μm height and 100 μm width. The PDMS chip was bonded to another PDMS slice instead of glass. Before usage, the inner surface of the chip and the capillary were hydrophobized by flushing with rain-x and isopropanol. The chip was fixed on a xyz-micromanipulator and a PTFE tubing was brought close to the protruding capillary end for oil drainage. The capillary was contacted by a metal wire to apply 1.5–3 kV for electrospray ionization. Opposite to the capillary a grounded metal plate was positioned functioning as counter electrode. This setup was placed perpendicular to the orifice of a mass spectrometer (Shimadzu LCMS-2010A, Germany) from which the ESI source was removed before. The mass spectrometer was operated in selected ion mode ([M + H]⁺ at m/z = 582.5) with an event time of 0.025 s (40 Hz). Perfluorodecalin was used as continuous oil phase, as it is not disturbing the generation of an electrospray at the applied voltage.

Mahler L., Wink K., Beulig R.J., Scherlach K., Tovar M., Zang E., Martin K., Hertweck C., Belder D, & Roth M. (2018). Detection of antibiotics synthetized in microfluidic picolitre-droplets by various actinobacteria. Scientific Reports, 8, 13087.

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Purification and Characterization of Compounds

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We used commercial reagents and solvents without further purification. We also performed all reactions in the air atmosphere unless otherwise stated. Reactions were monitored by thin-layer chromatography (TLC) performed on Merck TLC Silica gel plates (60 F254), using a UV light for visualization and basic aqueous potassium permanganate or iodine fumes as a developing agent. ¹H and ¹³C NMR spectra were recorded on Bruker Avance 400 instrument with operating frequency of 400 and 100 MHz, respectively, and calibrated using residual undeuterated chloroform (δH = 7.28 ppm) and CDCl₃ (δC = 77.16 ppm) or undeuterated dimethyl sulfoxide (DMSO) (δH = 2.50 ppm) and DMSO-d₆ (δC = 39.51 ppm) as internal references. The following abbreviations are used to set multiplicities: s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet, br = broad. The purity of the final compounds was checked by liquid chromatography-mass spectrometry (LCMS) in a Shimadzu LCMS-2010A using three types of detection systems such as EDAD, ELSD, and UV and was found to be at least 95%.

Curreli F., Ahmed S., Benedict Victor S.M., Iusupov I.R., Belov D.S., Markov P.O., Kurkin A.V., Altieri A, & Debnath A.K. (2020). Preclinical Optimization of gp120 Entry Antagonists as anti-HIV‑1 Agents with Improved Cytotoxicity and ADME Properties through Rational Design, Synthesis, and Antiviral Evaluation. Journal of medicinal chemistry, 63(4), 1724-1749.

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Spectroscopic Characterization of Compounds

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¹H NMR spectra were collected with a Bruker Avance 400 MHz spectrometer using DMSO-d₆ as solvent. ESI-mass spectra (MS) were recorded with a LC-MS 2010A (Shimadzu) instrument. High-resolution MALD-TOF MS were collected with a Bruker Daltonics Flex-Analysis. Fluorescence spectra were measured on a Hitachi F-4500 fluorescence spectrofluorometer (Kyoto, Japan). UV–Vis spectra were collected with a UH5300 spectrophotometer (HITACHI, Japan).

Wei Y., Hu X., Shen L., Jin B., Liu X., Tan W, & Shangguan D. (2017). Dicyanomethylene Substituted Benzothiazole Squaraines: The Efficiency of Photodynamic Therapy In Vitro and In Vivo. EBioMedicine, 23, 25-33.

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Spectroscopic Characterization of Metal Complexes

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All the raw materials including reagents, catalysts, solvents and drying agents were of fine chemical grade and utilized as received by the vendor. The MTT [2-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazol-3-ium bromide] dye was purchased from Sigma, St. Louis, MO, USA.
Infrared (IR) spectra of the synthesized materials were recorded in the range of 4000–550 cm⁻¹ with PerkinElmer Spectrum Two FT–IR spectrometer. By using TMS as internal reference compound, ¹H and ¹³C NMR spectra were recorded in DMSO‑d₆ solvent on JEOL 400 MHz and Bruker 400 MHz spectrometer at room temperature. Using ThermoQuest Elemental Analyzer, elemental analyses of the compounds was carried out. Electronic spectra of ligands and their metal complexes were recorded on a PerkinElmer LAMBDA 365 UV–Vis spectrophotometer in the range of 1100–200 nm. Thermal analysis of the metal complexes was carried out with an SDT Q600 analyzer, by increasing the temperature from RT to 1000 °C at the rate of 10 °C min⁻¹. The molar conductance measurements were made on ELICO CM 180 conductivity meter with a cell constant of 1.0 after calibration with standard KCl solution at 25 °C. LC–ESI–MS spectra were recorded on LCMS 2010A, SHIMADZU instrument.

Kumbar M., Patil S.A., Toragalmath S.S., Kinnal S.M., Shettar A, & Hosakeri J.H. (2020). Anticancer activity studies of novel metal complexes of ligands derived from polycyclic aromatic compound via greener route. Journal of Organometallic Chemistry, 914, 121219.

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Synthesis and Characterization of Novel Dyes

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All chemicals used in this study were prepared from Sigma-Aldrich (St. Louis, MO, USA), Fluka (Neu-Ulm, Germany) and Merck (Darmstadt, Germany) companies. The progress of the reactions was tracked by thin-layer chromatography (TLC) using aluminum plates coated by silica gel 60 F254. The FT-IR spectra were provided using Shimadzu FT-IR4100 setup through preparing the samples by KBr tablet. The UV-vis for the synthesized dyes were obtained via the Rayleigh UV-1800 setup. The melting points of the dyes (D1–D10) were measured using IA9000 electric melting apparatus. A Bruker (DRX-500 Avanes) NMR was used to record the ¹H NMR and ¹³C NMR spectra (ESI†). All NMR spectra were determined in DMSO at room temperature. The molecular weight of some products D1–D10 were determined by using LC-MS technique. LC-MS analysis was performed on a Shimadzu LCMS 2010 A by using positive electrospray ionization.

Davasaz Rabbani M.A., Khalili B, & Saeidian H. (2020). Novel edaravone-based azo dyes: efficient synthesis, characterization, antibacterial activity, DFT calculations and comprehensive investigation of the solvent effect on the absorption spectra. RSC Advances, 10(59), 35729-35739.

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LC-MS Analysis of COE Compound

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The following conditions were maintained in running the sample on LC-MS 2010A (Shimadzu Japan). The C18 column was used as a stationary phase and a 90:10 v/v ratio of methanol: water was used (flow rate of 200 μL min⁻¹) as the mobile phase. The COE was dissolved in the mobile phase and injected (volume 5 μL) and absorbance was recorded at 254 nm.

Patil P.P., Patil V.S., Khanal P., Darasaguppe H.R., Charla R., Bhatkande A., Patil B.M, & Roy S. (2022). Network pharmacology and in vitro testing of Theobroma cacao extract’s antioxidative activity and its effects on cancer cell survival. PLoS ONE, 17(4), e0259757.

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Analytical Characterization of Chemical Compounds

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All chemicals in this work were obtained commercially and used without further purification. High resolution mass spectra (HRMS) were recorded on a LC-MS 2010A (Shimadzu) instrument. ¹H and ¹³C NMR data were acquired on a Bruker AV-400 NMR spectrometer. Absorption data were measured on a UV-3101PC spectrophotometer. Fluorescence data were obtained on a Horiba FluoroMax-4 spectrophotometer. Fluorescence imaging of HOCl in live RAW 264.7 macrophage cells was performed on a Leica TCS SP5 microscope.

Li X., Wu L., Zhao Z., Liu C, & Zhu B. (2019). A novel highly specific and ultrasensitive fluorescent probe for monitoring hypochlorous acid and its application in live cells. RSC Advances, 9(8), 4659-4664.

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LC-MS Analysis of Oxysterols

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The LC-MS was a model LC 2010A (Shimadzu Scientific Instruments, Columbia, MD) equipped with two LC-10ADvp pumps, a low volume high pressure mixer, a SIL-HT autosampler, a CTO-10AC column oven, a SPD-M10A photo-diode array (PDA) Detector, a LCMS-2010A mass spectrometer with APCI interface, drying gas and computer system with LabSolutions software.
A flow control valve (FCV) was installed between the PDA and MS to divert the system flow away from the MS detector while allowing continued PDA monitoring. The PDA range was 190–250 nm with the primary analysis channel set at 204 nm for cholesterol and stigmasterol detection.
The MS parameters were optimized for the detection of 24(S)-hydroxycholesterol with the follow settings: interface: APCI, ion mode: positive, detector voltage: 1.5 kV, APCI Interface temperature: 400°C, CDL temperature: 230°C, nebulizing gas flow: 2.5 L/min, heat block temperature: 200°C and sampling: 1.5625 Hz (time constant: 0.640 sec). Selected ion-monitoring (SIM) time segments were employed to maximize the sensitivity of the MS detector as each cluster of chromatographic peaks eluted from the column.

Narayanaswamy R., Iyer V., Khare P., Bodziak M.L., Badgett D., Zivadinov R., Weinstock-Guttman B., Rideout T.C., Ramanathan M, & Browne R.W. (2015). Simultaneous Determination of Oxysterols, Cholesterol and 25-Hydroxy-Vitamin D3 in Human Plasma by LC-UV-MS. PLoS ONE, 10(4), e0123771.

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Synthesis and Characterization of JEZ-C Compound

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Electrospray ionization mass spectrum (ESI-MS) was recorded on a Shimadzu LC-MS 2010A. ¹H and ¹³C NMR spectra were obtained from a Bruker Advance III 300 at 400 and 125 MHz, respectively.
3,4,5-Trihydroxy-N-[4-(thiazol-2-ylsulfamoyl)-phenyl]-benzamide (JEZ-C) was prepared from GA and Sulfamethoxazole with the same procedure in previous studies [18 (link)]. The synthetic route is presented in Fig 1 in detail. The purity of JEZ-C is greater than 95% by TLC (Thin Layer Chromatography, In three different development system only one spot appeared) and is 98% by HPLC (High Performance Liquid Chromatography) method.

Wei S., Lu Z., Zou Y., Lin X., Lin C., Liu B., Zheng L, & Zhao J. (2015). A Novel Synthesized Sulfonamido-Based Gallate—JEZ-C as Potential Therapeutic Agents for Osteoarthritis. PLoS ONE, 10(6), e0125930.

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