Trace 1310 gc

The extract was dried over anhydrous CaCl₂, and the essential oil was stored at 4 °C until the GC-MS/FID measurements. The yield of oil was 1.52%. The GC-MS analysis was carried out using a Thermo Scientific Trace GC 1310 connected to a Thermo TSQ 9610 MS system on a DB-5 capillary column (60 m × 0.25 mm, 0.25 mm film thickness) with helium as the carrier gas (0.8 mL/min). GC oven temperature was maintained at 80 °C for 10 min, programmed to 280 °C at a rate of 4 °C/min, and kept constant at 280 °C for 5 min. The split ratio was adjusted to 1:20. The injector temperature was set to 250 °C. The mass spectra were recorded at 70 eV. The mass range was m/z 35–650. GC-FID analysis was performed using a Thermo Scientific Trace GC 1310 instrument. The FID detector temperature was 280 °C. To obtain the same elution order as GC-MS, simultaneous auto-injection was performed in duplicate on the same column under the same operational conditions. The relative percentage of the separated compounds was calculated from the FID chromatograms [53 ,54 (link),55 (link)]. Alkanes were used as reference points in the calculation of Kovats Indices (KI). Compounds were identified by comparing their retention times and mass spectra with those obtained from authentic samples and/or the NIST and Wiley spectra, as well as literature data [54 (link),55 (link),56 ,57 (link)].

The essential oil was kept at 4 °C until the GC-MS/FID computations, and the extract was dried over anhydrous CaCl₂. The oil yield was 1.52%. On a DB-5 capillary column (60 m × 0.25 mm, 0.25 mm film thickness) with helium as the carrier gas (0.8 mL/min), GC-MS analysis was performed using a Thermo Scientific Trace GC 1310 linked to a Thermo TSQ 9610 MS system. After being held at 80 °C for 10 min, the GC oven’s temperature was scheduled to rise to 280 °C at a rate of 4 °C per minute, and it remained at 280 °C for 5 min. It was changed to a 1:20 split ratio. The injector was adjusted at a temperature of 250 °C. At a mass of 70 eV, the spectra were captured. The m/z 35–650 was the mass range. A Thermo Scientific Trace GC 1310 equipment was used to conduct the GC-FID analysis. The FID detector was held at a temperature of 280 °C. Parallel auto-injection was carried out twice on the same column with the same operating settings in order to achieve the same elution sequence as GC-MS. From the FID chromatograms, the relative proportion of the isolated chemicals was determined [29 (link)]. When calculating the Kovats Indices (KIs), alkanes were employed as reference points. Authentic samples, the NIST and Wiley spectra, as well as information from the literature, were used to compare retention durations and mass spectra, which helped identify the compounds [30 (link),31 ,32 (link)].

Total lipids from cortex, plasma and livers were extracted using Folch’s procedure [41 (link)]. Boron trifluoride in methanol was used for transmethylation [42 (link)]. Hexane was used to extract fatty acid methyl esters (FAMEs) and dimethyl acetals (DMAs). Analyses were performed on a GC Trace 1310 (Thermo Scientific, Illkirch, France) gas chromatograph (GC) using a CPSIL-88 column (100 m × 0.25 mm inside diameter, film thickness 0.20 μm; Agilent, CA, USA). This device was coupled to a flame ionization detector (FID). The configuration was: inlet pressure of hydrogen 210 kPa, oven temperature 60 °C for 5 min + 165 °C at 15 °C per min and upholding for 1 min, +225 °C at 2 °C per min and upholding at 225 °C for 17 min. The injector and the detector were maintained at 250 °C. Comparisons with commercial and synthetic standards enabled the identification of FAMEs and DMAs. The ChromQuest 5.0 version 3.2.1 software (Thermo Scientific, Illkirch, France) was used to process the data.