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Elite vectastain abc reagents

Manufactured by Vector Laboratories
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Elite Vectastain ABC reagents are a kit designed for the detection of antigens in biological samples. The kit includes an avidin-biotin complex (ABC) that binds to biotinylated secondary antibodies, enabling the visualization of target antigens. The reagents are suitable for use in various immunohistochemical and immunocytochemical techniques.

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Lab products found in correlation

Vector vip, by Vector Laboratories (5 mentions) Anti mouse antibodies, by Vector Laboratories (2 mentions) Silanized micro vial inserts, by Agilent Technologies (2 mentions) Fitc dextran, by Merck Group (2 mentions) Autosampler vial, by Thermo Fisher Scientific (2 mentions) Gfap d1f4q, by Cell Signaling Technology (1 mentions) Gapdh sc32233 antibody, by Santa Cruz Biotechnology (1 mentions) Anti mouse secondary antibodies, by Vector Laboratories (1 mentions) Bz x710, by Keyence (1 mentions) Biotinylated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Anti cox 2, by Abcam (1 mentions) Anti c fos, by Abcam (1 mentions) Bovine anti goat, by Santa Cruz Biotechnology (1 mentions) Inos antibody, by BD (1 mentions) Antibodies against gfap, by Cell Signaling Technology (1 mentions) Goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti synapsin 1, by Abcam (1 mentions) Anti synaptophysin, by Abcam (1 mentions) Cox 2 antibody, by Cayman Chemical (1 mentions) Anti mouse bovine horseradish peroxidase conjugated secondary antibodies, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

ABC reagent (242 citations) Vectastain ABC reagent (167 citations) Vectastain Elite ABC reagent (156 citations) ABC Elite (135 citations) Vectastain Elite ABC (104 citations) Vectastain ABC (102 citations) Vectastain Elite (56 citations) ABC Elite reagent (39 citations) Vectastain reagents (6 citations) Vectastain Elite Reagent (3 citations)

7 protocols using elite vectastain abc reagents

1

Immunohistochemical Analysis of APP and Aβ

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Elite Vectastain ABC reagents, Vector VIP (SK-4600), biotinylated anti-rabbit (BA-1000), and mouse on mouse anti-mouse (MKB-2225) antibodies were purchased from Vector Laboratories Inc. (Burlingame, CA). APP (Y188) ab32136 rabbit monoclonal antibody that reacts with both mouse and human protein was purchased from Abcam Inc. (Cambridge, MA). Aβ anti-rabbit monoclonal antibody specific to human (8243) was obtained from Cell Signaling Technology Inc. (Danvers, MA).
Floden A.M., Sohrabi M., Nookala S., Cao J.J, & Combs C.K. (2020). Salivary Aβ Secretion and Altered Oral Microbiome in Mouse Models of AD. Current Alzheimer research, 17(12), 1133-1144.
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2

Immunohistochemistry and Western Blot Analyses

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Antibodies were utilized for immunohistochemistry and western blot analyses. Primary antibodies against GFAP (D1F4Q) and Aβ (D54D2), PS1 (D39D1) and BACE (D10E5) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The Iba1 antibody (019-19741) was purchased from Wako Chemicals USA, Inc. (Richmond, VA, USA). The CD68 (MCA1957) antibody was purchased from Bio-Rad Laboratories, Inc. (Hercules, CA, USA). The anti-APP, Y188 (ab32136) antibody was purchased from Abcam (Cambridge, MA, USA). The GAPDH (sc32233) antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Elite Vectastain ABC reagents, Vector VIP, biotinylated anti-rabbit, and anti-mouse secondary antibodies were purchased from Vector Laboratories, Inc (Burlingame, CA, USA).
Sahu B., Mackos A.R., Floden A.M., Wold L.E, & Combs C.K. (2021). Particulate matter exposure exacerbates Aβ plaque deposition and gliosis in APP/PS1 mice. Journal of Alzheimer's disease : JAD, 80(2), 761-774.
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3

Quantitative Analysis of Catecholaminergic Neurons

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One set of tissue sections from each rat in the BNST DSAP experiment (with each set containing a complete rostrocaudal series of sections spaced by 210 μm) was incubated in a mouse monoclonal antiserum against DbH (1:50,000; Millipore, MAB308; AB_2245740) followed by biotinylated donkey anti-mouse IgG (1:500; Jackson ImmunoResearch). Sections were then treated with Elite Vectastain ABC reagents (Vector) and reacted with diamino-benzidine (DAB).
Sections through the cNTS containing the A2 cell group and VLM containing the A1 cell group (~15.2–14.15 mm caudal to bregma) were imaged at 20x using a Keyence microscope (BZ-X710). The total number of DbH+ neurons were counted bilaterally in each region and averaged per section. Sections through the pontine locus coeruleus (LC; ~10–10.2 mm caudal to bregma) were imaged at 10x. Due to extremely dense DbH labeling in the LC, it was not possible to visualize and accurately count individual DbH+ cells. Instead, a region of interest (ROI) encompassing the DbH labeling in LC was drawn using Image J bilaterally and the area of each ROI was measured and averaged across four LC ROIs (equivalent to bilateral LC in 2 tissue sections per rat).
Edwards C.M., Guerrero I.E., Thompson D., Dolezel T, & Rinaman L. (2024). An ascending vagal sensory-central noradrenergic pathway modulates retrieval of passive avoidance memory. bioRxiv.
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4

Biochemical Markers Quantification Protocol

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FITC-dextran (MilliporeSigma, Burlington, MA, USA), ELISA kits for TNF-α, IL-1β, IL-10, and lipocalin were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against GFAP, Aβ, BACE-1, and PSD-95 were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Anti-synaptophysin, anti-synapsin 1, anti-cFos, and anti-COX-2 antibodies were purchased from Abcam (Cambridge, MA, USA). Anti-Iba-1 antibody was purchased from Wako Chemicals USA, Inc. (Richmond, VA, USA). iNOS antibody was purchased from BD, Biosciences (San Jose, CA, USA). Anti-GAPDH, α-tubulin antibodies and horseradish peroxidase-conjugated secondary antibodies (goat anti-mouse IgM, goat anti-mouse IgG, goat anti-rabbit, goat anti-rat, bovine anti-goat and bovine anti-mouse were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Elite Vectastain ABC reagents, Vector VIP, biotinylated anti-rabbit, and anti-mouse antibodies were purchased from Vector Laboratories Inc (Burlingame, CA, USA). Standards used in mass spectroscopy analysis were purchased from MilliporeSigma (Burlington, MA, USA). Autosampler vials were obtained from ThermoFisher Scientific, (Waltham, MA, USA), silanized micro-vial inserts were from Agilent (Santa Clara CA; part #5181-8872) and inserts were from VWR (Radnor, PA, USA).
Kaur H., Nagamoto-Combs K., Golovko S., Golovko M.Y., Klug M.G, & Combs C.K. (2020). Probiotics ameliorate intestinal pathophysiology in a mouse model of Alzheimer’s disease. Neurobiology of aging, 92, 114-134.
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5

Antibody Characterization and Validation

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Anti-β-amyloid precursor protein (AβPP) and anti-occludin antibodies were purchased from Zymed Laboratories (San Francisco, CA). Anti-rabbit (goat), anti-goat (bovine), anti-rat (goat), and anti-mouse (bovine) horseradish peroxidase-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). CD36 and actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Elite Vectastain ABC reagents, Vector VIP, biotinylated anti-rabbit, anti-mouse, and anti-rat antibodies were purchased from Vector Laboratories Inc. (Burlingame, CA, USA). Anti-human and anti-mouse CD68 antibodies were purchased from AbD Serotec (Oxford, UK). BACE antibody was purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). COX-2 antibody was purchased from Cayman Chemical (Ann Arbor, MI, USA). Aβ clone 4G8 was purchased from Covance (Emeryville, CA, USA). Anti-amyloid beta precursor protein antibody (Y188) was purchased from Abcam (Cambridge, MA, USA). The PHF-1 anti-phosphoSer396/404 mouse monoclonal antibody was a generous gift from Dr. Peter Davies, Albert Einstein College of Medicine.
Puig K.L., Lutz B.M., Urquhart S.A., Rebel A.A., Zhou X., Manocha G.D., Sens M., Tuteja A.K., Foster N.L, & Combs C.K. (2015). Overexpression of Mutant Amyloid-β Protein Precursor and Presenilin 1 Modulates Enteric Nervous System. Journal of Alzheimer's disease : JAD, 44(4), 1263-1278.
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6

Antibody and ELISA Reagent Procurement

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Antibodies against GFAP and amyloid beta (Aβ) were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Anti-c-Fos antibody was purchased from Abcam (Cambridge, MA, USA). Anti-Iba-1 antibody was purchased from Wako Chemicals USA, Inc. (Richmond, VA, USA). Elite Vectastain ABC reagents, Vector VIP, biotinylated anti-rabbit, and anti-mouse antibodies were purchased from Vector Laboratories Inc (Burlingame, CA, USA). The Aβ 1–40 and Aβ 1–42 enzyme-linked immunosorbent assay (ELISA) kits were obtained from EMD Millipore (Burlington, MA). Standards used in mass spectroscopy analysis were purchased from Millipore-Sigma Burlington, MA, USA. Autosampler vials were obtained from ThermoFisher Scientific, (Waltham, MA, USA), Silanized micro-vial inserts from Agilent (Santa Clara CA; part #5181–8872) and inserts were from VWR (Radnor, PA, USA).
Kaur H., Golovko S., Golovko M.Y., Singh S., Darland D.C, & Combs C.K. (2020). Effects of probiotic supplementation on short chain fatty acids in the App NL-G-F mouse model of Alzheimer’s disease. Journal of Alzheimer's disease : JAD, 76(3), 1083-1102.
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7

Inflammatory Responses and Microbiome Analysis

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Antibiotics including vancomycin (V2002-5G), neomycin (N6386-100G), and pimaricin (P9703-25MG) were purchased from MilliporeSigma (Burlington, MA, USA). FITC-dextran was purchased from MilliporeSigma (Burlington, MA, USA), and ELISA kits for TNF-α, IL-1β, and IL-10 were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against GFAP and Aβ were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). The anti-Iba1 antibody was purchased from Wako Chemicals USA, Inc. (Richmond, VA, USA). Elite Vectastain ABC reagents, Vector VIP, biotinylated anti-rabbit, and anti-mouse antibodies were purchased from Vector Laboratories Inc (Burlingame, CA, USA). ZymoBIOMICS DNA Kits D4300 (Zymo, Irvine, CA, USA), A Quantibody® Mouse Cytokine Array (QAM-TH-17; RayBiotech, Inc., Norcross, GA, USA), and blood and chocolate agar plates (Thermo Fisher Scientific, Rockford, IL, USA) were also used. Ghost dyeTM Violet 510 was purchased from Tonbo Biosciences (San Diego, CA, USA). The antibodies and the clones used in flow cytometry experiments are detailed in Table 1.
Kaur H., Nookala S., Singh S., Mukundan S., Nagamoto-Combs K, & Combs C.K. (2021). Sex-Dependent Effects of Intestinal Microbiome Manipulation in a Mouse Model of Alzheimer’s Disease. Cells, 10(9), 2370.
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