Azd8055

Commercially available antibodies to PARP [9542 S], phospho-FOXO3a (Thr32) [9464 T], FOXO3a [2497 S], phospho-MDM2 (Ser166) [3521 S], Beclin1 [3495 S] and Bim [2933 S] were procured from Cell Signaling Technology, MA. Bax [SC-493], phospho-SGK1 (Ser422) [SC-28338], and p53 [SC-6243] antibodies were purchased from Santa Cruz Biotechnology, CA. HRP-conjugated antibody to actin [A3854], Z-VAD-fmk and chloroquine were purchased from Sigma-Aldrich, MO. Caspase 3 [NB100-56708] and LC3 [NB100-2220] antibody were purchased from Novus Biologicals, CO. Commercially available AZD5363 and AZD8055 were procured from Cayman Chemicals, MI.

Thapsigargin (Tg), puromycin dihydrochloride, p38 inhibitor (SB203580), and JNK inhibitor (SP600125) were purchased from Sigma (St. Louis, MO, USA). AZD8055 and refametinib were procured from Cayman Chemical (Ann Arbor, MI, USA). The anti-puromycin and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Millipore (Burlington, MA, USA) and Santa Cruz Biotechnology (Dallas, TX, USA), respectively. The anti-phospho 4E-BP1 (Ser65), anti-4E-BP1, anti-phospho ERK 1/2, anti-ERK 1/2, anti-phospho p38, anti-p38, anti-phospho-JNK, anti-JNK, anti-phospho eIF2α, anti-eIF2α, anti-phospho c-Jun, anti-c-Jun, and horseradish peroxidase-conjugated secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Alexa Fluor^® 488 AffiniPure Goat Anti-Mouse IgG (Fcγ fragment specific) was supplied by Jackson ImmunoResearch Laboratories (West Grove, PA, USA). The Selleck Anti-Cancer Compound Library consisting of 414 drugs was purchased from the Department of Convergence Medicine, ASAN Medical Center, University of Ulsan College of Medicine (Seoul, Korea).

SH-BC-893 was synthesized by IntelliSyn RD (Montreal, Quebec, Canada) following the methods in (44 (link)) and with advice from S. Hanessian. The following chemicals were purchased: YM201636 (Cayman Chemicals, cat# 13576), apilimod (SelleckChem, cat# S6414), NAV2729 (R&D systems, cat# 5986), SecinH3 (Cayman Chemicals, cat# 10009570), perphenazine (PPZ, Sigma, cat# P6402-1G), UNC10217938A (Medchemexpress, cat# HY-136151), 6BIO (Cayman Chemicals, cat# 13123), AZD8055 (Cayman Chemicals, cat# 16978) and retro-2 (Sigma, cat# SML1085-5MG). Stock solutions were prepared as follows, aliquoted, and stored at –20°C: SH-BC-893 (5 mM in H₂O), YM201636 (1.6 mM in DMSO), apilimod (100 μM in DMSO), NAV2729 (12.5 mM in DMSO), SecinH3 (30 mM in DMSO), perphenazine (50 mM in DMSO), UNC10217938A (10 mM in DMSO), 6BIO (15 mM in DMSO), AZD8055 (1 mM in DMSO), and retro-2 (100 mM in DMSO). All chemical structures of compounds are shown in Supplementary Figure S1. Oligonucleotides used are shown in Supplementary Tables 1, 3 and 4 and were obtained from Ionis Pharmaceuticals.