Abi 394 synthesizer

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ABI 394 synthesizer is an automated instrument designed for the synthesis of oligonucleotides. It features programmable control of synthesis parameters and can produce a wide range of oligonucleotide lengths and sequences.

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Lab products found in correlation

Reversed phase hplc, by Agilent Technologies (1 mentions) C18 column, by Waters Corporation (1 mentions)

Close spelling variants of this product

ABI 394 DNA synthesizer (7 citations) ABI 394 (6 citations) ABI 394 DNA/RNA synthesizer (6 citations) 394 synthesizer (2 citations)

2 protocols using abi 394 synthesizer

Solid-Phase Synthesis of L-DNA and D-DNA

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Example 12

L-DNA nucleic acids or D-DNA nucleic acids were produced by solid-phase synthesis with an ABI 394 synthesizer (Applied Biosystems, Foster City, Calif., USA) using 2′TBDMS DNA phosphoramidite chemistry with standard exocyclic amine protecting groups (Damha and Ogilvie, 1993). For the DNA synthesis dA(N-Bz)-, dC(N—Ac)—, dG(N-ibu)-, and dT in the D- and L-configuration were applied. All phosphoramidites were purchased from ChemGenes, Wilmington, Mass. After synthesis and deprotection L-DNA nucleic acids or D-DNA nucleic acids were purified by gel electrophoresis.

US11015178B2. Enzymatic synthesis of L-nucleic acids (2021-05-25). Aptarion Biotech AG [DE]. Inventors: Andreas Pech [DE], Ralf David [DE], Florian Jarosch [DE], Michael Jahnz [DE], Sven Klussmann [DE].

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Oligonucleotide Synthesis and Purification

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Oligonucleotides were synthesized on an ABI 394 synthesizer (Applied Biosystems, Waltham, MA, USA), and then deprotected in AMA (ammonium hydroxide/40% aqueous methylamine, 1:1) at 65 °C for 30 min and further purified by reversed-phase HPLC (Agilent, Santa Clara, CA, USA) on a C-18 column (Waters, Milford, MA, USA) using 0.1 M triethylamine acetate (TEAA) buffer and acetonitrile as the eluents. ASO-P3 was prepared using universal CPG. The oligos were confirmed by the mass spectra shown in Supplementary Figures S2–S4.

Zhao H., Qin Y., Liu D., Geng X., Wang C., Ding D., Ding X., Xia Q., Liu J., Wang R, & Tan W. (2022). Site-Specific Radioiodination of Oligonucleotides with a Phenolic Element in a Programmable Approach. Molecules, 27(19), 6257.

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