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Thermo finnigan ltq orbitrap

Manufactured by Thermo Fisher Scientific
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The Thermo Finnigan LTQ-Orbitrap is a hybrid mass spectrometer that combines a linear ion trap (LTQ) and an Orbitrap mass analyzer. It provides high-resolution, accurate-mass (HR/AM) measurements for a wide range of applications, including proteomics, metabolomics, and small-molecule analysis.

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Lab products found in correlation

Xcalibur software, by Thermo Fisher Scientific (1 mentions) Pyridine d5, by Cambridge Isotopes (1 mentions)

5 protocols using thermo finnigan ltq orbitrap

1

Proteomic Analysis of Intestinal SRF KO

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Proteins prepared from jejunum of WT and Srf KO mice at KO15D (n = 6) were analyzed by two-dimensional LC-MS/MS on a Thermo Finnigan LTQ-Orbitrap (Thermo Scientific, Fremont, CA) and identified using Ingenuity Pathway Analysis (IPA, QIAGEN, Redwood City, CA) as previously described [5 (link)].
Lee M.Y., Park C., Ha S.E., Park P.J., Berent R.M., Jorgensen B.G., Corrigan R.D., Grainger N., Blair P.J., Slivano O.J., Miano J.M., Ward S.M., Smith T.K., Sanders K.M, & Ro S. (2017). Serum response factor regulates smooth muscle contractility via myotonic dystrophy protein kinases and L-type calcium channels. PLoS ONE, 12(2), e0171262.
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2

Quantitative Proteomics of SRF Knockout

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Soluble proteins were prepared from jejunum of WT and Srf KO mice. Proteins in the extracts were trypsin digested and analyzed by two-dimensional LC-MS/MS on a Thermo Finnigan LTQ-Orbitrap (Thermo Scientific, Waltham, MA, USA). Data were analyzed using Sequest and validated with Scaffold proteome software (Proteome Software, Portland, OR, USA). Proteins were quantified by spectral counting, and potentially perturbed molecular pathways were identified using Ingenuity Computational Pathway Analysis (Ingenuity, Redwood City, CA, USA). Differentially expressed proteins in Srf KO SMCs were obtained and analyzed with SRF-associated genes.
Lee M.Y., Park C., Berent R.M., Park P.J., Fuchs R., Syn H., Chin A., Townsend J., Benson C.C., Redelman D., Shen T.W., Park J.K., Miano J.M., Sanders K.M, & Ro S. (2015). Smooth Muscle Cell Genome Browser: Enabling the Identification of Novel Serum Response Factor Target Genes. PLoS ONE, 10(8), e0133751.
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3

Proteomics Analysis of SRF KO Mice

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Soluble proteins were prepared from jejunum tissues of WT and Srf KO mice (n=6). Each extract contained proteins from two mice. Proteins in the extracts were trypsin digested and analyzed by two-dimensional LC-MS/MS on a Thermo Finnigan LTQ-Orbitrap (Thermo Scientific, Fremont, CA, USA). Data were analyzed using Sequest (The Scripps Research Institute, La Jolla, CA, USA) and validated with Scaffold (Proteome Software, Portland, OR, USA). Proteins were quantified by spectral counting and potentially perturbed molecular pathways were found with Ingenuity Computational Pathway Analysis (IPA; QIAGEN, Redwood City, CA, USA).
Park C., Lee M.Y., Slivano O.J., Park P.J., Ha S., Berent R.M., Fuchs R., Collins N.C., Yu T.J., Syn H., Park J.K., Horiguchi K., Miano J.M., Sanders K.M, & Ro S. (2015). Loss of serum response factor induces microRNA-mediated apoptosis in intestinal smooth muscle cells. Cell Death & Disease, 6(12), e2011-.
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4

Coniferous Flavonoids Molecular Weights

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Molecular weights of the flavonoids in the two coniferous extracts were determined using an LC-MS/MS with a Thermo-Finnigan LTQ-Orbitrap instrument (Thermo Fisher Scientific, Waltham, MA, USA) at NICEM in Seoul National University (Seoul, Korea). Then, data acquisition was conducted with XcaliburTM software (ver. 4.3., Thermo Fisher Scientific Inc., MA, USA). In contrast, MS and MS/MS were operated through an electrospray ionization source in the negative ion mode, recorded in the range of 150 to 2000 m/z and 50 to 2000 m/z, respectively. Besides, nitrogen gas was used as the sheath gas, whose flow rate was kept at 10 L min−1. The capillary temperature was also maintained at 300 °C, nebulizer pressure was set at 45 psi, whereas fragmentation and capillary voltages were 0.2 kV and 4.5 kV, respectively. Additionally, the collision energy was set at 35 Ev, after which individual compounds were identified by comparing mass data and λmax results to previously reported values.
Gil C.S., Hong D., Duan S, & Eom S.H. (2022). Volatile and Non-Volatile Allelopathic Characteristics in Thermally Processed Needles of Two Conifers. Plants, 11(8), 1003.
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5

NMR and Mass Spectrometry Analysis

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NMR spectra were recorded on a Bruker Avance (400 and 500 MHz) spectrometer. A set of experiments was run including 1H, 13C, COSY, HSQC, HMBC, HSQC-TOCSY and NOESY. Pyridine-D5 (Cambridge Isotope Laboratories, Inc) was used as the NMR solvent and the obtained spectra were referenced using the internal signal from the solvent.
High resolution ESI-MS mass spectra for the isolated compound were obtained using Thermo-Finnigan LTQ Orbitrap (Thermo Scientific, Germany).
Raheem D.J., Tawfike A.F., Abdelmohsen U.R., Edrada-Ebel R, & Fitzsimmons-Thoss V. (2019). Application of metabolomics and molecular networking in investigating the chemical profile and antitrypanosomal activity of British bluebells (Hyacinthoides non-scripta). Scientific Reports, 9, 2547.
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