Anti lap2

Manufactured by BD

Anti-LAP2 is a lab equipment product developed by BD. It is a tool used to detect and measure the presence of LAP2 (Lamina-associated polypeptide 2) in biological samples.

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Lab products found in correlation

Anti nanog, by Abcam (3 mentions) Anti foxa2, by Cell Signaling Technology (3 mentions) Anti sma, by Merck Group (3 mentions) Anti ki67, by ZSGB-BIO (3 mentions) Anti tuj1, by Merck Group (3 mentions) Anti sox2, by Santa Cruz Biotechnology (3 mentions) Hoechst 33342, by Thermo Fisher Scientific (3 mentions) Anti lamin b, by Santa Cruz Biotechnology (2 mentions) Anti oct4, by Santa Cruz Biotechnology (2 mentions) Anti lamin a c, by Santa Cruz Biotechnology (2 mentions) Anti cd90 fitc, by BD (1 mentions) Anti cd105 apc, by BD (1 mentions) Anti p16, by BD (1 mentions) Anti cpd, by Cosmo Bio (1 mentions) Anti hcd31, by BD (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti nestin, by Merck Group (1 mentions) Anti cd73 pe, by BD (1 mentions) Anti igg apc, by BD (1 mentions)

3 protocols using anti lap2

Stem Cell Marker Expression Analysis

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The primary antibodies used were as follows (company, catalogue number): anti-ERCC6 (Abcam, ab96098), anti-NANOG (Abcam, ab21624), anti-SOX2 (Santa Cruz, sc-17320), anti-OCT4 (Santa Cruz, sc-5279), anti-SMA (Sigma, A5228), anti-TUJ1 (Sigma, T2200), anti-FOXA2 (Cell Signaling Technology, 8186S), anti-CD90-FITC (BD Bioscience, 555595), anti-CD73-PE (BD Bioscience, 550257), anti-CD105-APC (BD Bioscience, 17-1057-42), anti-IgG-FITC (BD Biosciences, 555748), anti-IgG-PE (BD Biosciences, 555749), anti-IgG-APC (BD Biosciences, 555751), anti-Lamin B (Santa Cruz, sc-6217), anti-LAP2 (BD Bioscience, 611000), anti-Ki67 (ZSGB-BIO, ZM0166), anti-P16 (BD Bioscience, 550834), anti-γ-H2AX (Millipore, 05-636), anti-Nestin (Millipore, MAB5326), anti-PAX6 (Covance, PRB-278P), anti-CPD (Cosmo Bio, TMD-2), anti-cleaved PARP (Cell Signaling Technology, 9541), anti-β-Actin (Santa Cruz, sc69879), anti-GAPDH (Santa Cruz, sc-25778), and anti-hCD31 (BD Bioscience, 555445).

Wang S., Min Z., Ji Q., Geng L., Su Y., Liu Z., Hu H., Wang L., Zhang W., Suzuiki K., Huang Y., Zhang P., Tang T.S., Qu J., Yu Y., Liu G.H, & Qiao J. (2019). Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction. Protein & Cell, 11(1), 1-22.

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Immunofluorescence Staining of Diverse Cellular Markers

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5 × 10⁵ cells were seeded on coverslips (Thermo Fisher Scientific), washed with PBS and fixed with 4% paraformaldehyde (PFA) for 30 min and permeabilized with 0.4% Triton X-100 in PBS for 30 min at RT. After washing with PBS three times, the cells were blocked with 10% donkey serum (Jackson ImmunoResearch) in PBS for 1 hr at RT. The cells were then incubated with primary antibodies in 10% donkey serum at 4°C overnight. Afterwards, cells were washed with PBS and stained with secondary antibodies and Hoechst 33342 (Thermo Scientific) for 1 hr at RT. A Leica SP5 confocal system was used for imaging. Antibodies used in this study were as follows: anti-Ki67 (ZSGB-BIO, ZM0166), anti-53BP1 (Bethyl Laboratories, A300-273A), anti-FOXA2 (Cell Signaling Technology, 8186S), anti-SMA (Sigma, A5228), anti-TuJ1 (Sigma, T2220), anti-H3K9me3 (Abcam, Ab8898), anti-NANOG (Abcam, Ab21624), anti-OCT3/4 (Santa Cruz, sc-5279), anti-SOX2 (Santa Cruz, sc-17320), and anti-LAP2 (BD Bioscience, 611000), anti-HP1α (Cell Signaling Technology, #2616S), and anti-Lamin A/C (Santa Cruz, sc-376248).

Hu H., Ji Q., Song M., Ren J., Liu Z., Wang Z., Liu X., Yan K., Hu J., Jing Y., Wang S., Zhang W., Liu G.H, & Qu J. (2020). ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin. Nucleic Acids Research, 48(11), 6001-6018.

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Immunofluorescence Staining of Stem Cell Markers

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Cells were fixed with 4% paraformaldehyde for 25 min, permeabilized with Triton X-100 (0.3% in PBS) for 25 min, incubated with blocking buffer (10% donkey serum in PBS) for 1 h at RT, and stained with primary antibodies overnight at 4 °C. Then, the cells were incubated with secondary antibodies for 1 h at RT. Hoechst 33342 (Invitrogen) was used to stain nuclear DNA. The primary antibodies used in immunofluorescence assays were as follows: anti-NANOG (Abcam, ab21624), anti-SOX2 (Santa Cruz, sc-17320), anti-OCT4 (Santa Cruz, sc-5279,), anti-SMA (Sigma, A5228), anti-TUJ1 (Sigma, T2200), anti-FOXA2 (Cell Signaling Technology, 8186S), anti-BIP (Cell Signaling Technology, 3177), anti-LaminA/C (Santa Cruz, sc-6215), anti-Calreticulin (Abcam, ab2907), anti-LaminB (Santa Cruz, sc-6217), anti-LAP2 (BD Bioscience, 611000), and anti-Ki67 (ZSGB-BIO, ZM0166).

Wang S., Hu B., Ding Z., Dang Y., Wu J., Li D., Liu X., Xiao B., Zhang W., Ren R., Lei J., Hu H., Chen C., Chan P., Li D., Qu J., Tang F, & Liu G.H. (2018). ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells. Cell Discovery, 4, 2.

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