Tall 1

Leukemia cell lines, BALL-1, HL-60, TALL-1, KG-1, THP-1, Kasumi, and K562 were purchased from Japanese Collection of Research Bioresources Cell Bank (National Institutes of Biomedical Innovation, Health and Nutrition, Japan). HMC-1, MV4-11, Ba/F3 and 293T cells were purchased from American Tissue Culture Collection (Manassas, VA). BALL-1, HL-60, TALL-1, KG-1, THP-1, Kasumi, K562, and HMC-1 cells were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal calf serum (FCS). Ba/F3 cells were cultured in RPMI 1640 medium supplemented with 10% FCS in the presence of 10 ng/ml IL-3. MV4-11 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM, Thermo Fisher Scientific) supplemented with 10% FCS. 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific) with 10% FCS.

PF-382, TALL-1, HPB-ALL, and Jurkat were purchased from DSMZ. CCRF-CEM and HEK293T cell lines were obtained from ATCC.
PF-382, TALL-1, CCRF-CEM, and Jurkat, were cultured in RPMI 1640 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), supplemented with 10% fetal bovine serum (Lonza Group Ltd., Basel, Switzerland), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific), and maintained at 37 °C in a 5% CO₂ atmosphere.
The HPB-ALL cell line was cultured in RPMI 1640 supplemented with 20% fetal bovine serum.
The HEK293T cell line was cultured in DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin.