Alpha smooth muscle actin α sma antibody

Atrial fibroblasts were fixed with 4% paraformaldehyde for 20 min at room temperature and then permeabilized with 0.5% Triton X-100 for 20 min at room temperature. The primary antibodies used in this experiment were incubated at 4 ℃ overnight, as follows: alpha-smooth muscle actin (α-SMA) antibody (1:100, Abcam, US) and then incubated with the following secondary antibodies (Beyotime, China, 1:200) for 90 min at room temperature. Nuclei were stained with DAPI (Beyotime, China). Cells were observed using a laser scanning confocal microscope (100x, ZEISS 510S, Germany).

Dexamethasone sodium phosphate and moxifloxacin hydrochloride were purchased from Dalian Meilun Biotechnology Co. Ltd., China, and genistein was obtained from Xian Xiaocao Plant Technology Co., Ltd., China. Compritol ATO 888 and Precirol ATO 5 were purchased from Gattefosse, France. Soybean oil was obtained from YiPusheng Pharmaceutical Co. Ltd., China and medium-chain triglyceride (MCT) was procured from CREMER OLEO GmbH & Co. KG. mPEG (2000)–PLA (2400) were synthesized by Changchun Institute of Applied Chemistry Chinese Academy of Science. Cremphor EL, Pluronic F68, Pluronic F127 and Kolliphor HS 15 were purchased from BASF corporation, Germany and anhydrous calcium chloride (CaCl₂) was provided by Tianjin Kermal Chemical Reagent Co. Ltd., China. Phosphate buffer saline (PBS) and Dulbecco’s minimum essential medium (DMEM) were purchased from BOSTER Biological Technology Co. Ltd., China. Basic fibroblast growth factor (bFGF) and transforming growth factor-β (TGF-β) were obtained from Gibco, China. Alpha-smooth muscle actin (α-SMA) antibody was provided by Abcam Corporation and proliferating cell nuclear antigen (PCNA) antibody, anti-Ki-67 antibody, GAPDH antibody were purchased from Proteintech Group. Inc. Acrylic Aspheric Intraocular Lens (IOL) were acquired from USIOL, Inc. All other reagents were of analytical grade.