Anti cav1

Manufactured by BD

Sourced in United States

Anti-Cav1 is a laboratory equipment product used for the detection and quantification of Cav1 protein. It is a research tool designed to assist in the study of cellular processes and signaling pathways involving Cav1.

Automatically generated - may contain errors

Lab products found in correlation

Anti actin, by Merck Group (1 mentions) Mouse monoclonal anti cav1, by BD (1 mentions) Rabbit polyclonal anti actin, by R&D Systems (1 mentions) Cell culture media and antibiotics, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti e cadherin, by BD (1 mentions) Plasmid midi kit, by Qiagen (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) Human fibronectin, by BD (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti von willebrand factor, by Santa Cruz Biotechnology (1 mentions) Anti bmprii, by Cell Signaling Technology (1 mentions) Anti p smad1 5 8, by Cell Signaling Technology (1 mentions) Antimouse and antirabbit hrp conjugated igg, by Cell Signaling Technology (1 mentions) Collagenase type 1, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Ripa buffer, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Heparin, by Merck Group (1 mentions) Anti β actin, by BD (1 mentions) Anti α sma, by Abcam (1 mentions)

Spelling variants of this product

Mouse monoclonal anti-Cav1 (2 citations) Anti-CAV1 #610060 (2 citations) Rabbit anti-CAV1 polyclonal antibody ( (2 citations) Anti-Cav1 rabbit Ab (2 citations)

7 protocols using anti cav1

Monoclonal Antibodies for Cavin-1 and Cav1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal antibodies recognizing cavin-1 (2F11) [28] (link) and Cav1 (7C8) [29] (link) have been previously described. The following antibodies were commercially acquired: anti-Cav1 was from BD Transduction Laboratories (San Jose, CA), anti-actin was from Sigma; anti-transferrin receptor was from Zymed Laboratories Inc. Invitrogen. Additional anti-cavin-1/PTRF antibodies were purchased from BD Transduction. Polyclonal rabbit anti-cavin-2/3 antibodies were produced against a peptide sequence at the C terminus of the proteins (21st Century Biochemicals, Hopkinton, MA).

Liu L., Hansen C.G., Honeyman B.J., Nichols B.J, & Pilch P.F. (2014). Cavin-3 Knockout Mice Show that Cavin-3 Is Not Essential for Caveolae Formation, for Maintenance of Body Composition, or for Glucose Tolerance. PLoS ONE, 9(7), e102935.

+ Open protocol

+ Expand

Antibody Characterization of Caveolin-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit polyclonal anti-CAV1, mouse monoclonal anti-CAV1, mouse monoclonal anti-pY14-CAV1, and mouse monoclonal anti-E-cadherin (BD Transduction Laboratories, Lexington, KY, USA), mouse monoclonal anti-PTPN14, and rabbit polyclonal anti-actin (R&D Systems, Minneapolis, MN, USA) antibodies were used as indicated by the manufacturers. Goat anti-rabbit and goat anti-mouse IgG antibodies coupled to horseradish peroxidase (HRP) were from Merck-Millipore (Billerica, Massachusetts, USA) and KPL Laboratories (Washington DC, USA), respectively. The ECL chemiluminescent substrate and the BCA protein determination kit were from Pierce (Rockford, IL, USA). The Plasmid Midi Kit was from Qiagen (Valencia, CA, USA). Human fibronectin was from Becton Dickinson (San Jose, CA, USA). Hygromycin was from Calbiochem (La Jolla, CA, USA). Fetal bovine serum (FBS) was from Biological Industries (Cromwell, CT, USA). Cell culture media and antibiotics were from GIBCO (Invitrogen, Carlsbad, CA, USA), Fugene 6® from Roche (Basel, Switzerland).

Díaz-Valdivia N.I., Díaz J., Contreras P., Campos A., Rojas-Celis V., Burgos-Ravanal R.A., Lobos-González L., Torres V.A., Perez V.I., Frei B., Leyton L, & Quest A.F. (2020). The non-receptor tyrosine phosphatase type 14 blocks caveolin-1-enhanced cancer cell metastasis. Oncogene, 39(18), 3693-3709.

+ Open protocol

+ Expand

Antibody and Reagent Sources for Cell Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit polyclonal anti‐α‐SMA was acquired from Abcam. Rabbit polyclonal anti‐GAPDH, anti‐von Willebrand Factor (vWF), and anti‐fibroblast‐specific protein 1 (FSP1) were acquired from Santa Cruz Biotechnology. Rabbit Polyclonal Endothelin‐1 was purchased from Fisher Scientific. Rabbit polyclonal anti‐BMPRII and anti‐P‐SMAD1/5/8 were purchased from Cell Signaling Technology. Mouse monoclonal anti‐eNOS, anti‐β‐actin and rabbit polyclonal anti‐Cav‐1 were purchased from BD PharMingen. Alexa‐Fluor 488 and 555‐conjugated goat and donkey antimouse or antirabbit IgG were purchased from Life Technologies. Antimouse and antirabbit HRP‐conjugated IgG were purchased from Cell Signaling Technology or Kierkegaard and Perry Laboratories (Supporting Information 1). RIPA buffer, protease, and phosphatase inhibitor cocktail, collagenase type I, paraformaldehyde, heparin, and sucrose were purchased from SIGMA Chemical, Co. Mounting media containing DAPI (VectaShield) was obtained from Vector. Stock solutions were prepared in 100% dimethylsulfoxide (DMSO), buffered physiological solution or sterile phosphate‐buffered saline (PBS) and diluted daily in sterile PBS or DMEM. The highest final concentration of the solvent was 0.1% (v/v) and did not affect the experiments. PCR primers were purchased from Integrated DNA Technologies, Inc.

Erewele E.O., Castellon M., Loya O., Marshboom G., Schwartz A., Yerlioglu K., Callahan C., Chen J., Minshall R.D, & Oliveira S.D. (2022). Hypoxia‐induced pulmonary hypertension upregulates eNOS and TGF‐β contributing to sex‐linked differences in BMPR2 +/R899X mutant mice. Pulmonary Circulation, 12(4), e12163.

+ Open protocol

+ Expand

Western Blotting and Immunofluorescence Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following commercially available antibodies were used for Western blotting: mouse monoclonal antibodies against clathrin heavy chain (CHC; BD Biosciences; 610500), lamin A/C (Santa Cruz Biotechnology; sc-7292), Hsp90 (Santa Cruz Biotechnology; sc-13119), EHD2 (Santa Cruz Biotechnology; sc-100724), dynamin (BD Biosciences; 610245), and filamin A (Chemicon; MAB1678); rabbit polyclonal antibodies against SUMO2/3 (Cell Signaling Technology; 4971), Cav1 (BD Biosciences; 610059), pacsin2 (Abgent; AP8088b); and cavin1 (Sigma; AV36965); for immunofluorescence, mouse monoclonal anti-cavin1 (BD Biosciences; 611258), goat polyclonal anti-EHD2 (Abcam; Ab23935), rabbit polyclonal anti-Cav1 (BD Biosciences; 610059), mouse monoclonal anti-lamin A/C (Santa Cruz Biotechnology; sc-7292), rabbit polyclonal anti-SUMO1 (Cell Signaling Technology; 4930), and rabbit polyclonal anti-SUMO2/3 (Cell Signaling Technology; 4971). Antibodies conjugated to Alexa Fluor 488, Cy3, Cy5, or HRP (Beckman Coulter and Invitrogen) were used as secondary antibodies. HaloTag dye JF635 was provided by L. Lavis (Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA). Accutase was purchased from Sigma-Aldrich. Hepes, SDS, and Tris were purchased from Euromedex.

Torrino S., Shen W.W., Blouin C.M., Mani S.K., Viaris de Lesegno C., Bost P., Grassart A., Köster D., Valades-Cruz C.A., Chambon V., Johannes L., Pierobon P., Soumelis V., Coirault C., Vassilopoulos S, & Lamaze C. (2018). EHD2 is a mechanotransducer connecting caveolae dynamics with gene transcription. The Journal of Cell Biology, 217(12), 4092-4105.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Vascular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sections were heated in citric acid buffer (pH 6.0) at 100°C for 10 min for antigen retrieval. Sections were then treated with 0.3% hydrogen for 30 min and blocked with 5% normal goat serum containing 0.05% Triton X‐100 (T‐PBS). Sections were then incubated overnight at 4°C with the following primary antibodies diluted in T-PBS: anti-Cav-1 (BD Biosciences, 610057, 610059), anti-Ki67 (Abcam, ab15580), or anti-cleaved caspase-3 (cell signaling, #9661). Isotype control that lacked specificity to the target but matched the class and type of the primary antibody was used as a negative control. After overnight incubation, the sections were incubated with Dako EnVision for 1 hour at room temperature and treated with Dako Liquid DAB+ Substrate Chromogen System (Dako; Carpinteria, CA) to detect the reaction products. Finally, the sections were counterstained with Dako Mayer’s Hematoxylin (Lillie’s Modification) Histological Staining Reagent (Dako). The integrated optical density of the immunoreactive signals in the vessel wall was analyzed using Image-J software. Relative density was graphed in arbitrary units. Cells staining positively for Ki67 and cleaved caspase-3 were directly counted in four high-power fields and the mean numbers of cells were then compared.

Hashimoto T., Isaji T., Hu H., Yamamoto K., Bai H., Santana J.M., Kuo A., Kuwahara G., Foster T.R., Hanisch J.J., Yatsula B.A., Sessa W.C., Hoshina K, & Dardik A. (2019). Stimulation of Caveolin-1 Signaling Improves Arteriovenous Fistula Patency. Arteriosclerosis, thrombosis, and vascular biology, 39(4), 754-764.

+ Open protocol

+ Expand

Immunofluorescence Staining of Vascular Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antigen retrieval was done in the same manner as IHC described above. After pretreatment, sections were blocked with 5% normal goat serum in T‐PBS for 1hr at room temperature and then incubated overnight at 4°C with the following primary antibodies diluted in T-PBS: anti-Cav-1 (BD Biosciences, 610057, 610059), anti-phospho-eNOS (Abcam, ab195944), anti-eNOS (Santa Cruz, sc654), anti-phospho-Akt1 (Cell Signaling Technology, 9018), anti-alpha-actin (Abcam, ab5694), anti-vWF (Abcam, ab11713), anti-PECAM-1 (Santa Cruz, sc1506) or with isotype-matched primary antibodies serving as negative controls. Secondary reagents were Alexa Fluor conjugated antibodies (488 and 568) from Life technologies used at 1:250. Sections were stained with SlowFade® Gold Antifade Mountant with DAPI (Life Technologies). Digital fluorescence images were captured and intensity of immunoreactive signal was measured using Image J software (NIH, Bethesda, Maryland). Intensity of merge signal was determined by applying a color threshold selective for yellow signal. For each antibody, standard quality control procedures were undertaken to optimize antigen retrieval, primary antibody dilution, secondary antibody detection, and other factors for both signal and noise.

+ Open protocol

+ Expand

Quantitative Analysis of Cav1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hippocampal CA3-CA1 regions were dissected from 1-day-old Sprague–Dawley rats, dissociated, plated onto poly-ornithine–coated 6-well dishes, and incubated for 14–18 days. At DIV8, neurons in three wells were transfected with control plasmids and neurons in the other three wells were transfected with shRNA-Cav1. Cells were further incubated until DIV14-18. All experiments were performed in parallel under the same conditions. Cells were lysed with lysis buffer containing 10 mM Tris (pH 7.4), 1% SDS, 10 mM NaF and 1 mM PMSF, supplemented with a protease inhibitor mixture (Complete Mini; Roche, Germany). The protein concentration in lysates was determined using a bicinchoninic acid (BCA) assay (Thermo, IL). Lysate samples containing equal amounts of protein were subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently transferred to polyvinylidene difluoride (PVDF) membranes. Membranes were blocked by incubating with 5% nonfat dry milk, and subsequently incubated with anti-Cav1 (BD Biosciences) and anti-β-actin (Santa Cruz, CA) primary antibodies. Band intensities were quantified densitometrically, and the [Cav1]/[β-actin] ratio was measured after background subtraction.

Koh S., Lee W., Park S.M, & Kim S.H. (2021). Caveolin-1 deficiency impairs synaptic transmission in hippocampal neurons. Molecular Brain, 14, 53.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!