Perkin elmer spectrum 400

Manufactured by PerkinElmer

Sourced in United States

The Perkin-Elmer Spectrum 400 is a Fourier Transform Infrared (FTIR) spectrometer. It is used to identify and analyze the chemical composition of substances by measuring their absorption or transmission of infrared radiation.

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Lab products found in correlation

Kaucl4, by Merck Group (1 mentions) Jms 700 mstation, by JEOL (1 mentions) Jem 1400, by JEOL (1 mentions) Glutaraldehyde, by Merck Group (1 mentions)

Close spelling variants of this product

Perkin-Elmer SpectrumTM 400 spectrometer Spectrum 400

4 protocols using perkin elmer spectrum 400

Synthesis and Characterization of Organometallic Complexes

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Fluorophenylthiols (HSR_F), Pb(CH₃COO)₂, K[AuCl₄], tetrahydrothiophene (tht), and 1,3-bis(diphenylphosphano) propane were purchased from Sigma-Aldrich and used without additional treatment. Solvents were obtained from JT Baker and used without previous treatment.
IR spectra were obtained using a Perkin-Elmer Spectrum 400 (PerkinElmer, Inc., Waltham, MA, USA) in the range of 4000 to 400 cm⁻¹ using attenuated total reflectance (ATR-FTIR). Elemental analysis was performed with a Thermo Scientific Flash 200 (Thermo Fisher Scientific., Waltham, MA, USA) at 950 °C. NMR spectra were recorded on a 9.4 T Varian VNMRS spectrometer (Varian, Inc., Palo Alto, CA, USA) in CDCl₃. Chemical shifts are reported in ppm relative to internal TMS δ = 0 ppm (¹H, ¹³C) and to external references of CFCl₃ (for ¹⁹F) and H₃PO₄ (for ³¹P) at 0 ppm. Positive-ion fast atom bombardment mass spectrometry (FAB+MS) spectra were measured on an MStation JMS-700 (JEOL, Ltd., Tokyo, Japan). Crystals were grown by slow (1 week) evaporation of solutions of the compounds in chloroform.

Moreno-Alcántar G., Salazar L., Romo-Islas G., Flores-Álamo M, & Torrens H. (2019). Exploring the Self-Assembled Tacticity in Aurophilic Polymeric Arrangements of Diphosphanegold(I) Fluorothiolates. Molecules, 24(23), 4422.

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Bacterial Cell Morphology Analysis

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The
morphologies of the bacterial cells were examined using a transmission
electron microscope (JEM-1400; JEOL, Tokyo, Japan). Samples were fixed
in 2% (v/v) glutaraldehyde (Merck, Darmstadt, Germany), dehydrated
to 30, 50, and 70% (v/v) twice in absolute ethanol, and then sectioned
using an ultramicrotome with a diamond knife.^{2 (link)} The EPS thickness was determined using Gatan digital micrograph
software. The average diameter was measured at different regions of
each cell for 16 bacteria. A Fourier transform infrared (FTIR) spectrum
of the lyophilized sample was obtained with the ATR technique using
a PerkinElmer Spectrum 400 (PerkinElmer, Inc., Shelton, CT). The spectrum
was scanned in the range of 4000–400 cm^–1 using four scans with a resolution of 4 cm^–1.
NMR spectra were recorded on a Varian Unity Inova 500 MHz spectrometer
(Varian NMR Systems, Palo Alto, CA), operating at 125 MHz for ¹³C NMR. The data were determined and processed using TopSpin
4.0 software. Thermogravimetric analysis (TGA) was performed using
Mettler Toledo equipment (Mettler Toledo, Schwarzenbach, Switzerland).
To determine the carbonization temperature, the samples were analyzed
in the range of 30–550 °C under a nitrogen atmosphere.

Choi I.S., Ko S.H., Lee M.E., Kim H.M., Yang J.E., Jeong S.G., Lee K.H., Chang J.Y., Kim J.C, & Park H.W. (2021). Production, Characterization, and Antioxidant Activities of an Exopolysaccharide Extracted from Spent Media Wastewater after Leuconostoc mesenteroides WiKim32 Fermentation. ACS Omega, 6(12), 8171-8178.

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Polyurethane FTIR Characterization

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The functional groups of the polyurethane were determined using Perkin-Elmer Spectrum 400 (Perkin Elmer, MA, USA) with 4 scans for each run in the range of 450 cm⁻¹ to 4000 cm⁻¹. The polyurethanes were placed onto the diamond crystal and subjected to FTIR scanning. The changes in the chemical structure of selected polyurethanes before and after enzymatic degradation were also evaluated.

Yeoh F.H., Lee C.S., Kang Y.B., Wong S.F., Cheng S.F, & Ng W.S. (2020). Production of Biodegradable Palm Oil-Based Polyurethane as Potential Biomaterial for Biomedical Applications. Polymers, 12(8), 1842.

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FTIR Analysis of Organic Derivatives

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FTIR of derivatives 5 and 7 were evaluated by FTIR in a PerkinElmer^® Spectrum 400 (PerkinElmer Inc, Waltham, MA, USA) equipped with an attenuated total reflectance (ATR) device. The ATR system was cleaned before each analysis by using dry paper and scrubbing it with methanol and water (50:50). The room air FTIR-ATR spectrum was used as background to verify the cleanliness and to evaluate the instrumental conditions and room interferences due to H₂O and CO₂. The spectra were obtained collecting 100 scans of each sample, between 4000 and 600 cm⁻¹, with a resolution of 4 cm⁻¹.

Ntungwe E., Domínguez-Martín E.M., Bangay G., Garcia C., Guerreiro I., Colombo E., Saraiva L., Díaz-Lanza A.M., Rosatella A., Alves M.M., Reis C.P., Passarella D, & Rijo P. (2021). Self-Assembly Nanoparticles of Natural Bioactive Abietane Diterpenes. International Journal of Molecular Sciences, 22(19), 10210.

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