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2 protocols using α glucosidae

1

Phytochemical Isolation and Evaluation

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Compounds 1, 2 and 3 (standards) were isolated from E. schimperi (Ahmed et al., 2017 ). Analytical grade reagents such as ABTS, DPPH, p-anisaldehyde, acarbose, α‐Amylase, α‐glucosidae and BHA were purchased from Sigma Aldrich. Other chemicals like dinitrosalicylic acid, sodium carbonate, p-nitrophenyl glucopyranoside and potassium persulphate are procured from different sources. Glass-backed silica gel 60F254 HPTLC plates (10 × 20 cm) were purchased from Merck (Darmstadt, Germany). CAMAG automatic TLC sampler-4 (CAMAG, Muttenz, Switzerland) was used to apply the standards and samples band wise. Development was done in ADC2 (automatic development chamber), scanning in CATS 4 (CAMAG) and documentation by CAMAG TLC Reprostar 3.
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2

Biochemical Analyses of Natural Compounds

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Phosphate‐buffered saline (PBS), 4‐Nitrophenyl β‐D‐glucopyranoside (pNPG), 3,5‐dinitro salicylic acid (DNS), caryophyllene, bovine serum albumin (BSA), α‐tocopherol, α‐amylase, α‐glucosidae, streptokinase, aspirin, 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH), and acarbose were purchased from Sigma‐Aldrich, Singapore. All other reagents and solvents were of analytical grade.
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