Gist hp c18 columns

Dopamine, N^ω-nitro-L-arginine methyl ester hydrochloride (L-NAME), and ascorbic acid were obtained from Sigma-Aldrich Chemicals Co. (USA). Adrenaline, L-741,626, norAdrenaline, ¹H-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), and U-46619 were purchased from Cayman Chemical Co. (USA). 6-NitroDopamine and 6-nitroDopamine-d₄ were acquired from Toronto Research Chemicals (Canada). Dopamine‐d₃ hydrochloride, DL‐norAdrenaline‐d₆ hydrochloride, and Adrenaline‐d₆ hydrochloride were acquired from CDN Isotopes (Canada). Strata™-X 33 mm Polymeric Reversed SPE cartridges were bought from Phenomenex (USA) and GIST-HP C₁₈ columns were obtained from Shimadzu (Germany). Sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl₂), magnesium sulfate (MgSO₄), sodium bicarbonate (NaHCO₃), potassium phosphate monobasic (KH₂PO₄), and glucose were acquired from Merck KGaA (Germany). Anti-S-100 (mouse monoclonal antibody, ab4066) and anti-calretinin (rabbit monoclonal antibody; ab92341) were obtained from Abcam.

6-Nitrodopamine-d₄ was bought from Toronto Research Chemicals Inc. (Toronto, Ontario, Canada). DPI, ebselen, GSK2795039, GKT137831, and resveratrol were purchased from Cayman Chemical Co. (Michigan, United States of America). Superoxide dismutase bovine, catalase from human erythrocytes, L-NAME, uric acid, and allopurinol were obtained from Sigma-Aldrich Chemicals Co. (St Louis, Missouri, United States of America). Dopamine-d₃ hydrochloride was acquired from CDN Isotopes (Canada). Hydrogen peroxide was bought from Exodo Cientifica (Sumaré, São Paulo, Brazil). Strata™-X 33 mm Polymeric Reversed SPE cartridges were bought from Phenomenex (United States of America), and GIST-HP C₁₈ columns were obtained from Shimadzu (Germany). Sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl₂), magnesium sulfate (MgSO₄), sodium bicarbonate (NaHCO₃), potassium phosphate monobasic (KH₂PO₄), and glucose were acquired from Merck KGaA (Darmstadt, Germany). The composition of the KHS was in mM: NaCl 118, KCl 4.7, CaCl₂ 2.5, MgSO₄ 1.2, NaHCO₃ 25, KH₂PO₄ 1.2 and dextrose 5.6.