The apoptosis of PTX-resistant SKOV3 and HeyA8 cells was determined using Annexin V-fluorescein isothiocyanate (Annexin V-FITC)/propidium iodide (PI) detection kit (Yeasen Biotech, Shanghai, China). In short, at 48 h after transfection, cells were collected via centrifuging. Cells were mixed with 100 μL binding buffer (Yeasen Biotech). After that, Annexin V-FITC (Yeasen Biotech) and PI (Yeasen Biotech) were employed to dye the cells. Finally, these samples were assessed using flow cytometry (BD Biosciences, San Diego, CA, USA).
Annexin 5 fluorescein isothiocyanate annexin 5 fitc propidium iodide detection kit
Manufactured by Yeasen
Sourced in China
The Annexin V-fluorescein isothiocyanate (Annexin V-FITC)/propidium iodide (PI) detection kit is a laboratory tool used to identify and quantify apoptotic and necrotic cells. Annexin V-FITC binds to phosphatidylserine, which is exposed on the cell surface during apoptosis, while PI stains the nuclei of necrotic cells. This kit provides a method to distinguish between viable, apoptotic, and necrotic cell populations through flow cytometry or fluorescence microscopy.
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2 protocols using annexin 5 fluorescein isothiocyanate annexin 5 fitc propidium iodide detection kit
1
Apoptosis Assay for PTX-Resistant Cells
2
Apoptosis Analysis by Annexin V-FITC/PI
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Annexin V-fluorescein isothiocyanate (Annexin V-FITC)/propidium iodide (PI) detection kit (Yeasen Biotech, Shanghai, China) was performed to determine cell apoptosis. In short, cells were digested and harvested at 48 h after treatment. Cells were washed with pre-cold PBS (Thermo Fisher) and centrifuged at 200 rpm for 5 min. Cells were resuspended in 100 μL binding buffer (Yeasen Biotech). Following that, cells were incubated with 5 μL Annexin V-FITC (Yeasen Biotech) and PI (Yeasen Biotech) for 12 min in dark. Results were assessed via flow cytometry (BD Biosciences, San Diego, CA, USA).
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