Paravision software version 6

To quantify tumor volume from MR images, the area of tumor on each slice of the images were delineated and regions of interest (ROIs) were drawn around the tumor volumes using Paravision software version 6.0 (Bruker-Biospin).

1 × 10⁶ LLC1-Luc-GFP cells were implanted into the 8-week-old FSP1;^creCAS9^EGFP mice. Cisplatin administration was started at a dose of 10.5 mg/kg i.p. twice per week when the tumors reached approximately 30 mm³ in volume. sgPrrx1 is administrated was started at a dose of MOI of 0.01 twice per week for 32 weeks duration, the mice were harvested, fixed in 10% normal buffered formalin and embedded in paraffin for subsequent analysis. Tumor size was measured every week using MRI imaging, and then the volume was calculated using using Paravision software version 6.0 (Bruker-Biospin). All mice used in this project were maintained under defined conditions at the Animal Experiment Center of Samsung Biomedical Research Institute, and all animal experiments were approved by the Animal Care and Use Committee of Samsung Biomedical Research Institute.