All laboratory grade chemicals were procured either from SD fine chemicals, HiMedia or Spectrochem, India and used as such without further purification. Sodium alginate was purchased from Himedia labs Mumbai. Waste pineapple shell was purchased from the local market of Nanded, Maharashtra.
Sodium alginate
Manufactured by HiMedia
Sourced in India
Sodium alginate is a water-soluble polysaccharide derived from brown seaweed. It functions as a thickening, gelling, and stabilizing agent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Calcium chloride, by HiMedia (4 mentions)
Sodium chloride (nacl), by HiMedia (3 mentions)
Chitosan, by HiMedia (2 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Guar gum, by HiMedia (1 mentions)
Glacial acetic acid, by HiMedia (1 mentions)
Orthophosphoric acid, by HiMedia (1 mentions)
Ferric chloride, by HiMedia (1 mentions)
Urea, by HiMedia (1 mentions)
Gelatin, by HiMedia (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Agno3, by Merck Group (1 mentions)
Ca no3 2, by Merck Group (1 mentions)
Sodium caseinate, by HiMedia (1 mentions)
Maltodextrin, by HiMedia (1 mentions)
Gum acacia, by HiMedia (1 mentions)
Sodium citrate, by HiMedia (1 mentions)
Ultra fast liquid chromatograph, by Shimadzu (1 mentions)
Di potassium hydrogen phosphate, by HiMedia (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
12 protocols using sodium alginate
1
Pineapple Shell Biopolymer Synthesis
2
Purification and Characterization of Peanut Allergenic Protein
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Sodium Dodecyl Sulphate and 8-anilino-1-napthalenesulfonic acid (ANS) were obtained from Sigma (USA). CTAB, sodium alginate and guar gum were obtained from Himedia.
Peanuts were purchased from a local store in Rourkela, and the PNA was purified using a previously described protocol [27] . The PNA purification was established using SDS-PAGE, where a single band of M wt 27.5 KDa was observed. The concentration of purified protein was calculated by taking specific absorbance at 280 nm (A 1%, 1cm ) of 7.7 [21] . All the other reagents used were of analytical grade. All the buffers and solutions were prepared in MilliQ water, and they were filtered through a 0.45 µm syringe filter before measurements. The molar concentration of ANS was determined spectrophotometrically by an extinction coefficient of 7800 M -1 cm -1 at 372 nm [28] . All the experiments were performed in 10 mM phosphate buffer (pH 7.4) at 25 °C.
Peanuts were purchased from a local store in Rourkela, and the PNA was purified using a previously described protocol [27] . The PNA purification was established using SDS-PAGE, where a single band of M wt 27.5 KDa was observed. The concentration of purified protein was calculated by taking specific absorbance at 280 nm (A 1%, 1cm ) of 7.7 [21] . All the other reagents used were of analytical grade. All the buffers and solutions were prepared in MilliQ water, and they were filtered through a 0.45 µm syringe filter before measurements. The molar concentration of ANS was determined spectrophotometrically by an extinction coefficient of 7800 M -1 cm -1 at 372 nm [28] . All the experiments were performed in 10 mM phosphate buffer (pH 7.4) at 25 °C.
3
Biopolymer-based Composite Hydrogel
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Chitosan (medium molecular weight ~1,90,000–3,10,000 daltons, <200 mPa.s in viscosity, deacetylation degree ≥75%), gelatin (50,000–1,00,000 daltons in molecular weight and 5.10–5.80 mPa.s in viscosity), sodium alginate (216.12 g/mol in molecular weight), urea, calcium chloride (CaCl2), acetic acid glacial, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, disodium hydrogen phosphate, sodium acetate, orthophosphoric acid, concentrated sulphuric acid, DiAcetylMonoxime (DAM), thiosemicarbazide, sodium chloride and ferric chloride was procured from Hi Media Laboratories Pvt. Ltd. Deionized water was used throughout the experiments.
4
Synthesis of Silver Alginate Beads
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Materials used in this research were silver nitrate salt (AgNO3; pro analytical grade, Merck KGaA, Darmstadt Germany), sodium alginate (molecular biology grade, Himedia, India), calcium nitrate tetrahydrate (Ca(NO3)2; analytical grade, Merck, Germany), 67% (w/w) nitric acid (HNO3; pro analytical grade, Merck, Germany), and hydrochloric acid (HCl; pro analytical grade, Merck, Germany). All chemicals were used without further purification.
5
Flaxseed Oil Encapsulation Protocol
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Flaxseed oil (Ceyon Healthcare India Private Limited, Lucknow, India) was used as active material. The edible grade wall materials used were: Maltodextrin, Sodium Caseinate, Gum Acacia and Sodium Alginate (Himedia, India).
6
Corn Cob Acid Hydrolysis Protocol
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Corn cob was provided by Abhyoday feedstock suppliers, Jalgaon, India. These are air-dried and milled into small particles (3.5 mm × 2.5 mm) before acid hydrolysis. Aminex column HPX87H (300 mm × 7.8 mm) was purchased from Biorad, Hercules, CA, USA. All used chemicals were of analytical grade. Sodium alginate, calcium chloride and sodium citrate were procured from Himedia, Mumbai.
7
Chiral Separation of Carvedilol Enantiomers
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Incubation was done in refrigerated shaker incubator of model Innova 4230, New Brunswick Scientific Co., Inc., (NJ, USA). Sample analysis was done by using a chiral column purchased from Phenomenex (USA) (Lux Cellulose-4; 250 × 4.6 mm; 5 µ particle size) and an Ultra Fast Liquid Chromatograph of Shimadzu (Kyoto, Japan) equipped with binary pump (LC 20AD), UV/Visible detector (LC 20A) and Rheodyne injector port. Lab solutions software was employed for the HPLC analysis.
Racemic Carvedilol was kindly gifted by Symed labs limited, Medak (India). (S)-(−)-Carvedilol and (R)-(+)-Carvedilol were purchased from the Toronto Pharmaceuticals (Canada). Calcium chloride, sodium alginate, sodium chloride and di-potassium hydrogen phosphate were purchased from Hi Media Laboratories Pvt. Ltd., Mumbai (India). Solvents (n-heptane, isopropanol and methanol) of HPLC grade were purchased from Sigma Aldrich Chemicals Pvt. Ltd., Maharashtra (India). Laboratory grade ethyl acetate was purchased from Finar limited, Ahmadabad, Gujarat (India).
Racemic Carvedilol was kindly gifted by Symed labs limited, Medak (India). (S)-(−)-Carvedilol and (R)-(+)-Carvedilol were purchased from the Toronto Pharmaceuticals (Canada). Calcium chloride, sodium alginate, sodium chloride and di-potassium hydrogen phosphate were purchased from Hi Media Laboratories Pvt. Ltd., Mumbai (India). Solvents (n-heptane, isopropanol and methanol) of HPLC grade were purchased from Sigma Aldrich Chemicals Pvt. Ltd., Maharashtra (India). Laboratory grade ethyl acetate was purchased from Finar limited, Ahmadabad, Gujarat (India).
8
Synthesis of SiO2 Nanoparticles
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Sodium alginate and gelatin purchased from HIMEDIA, Hyderabad, India. Calcium chloride was obtained from SD Fine Chem. Limited, India. 10× PBS (Sigma Aldrich, India) was diluted to 1× PBS and used in experiments. For the synthesis of SiO2 nanoparticles, reagents like tetraethyl orthosilicate (TEOS, 99%, Alfa Aesar), ethanol (99.98%, Pharmco-Aaper) and ammonia (30%, Sisco Research Laboratories) were used.
9
Biopolymer-Based Wound Healing Formulation
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Polyvinyl alcohol (PVA) and sodium alginate (SA) were purchased from Himedia Laboratories Pvt. Ltd. (Mumbai, India). Asiaticoside (AT) drug was purchased from Shaanxi Pioneer Biotech Co. Ltd. (Shaanxi, China). Dimethyl sulfoxide (DMSO) was obtained from S D Fine Chem. Ltd. (Mumbai, India). Streptozotocin (STZ) was bought from Sigma Aldrich (Bangalore, India). Formic acid was obtained from Avra Synthesis (Hyderabad, India). Ketamine was purchased from Miracalus Pharma Pvt. Ltd. (Mumbai, India). Silkworm cocoons were obtained as a gift sample from the Department of Zoology, Babasaheb Bhimrao Ambedkar University (Lucknow, India). All other used chemicals or reagents were of analytical grade.
10
Transdermal Finasteride and Testosterone Formulation
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Chitosan (low molecular weight), sodium alginate, calcium chloride, acetic acid, sodium hydroxide, carbopol 934, methyl paraben, propyl paraben, propylene glycol, triethanolamine, finasteride (all were procured from Hi-Media laboratories, Mumbai, India), testosterone (Loba Chemie Pvt Ltd., Mumbai, India), and carboxymethylcellulose (Sigma-Aldrich) were used. All chemicals and solvents used were of analytical reagent grade.
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