Foxm1

In this study, we used a panel of cell lines as listed in Supplementary Table 1. All of these cell lines were obtained from ATCC and were grown in the growth medium (listed in Supplementary Table 1). V5-tagged P53 mutant constructs (pLenti-6.0) pInducer20 were obtained from Addgene (listed in Supplementary Table 2). pCMV3 FOXM1 flag-tag was purchased from Sino Biologicals (Cat# HG12392-CF). Three siRNA oligonucleotides for each p53 (3′-GCAUGAACCGGAGGCCCAUTT-5′, 3′-CUACUUCCUGAAAACAACGTT-5′, and 3′-GACUCCAGUGGUAAUCUACTT-5′), E2F1A (Cat# SASI_Hs01_0039325904) and FOXM1 (Cat# SASI_Hs01_00243977, Cat# SASI_Hs01_00052108, and Cat# SASI_Hs01_00193782) were purchased from Sigma-Aldrich.

The cDNA molecules coding for various human MEIS2 isoforms were generated by reverse transcription using total RNA prepared from the human neuroblastoma cell line BE(2)-C, verified by sequencing, and cloned into the lentiviral expression vector pCDH-CMV-MCS-puro (SBI, Mountain View, CA, USA). The Retro-X Tet-Off Advanced Inducible Gene Expression System (Clontech, Mountain View, CA, USA) was used to generate BE(2)-C cells with inducible MEIS2d expression in the absence of doxycycline. Myc-tagged human MEIS2d was generated by PCR using pCDH-puro-MEIS2d, verified by sequencing, and subcloned into pRetroX-Tight-pur. pLKO.1-based lentiviral constructs expressing shRNA to MEIS2 (clone ID: TRCN0000016043, TRCN0000016044, TRCN0000016045, TRCN0000016046, and TRCN0000016047) were purchased from Open Biosystems (Huntsville, AL, USA) and to FOXM1 (clone ID: TRCN0000273984, TRCN0000015543, TRCN0000015544, TRCN0000015545, and TRCN0000015546) from Sigma-Aldrich (St. Louis, MO, USA). pLKO.1-shGFP (Addgene, Cambridge, MA, USA) was used as a control. Retroviruses and lentiviruses were produced in 293FT cells using the packaging plasmids pHDM-G and pMD.MLVogp (retroviruses) or pLP1, pLP2, and pLP/VSVG (lentiviruses).