Chicken anti-GFP (ab13970) antibody was purchased from Abcam. Rat anti-E-cadherin (131900) antibody was from Life Technologies. Alexa-Fluor-647-conjugated rabbit anti-cleaved caspase-3 (9602S) antibody was purchased from Cell Signaling Technology. Alexa-Fluor-568-conjugated phalloidin from Life Technologies was used at 1.0 U/mL. Alexa-488-conjugated secondary antibody was from Abcam, and Alexa-Fluor-568- and 647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. TMRM was obtained from Molecular Probes. Palmitic acid, stearic acid, linoleic acid, α-linolenic acid, sphingomyelin, cholesterol, and Nile Red were from Wako Pure Chemicals Industries. Acetylsalicylic acid (aspirin) was from Sigma-Aldrich. Trimetazidine (Abcam) was used at 10 μM.
Alexa fluor 568 and 647 conjugated secondary antibodies
Manufactured by Thermo Fisher Scientific
Alexa-Fluor-568- and 647-conjugated secondary antibodies are fluorescently labeled antibodies used for detection and visualization in various immunochemical techniques. These antibodies are designed to specifically bind to the Fc region of primary antibodies, allowing the detection of target proteins or cellular structures labeled with the primary antibodies. The Alexa-Fluor-568 and Alexa-Fluor-647 dyes provide bright and photostable fluorescent signals that can be detected using appropriate microscopy or flow cytometry equipment.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (5 mentions)
Alexa fluor 568 conjugated phalloidin, by Thermo Fisher Scientific (3 mentions)
Palmitic acid, by Fujifilm (2 mentions)
Stearic acid, by Fujifilm (2 mentions)
Linoleic acid, by Fujifilm (2 mentions)
Sphingomyelin, by Fujifilm (2 mentions)
Cholesterol, by Fujifilm (2 mentions)
Alexa 488 conjugated secondary antibody, by Abcam (2 mentions)
Chicken anti gfp ab13970 antibody, by Abcam (2 mentions)
Rat anti e cadherin 131900 antibody, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 647 conjugated rabbit anti cleaved caspase 3 9602s antibody, by Cell Signaling Technology (2 mentions)
α linolenic acid, by Fujifilm (2 mentions)
Nile red, by Fujifilm (2 mentions)
Acetylsalicylic acid aspirin, by Merck Group (2 mentions)
Trimetazidine, by Abcam (2 mentions)
Mouse anti filamin f6682 antibody, by Merck Group (2 mentions)
Mouse anti gapdh antibody 6c5 mab374, by Merck Group (1 mentions)
Rabbit anti zo 1 antibody 61 7300, by Thermo Fisher Scientific (1 mentions)
Peroxidase conjugated anti rabbit and anti mouse secondary antibodies, by GE Healthcare (1 mentions)
Trolox, by Bio-Techne (1 mentions)
5 protocols using alexa fluor 568 and 647 conjugated secondary antibodies
1
Immunolabeling of Apoptosis and Cytoskeleton
2
Immunofluorescence of Cytoskeletal Proteins
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Mouse anti-filamin (F6682) antibody was purchased from Sigma-Aldrich. Rabbit anti-p65 (sc-109) and mouse anti-EPLIN (sc-136399) antibodies were from Santa Cruz Biotechnology. Mouse anti-ADAMDEC1 antibody was kindly provided by Drs. Lund and Petersen (Novo Nordisk A/S)29 (link). For immunofluorescence, the primary antibodies were diluted in phosphate-buffered saline (PBS) containing 1% bovine serum albumin (BSA) at 1:100. Alexa-Fluor-568- and -647-conjugated secondary antibodies (Life Technologies) were used at 1:200. Alexa-Fluor-568- and -647-conjugated phalloidin (Life Technologies) was used at 1.0 U ml−1. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. Stable isotope-labeled amino acids were purchased from Cambridge Isotope Laboratories. The synthetic peptide containing stable isotope labeled phenylalanine (SLNSPEQEDF[13C9,15N]LQAEK) was obtained from SCRUM Inc.
3
Immunolabeling of Apoptosis and Cytoskeleton
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Chicken anti-GFP (ab13970) antibody was purchased from Abcam. Rat anti-E-cadherin (131900) antibody was from Life Technologies. Alexa-Fluor-647-conjugated rabbit anti-cleaved caspase-3 (9602S) antibody was purchased from Cell Signaling Technology. Alexa-Fluor-568-conjugated phalloidin from Life Technologies was used at 1.0 U/mL. Alexa-488-conjugated secondary antibody was from Abcam, and Alexa-Fluor-568- and 647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. TMRM was obtained from Molecular Probes. Palmitic acid, stearic acid, linoleic acid, α-linolenic acid, sphingomyelin, cholesterol, and Nile Red were from Wako Pure Chemicals Industries. Acetylsalicylic acid (aspirin) was from Sigma-Aldrich. Trimetazidine (Abcam) was used at 10 μM.
4
Antibody Characterization and Small Molecule Inhibitors
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Rabbit anti-FBP17 antibody was generated in a previous study (Itoh et al., 2005 (link)). Rabbit anti-GST antibody (sc-459) was purchased from Santa Cruz Biotechnology. Rabbit anti-DsRed antibody (632496) from Clontech and Rabbit anti-RFP antibody (PM005) from MBL were used for western blotting and immuno-EM, respectively. Mouse anti-GAPDH antibody (6C5, MAB374) was from Millipore. Rat anti-E-cadherin antibody (ECCD-2, 13-1900) and rabbit anti-ZO-1 antibody (61-7300) were from Thermo Fisher Scientific. Mouse anti-Filamin antibody (F6682) was from Sigma. Rabbit anti-phospho-Myosin light chain 2 (Thr18/Ser19) antibody (E2J8F, 95777) was from Cell Signaling Technology. Alexa Fluor-568- or -647-conjugated phalloidin (Invitrogen) was used at 1.0 U/ml. Alexa Fluor-568- and -647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. For immunofluorescence, primary antibodies were used at 1:100, except for GST antibody that was used at 1:50, and all secondary antibodies were used at 1:200. For western blotting, peroxidase-conjugated anti-rabbit and anti-mouse secondary antibodies were from GE Healthcare and Jackson ImmunoReseach, respectively. The selective Cdc42 inhibitor ML141 (20 μM) and the antioxidant Trolox (1 mM) were from Tocris Bioscience and Cayman Chemical, respectively.
5
Immunofluorescence Staining of Filamin
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Mouse anti-filamin (F6682) antibody was from Sigma-Aldrich (Gillingham, United Kingdom). Alexa Fluor 568–conjugated phalloidin (Life Technologies, Paisley, United Kingdom) was used at 1.0 U/ml. Alexa Fluor 568– and 647–conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5000. For immunofluorescence, primary antibody was used at 1:100, and all secondary antibodies were used at 1:200. Type I collagen (Cellmatrix Type I-A) was obtained from Nitta Gelatin (Osaka, Japan) and neutralized on ice to a final concentration of 2 mg/ml according to the manufacturer’s instructions. S1P, SPC, and bovine serum albumin (BSA) were from Sigma-Aldrich.
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