Cell activation cocktail pma ionomycin

Manufactured by BioLegend

Sourced in United States

The Cell Activation Cocktail PMA/Ionomycin is a laboratory reagent used to activate T cells and other immune cells in vitro. The product contains phorbol 12-myristate 13-acetate (PMA) and ionomycin, which stimulate protein kinase C and increase intracellular calcium levels, respectively, leading to the activation and proliferation of T cells and other lymphocytes.

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Lab products found in correlation

Lipopolysaccharides (lps), by Merck Group (1 mentions) Easysep human na ve cd4 t cell enrichment kit, by STEMCELL (1 mentions) Brefeldin a, by Thermo Fisher Scientific (1 mentions) Lymphocyte separation medium, by Lonza (1 mentions)

Close spelling variants of this product

PMA/ionomycin activation cocktail Ionomycin Cell Activation Cocktail PMA/Ionomycin cocktail Cell Activation Cocktail PMA/ionomycin Activation cocktail Ionomycin

2 protocols using cell activation cocktail pma ionomycin

Lung Cell Stimulation and Flow Cytometry

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Lung cell suspensions (3 × 10⁵ cells from Sham mice or 3 × 10⁴ cells from CLP-operated mice) were plated in 96 well plates and stimulated with either Cell Activation Cocktail PMA/Ionomycin at 1X (Biolegend, San Diego, USA) or 2 ng/ml of LPS (Sigma-Aldrich, St Louis, USA) in RPMI containing 10% FBS for 3 h at 37°C with 5% CO₂. Cells were recovered and washed with fresh PBS 1X and then stained for flow cytometry analysis.

Baudesson de Chanville C., Chousterman B.G., Hamon P., Laviron M., Guillou N., Loyher P.L., Meghraoui-Kheddar A., Barthelemy S., Deterre P., Boissonnas A, & Combadière C. (2020). Sepsis Triggers a Late Expansion of Functionally Impaired Tissue-Vascular Inflammatory Monocytes During Clinical Recovery. Frontiers in Immunology, 11, 675.

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Isolation and Activation of Naïve CD4+ T Cells

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Peripheral blood mononuclear cells (PBMCs) were isolated by gradient centrifugation with Lymphocyte Separation Medium (Lonza). CD4⁺CD45RA⁺ naive T cells were isolated from PBMCs with the EasySep Human Naïve CD4⁺ T Cell Enrichment Kit (STEMCELL Technologies). Purity of cell populations was consistently > 95%. Anti-CD3/anti-CD28-coated Dynabeads (Gibco) were used to activate naïve CD4⁺ T cells at a ratio of 2 cells: 1 bead for 72 h. Cell Activation Cocktail (PMA/Ionomycin) (Biolegend) was used to stimulate cytokine production in T cells with or without Brefeldin A (eBioscience) for 2 h.

Wu B., Zhao T.V., Jin K., Hu Z., Abdel M.P., Warrington K.J., Goronzy J.J, & Weyand C.M. (2021). Mitochondrial aspartate regulates TNF biogenesis and autoimmune tissue inflammation. Nature immunology, 22(12), 1551-1562.

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