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Du145 cells

Manufactured by Korean Cell Line Bank

DU145 cells are a human prostate cancer cell line derived from a brain metastasis. They are commonly used in cell biology research and drug testing. DU145 cells exhibit epithelial-like morphology and are androgen-independent.

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2 protocols using du145 cells

1

Cell Culture of Prostate and Fibrosarcoma

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Human prostate adenocarcinoma PC-3 cells (KCLB no. 21435), human prostate adenocarcinoma DU145 cells (KCLB no. 30081), and human fibrosarcoma HT1080 cells (KCLB no. 10121) were obtained from the Korean Cell Line Bank (Seoul, Korea). The cells were maintained in RPMI 1640 or DMEM (Cellgro, Manassas, VA, USA) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Cellgro) and penicillin (100 U/mL)/streptomycin (100 μg/mL) (Cellgro) at 37°C in a humidified 5% CO2 incubator.
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2

Culturing DU145 Prostate Cancer Cells

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DU145 cells were purchased from Korean Cell Line Bank (KCLB) (Seoul, Korea) and maintained in RPMI1640 medium (Welgene, Daegu, Korea), supplemented with 10% fetal bovine serum (Welgene, Daegu, Korea) and penicillin/streptomycin (WelGene, Daegu, Korea) at 37 °C with 5% CO2 in a humidified incubator.
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