In order to verify the potential effect of EOs on foodborne pathogens, E. coli ATCC 25922, S. aureus ATCC 14458, and S. enteritidis ATCC 13076 were used in this study. All microorganisms were obtained from the culture bank of the Oswaldo Cruz Foundation (FIOCRUZ, Rio de Janeiro, Brazil), and stored on nutrient agar (Kasvi, Italy) under refrigeration at the Center for Food Analysis (NAL) at the Federal University of Rio de Janeiro, where they were reactivated in 10 mL of brain heart infusion broth (BHI) (Kasvi, Spain) at 37 °C/18–24 h. After, strains were streaked on MacConkey agar (Kasvi, Spain), Baird Parker agar (Kasvi, Spain) supplemented with egg yolk tellurium (Sigma-Aldrich, Germany), and Xylose Lysine Deoxycholate agar (XLD) (Kasvi, Espanha) at 37 °C/18–24 h, respectively. Next, a characteristic colony of E. coli, S. aureus, and S. enteritidis were inoculated in individual tubes containing BHI broth and incubated at 37 °C/18–24 h for subsequent use in the assays described below.
Nutrient agar
Manufactured by Kasvi
Sourced in Italy, Brazil
Nutrient agar is a solid growth medium used for the cultivation and isolation of a wide range of microorganisms. It provides essential nutrients and growth factors required for the proliferation of bacteria, fungi, and other microbes. The agar base solidifies the medium, allowing for the formation of distinct colonies that can be easily observed and analyzed.
Automatically generated - may contain errors
Lab products found in correlation
Macconkey agar, by Kasvi (2 mentions)
Baird parker agar, by Kasvi (1 mentions)
Xylose lysine deoxycholate agar xld, by Kasvi (1 mentions)
Levofloxacin, by Merck Group (1 mentions)
Mueller hinton broth (mhb), by Kasvi (1 mentions)
α cyano 4 hydroxycinnamic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
2 protocols using nutrient agar
1
Evaluation of Essential Oils against Foodborne Pathogens
2
Antibacterial Compound Screening Protocol
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Colistin, gentamycin (Inlab, São Paulo, SP, Brazil) , ciprofloxacin, levofloxacin, αcyano-4-hydroxycinnamic acid, trifluoroacetic acid (TFA), acetonitrile (Sigma-Aldrich, Frankfurt, Germany), crystal violet, 95% ethanol, glucose, and sodium chloride (Synth, São Paulo, SP, Brazil) were purchased from commercial suppliers and used without further purification. Mueller-Hinton broth (MHB) and agar (MHA), nutrient agar, MacConkey agar, and trypticase soy broth (TSB) were purchased from Kasvi (São José do Pinhais, PR, Brazil). Apitoxin was provided by Ezequiel Dias Foundation (FUNED) and was collected following Benton's procedure (Benton et al. 1963) . Meal-worm beetle (Tenebrio molitor; Coleoptera) larvae were purchased from a company that is specialized in agricultural products (Mercado Central de Belo Horizonte, Minas Gerais, Brazil).
Polyvinyl chloride (PVC) urethral catheters (Embramed®; São Paulo, SP, Brazil) were obtained from stores specialized in hospital-medical supplies.
Polyvinyl chloride (PVC) urethral catheters (Embramed®; São Paulo, SP, Brazil) were obtained from stores specialized in hospital-medical supplies.
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