Mz apo

To analyze the geometric morphometrics, images of the head of 15 specimens were
captured by means of a stereo microscope Leica MZ APO with an increase of 20x
through the New Capture 2.0 program. Using the CLIC 98 program, eight anatomical
landmarks selected from Gurgel-Gonçalves et al.¹² were applied to each image. The analysis of the collected coordinates
was also made using the CLIC program 98 through an orthogonal method of
projections called Procrustes to adjust the figures to common points, i.e.,
translation to a common centroid, scaling to the same centroid size, and
rotation to minimize summed squared distances between the corresponding
landmarks.The discriminant analysis was performed using the major components of Procrustes.
A factorial map was built with the first two principal components (“eigenvalues”
CP1 and CP2) to observe the existence of differences between R.
neglectus and R. prolixus (Figure 1).
FIGURE 1:

This work is mainly based on specimens of Schizoprymnus collected by sweeping net and mostly Malaise traps (MT) set up in China. The key to the Chinese species of the genus Schizoprymnus is updated manually from Chou and Hsu, 1996. The terminology and measurements used follow van Achterberg (1988, 1993). Additional sources for the description of sculpture and setation are from Belokobylskij (1998). The following abbreviations are used: POL—postocellar line; OOL—ocular-ocellar line; OD—maximum diameter of lateral ocellus.
Descriptions and measurements were made under a stereomicroscope (Zeiss Stemi SV 6, ZEISS, Oberkochen, Germany). All figures were made by a camera (QImaging, Micropublisher, 3.3 RTV, QImaging, Surrey, BC, Canada) attached to a stereomicroscope (Leica MZ APO, Leica, Wetzlar, Germany) and Auto-Montage Pro version 5.0 software.
Type specimens and other materials are deposited in the Parasitic Hymenoptera Collection of the Zhejiang University, Hangzhou, China (ZJUH).

This study is based on specimens preserved in the Parasitic Hymenoptera Collection of Institute of Insect Sciences, Zhejiang University, Hangzhou, China (ZJUH), Institute of Zoology, Chinese Academy of Sciences, Beijing, China (IZCAS), Shanghai Entomological Museum, Chinese Academy of Sciences, Shanghai, China (SEMS), the Entomological Museum of the China Agricultural University, Beijing, China (CAU) and the Naturalis Biodiversity Center collection, Leiden, The Netherlands (RMNH).
The terminology and measurements used follow van Achterberg (1993) . All descriptions and measurements were made under a Zeiss Stemi 2000-C microscope; figures were made by a digital camera (Q-Imaging, Micropublisher, 3.3 RTV) attached to a stereomicroscope (Leica MZ APO, Germany) and Auto-Montage Pro version 5.0 software. Type specimens are deposited in the Parasitic Hymenoptera Collection of the Zhejiang University, Hangzhou, China (ZJUH).

The external morphology was observed with a dissecting stereoscope (SteREO Discovery V20, Zeiss and Leica Mz Apo). The following measurements were made with an ocular micrometer: total length, length from apical margin of clypeus to apex of elytra (TL); total width, width across both elytra at widest part (TW=EW); height, from the highest part of the beetle to elytral outer margins (TH); head width in front view, widest part (HW); pronotal length, from the middle of anterior margin to margin of basal foramen (PL); pronotal width at widest part (PW); elytral length, along suture, from the apex to the base including scutellum (EL). Male and female genitalia were dissected, cleared in 10% solution of NaOH by boiling for several minutes, and examined with an Olympus BX51 and Leica compound microscope.
Morphological characters were photographed with digital cameras (AxioCam HRc and Coolsnap–Procf & CRI Micro*Color), connected to the dissecting microscope. The software AxioVision Rel. 4.8 and Image-Pro Plus 5.1 were used to capture images from both cameras, and photos were cleaned up and laid out in plates with Adobe Photoshop CS 8.0.
Coccinellidae morphological terms follow Ślipiński (2007) and Ślipiński and Tomaszewska (2010) . Type specimens designated in the present paper are deposited at SCAU-the Department of Entomology, South China Agriculture University, Guangzhou, China.

Twenty-four hpf, wild type zebrafish embryos AB were soaked in embryo medium with 0.2 mM 1-phenyl 2-thiourea and incubated for further 24 h at 28.5 °C. At 48 hpf, embryos were dechorionated and anesthetized with 0.0003% tricaine prior to injection. Anesthetized embryos were positioned on a wet agarose 1% pad. The hindbrain of each embryo was injected with approximately 150–200 cells (ZsGreen-positive cells) using an Eppendorf FemtoJet microinjector, under the observation by stereoscope (MZ APO, Leica). After transplantation, embryos were incubated for 4 h at 32 °C and checked for the presence of fluorescent cells in the correct site. Then embryos were incubated at 32 °C in fresh medium for the following days. For the treatments, screened embryos were transferred in a 48-well plate with 1 mM compound concentration prior to the incubation at 32 °C. Five days post-fertilization (dpf, 3 days after injection), images of the tumors were captured and the relative integrated density, obtained by the product of the fluorescence intensity and the area of the tumor mass, was calculated as the ratio between the final and the initial tumor integrated density using ImageJ software.

Each sampled scale, preserved between two microscope slides stuck together by adhesive tape, was observed. Photographs were acquired through a stereomicroscope MZ APO (Leica, Wetzlar, Germany) equipped with a Moticam 10+ camera (Motic, Barcelona, Spain) using different magnifications according to the scale size. For M. surmuletus, the magnification was X6.3 (235 pixels/mm), for D. rerio, the magnification was X25 (932 pixels/mm), for all other species the magnification was X10 (371 pixels/mm). All scales were examined keeping the anterior portion leftwards and the dorsal portion upwards.